Human MMP-1 ELISA

RayBio® Human MMP-1 ELISA Kit for cell culture supernatants, heparin treated plasma, serum, and lysate samples. EDTA and Citrate are not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$420.00  
ELH-MMP1-1
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Assay Format

Detection Range:
8 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
Sensitivity:
8 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-2, - 3, -9, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P03956
Gene ID:
  • 4312
Gene Symbols:
  • CLG
  • MMP1
Protein Name & Synonyms:
Interstitial collagenase (EC 3.4.24.7) (Fibroblast collagenase) (Matrix metalloproteinase-1) (MMP-1) [Cleaved into: 22 kDa interstitial collagenase 27 kDa interstitial collagenase]
Target Species:
  • Human

Target Background

MMP-1 is a member of the large family of matrix metalloproteinase (MMP) that degrades substrates, such as gelatin, nidogen, collagen type 3, collagen type 4, laminin-1, proteoglycans, elastin, etc. Most MMP proteins are activated after being cleaved by extracellular proteinases. The matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological pathway: embryonic development, reproduction, tissue remodeling. MMP is also involved in disease processes, such as arthritis and metastasis.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Ariganello, Marianne B., et al. "Macrophage differentiation and polarization on a decellularized pericardial biomaterial." Biomaterials 32.2 (2011): 439-449.
    Species: Human
    Sample type: Cell Lysate (monocyte-derived macrophages)
  2. Ariganello, Marianne B., et al. "Macrophage differentiation and polarization on a decellularized pericardial biomaterial." Biomaterials 32.2 (2011): 439-449.
    Species: Human
    Sample type: Conditioned Media (monocyte-derived macrophages)
  3. van der Smissen, Anja, et al. "Growth promoting substrates for human dermal fibroblasts provided by artificial extracellular matrices composed of collagen I and sulfated glycosaminoglycans." Biomaterials 32.34 (2011): 8938-8946.
    Species: Human
    Sample type: Conditioned Media (Primary human dFb from healthy breast skin and foreskin)
  4. Angelini PD, Zacarias Fluck MF, Pederson K, et al. Constitutive HER2 signaling promotes breast cancer metastasis through cellular senescence. Cancer Res. 2013;73(1); 450-458.
    Species: Human
    Sample type: Conditioned Media (MCF7 (breast cancer) cell line, treated vs. untreated)
  5. Garratt L., Sutanto E., Ling K., et al. Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis. Eur Respir J. 2015 Aug;46(2):384-94. doi: 10.1183/09031936.00212114.
    Species: Human
    Sample type: BAL Fluid (CF and control patients)
  6. Zanotto-Filho A, Rajamanickam S, Loranc E, Masamsetti VP, Gorthi A, Romero JC, Tonapi S, Gonçalves RM, Reddick RL, Benavides R, Kuhn J, Chen Y, Bishop AJR. Sorafenib improves alkylating therapy by blocking induced inflammation, invasion and angiogenesis in breast cancer cells. Cancer Lett. 2018 Jul 1;425:101-115. doi: 10.1016/j.canlet.2018.03.037. Epub 2018 Mar 30. PMID: 29608984; PMCID: PMC5930000.
    Species: Human
    Sample type: Conditioned Media (Breast cancer cells)
  7. Chen, Da P., et al. "Activation of human fibroblast-like synoviocytes by uric acid crystals in rheumatoid arthritis." Cellular & molecular immunology 8.6 (2011): 469-478.
    Species: Human
    Sample type: Conditioned Media (FLS isolated from synovial tissues in RA patients)
  8. Yao H., et al. The PHSCN dendrimer as a more potent inhibitor of human breast cancer cell invasion, extravasation, and lung colony formation. Breast Cancer Res Treat. 2011 Jan;125(2):363-75. doi: 10.1007/s10549-010-0826-y.
    Species: Human
    Sample type:
  9. Decreased Mechanical Properties of Heart Valve Tissue Constructs Cultured in Platelet Lysate as Compared to Fetal Bovine Serum
    Species: Human
    Sample type:
  10. Tiraravesit N., Yakaew S., Rukchay R., et al. Artocarpus altilis heartwood extract protects skin against UVB in vitro and in vivo. J Ethnopharmacol. 2015 Dec 4;175:153-62. doi: 10.1016/j.jep.2015.09.023
    Species: Human
    Sample type: Conditioned Media (Skin cells isolated from cosmetic surgery patients.)
Very organized kit

BCM
Easy to use kit. Worked well for detection of MMP-1 in cell culture supernatant.

University Of Florida
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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