Human MMP-13 ELISA

RayBio® Human MMP-13 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$420.00  
ELH-MMP13-1
+

Assay Format

Detection Range:
6 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
6 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested Human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P45452
Gene ID:
  • 4322
Gene Symbols:
  • MMP13
Protein Name & Synonyms:
Collagenase 3 (EC 3.4.24.-) (Matrix metalloproteinase-13) (MMP-13)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Gotoh M., et al. Cyclic phosphatidic acid relieves osteoarthritis symptoms. Gotoh et al. Molecular Pain 2014, 10:52. doi:10.1186/1744-8069-10-52
    Species: Human
    Sample type: Conditioned Media (Synovial Tissue)
  2. Toprak, G., et al. "Fibrosis in heart failure subtypes." European Archives of Psychiatry and Clinical Neuroscience 266.4 (2015).
    Species: Human
    Sample type: Plasma
  3. Zhang X., Zhu Y., Chen X., Zhang Y., Zhang Y., et al. Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes. International Immunopharmacology, 2014 Epub Ahead of Print. http://dx.doi.org/10.1016/j.intimp.2014.05.006
    Species: Human
    Sample type: Conditioned Media (Human articular chondrocytes cell)
  4. Rhim E-M, Ahn S-J, Kim J-Y, et al. Stimulation of Matrix Metalloproteinases by Tumor Necrosis Factor-? in Human Pulp Cell Cultures. J Endodontics. 2013 Jun;39(6):795-800.
    Species: Human
    Sample type: Conditioned Media (dental pulp cells)
  5. Toegel,S. et al. Caesalpinia sappan extract inhibits IL1?-mediated overexpression of matrix metalloproteinases in human chondrocytes.Genes Nutr. 2012 Apr;7(2):307-18. doi: 10.1007/s12263-011-0244-8. Epub 2011 Aug 18.
    Species: Human
    Sample type: Conditioned Media (Primary chondrocytes and SW1353 cells)
  6. Yuan W, Zhao M-D, Yuan F-L, et al. Association of endothelin-1 expression and cartilaginous endplate degeneration in humans. PLoS ONE. 2013;8(4):e60062.
    Species: Human
    Sample type: Conditioned Media (cartilaginous endplate cells)
  7. Soydinc, Hatice Ender, et al. "Prolidase, matrix metalloproteinases 1 and 13 activity, oxidative-antioxidative status as a marker of preterm premature rupture of membranes and chorioamnionitis in maternal vaginal washing fluids." International journal of medical sciences 10.10 (2013): 1344.
    Species: Human
    Sample type: Vaginal Washing Fluid
  8. Chijimatsu R., Kunugiza Y., Taniyama Y., et al. Expression and pathological effects of periostin in human osteoarthritis cartilage. BMC Musculoskelet Disord. 2015; 16: 215. Published online 2015 Aug 21. doi: 10.1186/s12891-015-0682-3
    Species: Human
    Sample type: Conditioned Media (Human Chondrocytes)
  9. Balasubramanian SA, Pye DC, Willcox MDP. Effects of eye rubbing on the levels of protease, protease activity and cytokines in tears: relevance in keratoconus. Clin Exp Optom. 2013;96(2):214-218.
    Species: Human
    Sample type: Tears (before and after eye-rubbing)
  10. Jacob J., Eisemon E., Sheibani-Rad S., et al. Matrix metalloproteinase levels as a marker for rotator cuff tears. Orthopedics. 2012 Apr;35(4):e474-8. doi: 10.3928/01477447-20120327-18.
    Species: Human
    Sample type: Tissue Lysate (Human rotator cuff tisse lysate)

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

Customers Also Bought Customers Also Bought