Human MMP-2 ELISA

RayBio® Human MMP-2 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$756.00  
ELH-MMP2-2
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Assay Format

Detection Range:
3.5 ng/ml - 800 ng/ml
Recommended Dilution (serum & plasma):
5 - 50 fold
Sensitivity:
3,500 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 3, -7, -8, -10, -12, -13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P08253
Gene ID:
  • 4313
Gene Symbols:
  • CLG4A
  • MMP2
Protein Name & Synonyms:
72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) (TBE-1) [Cleaved into: PEX]
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Chim H., Armijo BS., Miller E., et al. Propranolol induces regression of hemangioma cells through HIF-1?-mediated inhibition of VEGF-A. Ann Surg. 2012 Jul;256(1):146-56. doi: 10.1097/SLA.0b013e318254ce7a.
    Species: Human
    Sample type: Conditioned Media (Hemangioma endothelial cells treated with propranolol)
  2. Moir, L. M., et al. "Doxycycline inhibits matrix metalloproteinase?2 secretion from TSC2?null mouse embryonic fibroblasts and lymphangioleiomyomatosis cells." British journal of pharmacology 164.1 (2011): 83-92.
    Species: Human
    Sample type: Conditioned Media
  3. Garratt L., Sutanto E., Ling K., et al. Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis. Eur Respir J. 2015 Aug;46(2):384-94. doi: 10.1183/09031936.00212114.
    Species: Human
    Sample type: BAL Fluid (CF and control patients)
  4. Al?Rakan, Maha A., et al. "Breast stromal fibroblasts from histologically normal surgical margins are pro?carcinogenic." The Journal of pathology 231.4 (2013): 457-465.
    Species: Human
    Sample type: Cell Lysate (SFCMs from CAF, TCF and NBF cells)
  5. Wang X., Zhou Q., Lu Y., et al. Glycyrrhetinic acid potently suppresses breast cancer invasion and metastasis by impairing the p38 MAPK-AP1 signaling axis. Expert Opin Ther Targets. 2015 May;19(5):577-87. doi: 10.1517/14728222.2015.1012156.
    Species: Human
    Sample type: Conditioned Media (Breast cancer cell lines treated with Glycyrrhetinic acid)
  6. Tammali, Ravinder, et al. "Inhibition of aldose reductase prevents angiogenesis in vitro and in vivo." Angiogenesis 14.2 (2011): 209-221.
    Species: Human
    Sample type: Conditioned Media
  7. Hampel U, Klonisch T, Sel S, et al. Insulin-like Factor 3 Promotes Wound Healing at the Ocular Surface. Endocrinology. 2013;154(6):2034-2045.
    Species: Human
    Sample type: Conditioned Media (sebaceous cell line, conjunctival epithelial cell line, corneal epithelial cell line)
  8. Slivka P., et al. Fractionation of an ECM hydrogel into structural and soluble components reveals distinctive roles in regulating macrophage behavior. Biomater. Sci., 2014, Advance Article DOI: 10.1039/C4BM00189C
    Species: Human
    Sample type: Tissue Lysate (Extracellular matrix lysate)
  9. Domienik-Kar?owicz J., et al. Emerging Markers of Atherosclerosis Before and After Bariatric Surgery. Obesity Surgery Sept 2014. epub ahead of print. DOI:10.1007/s11695-014-1407-7
    Species: Human
    Sample type: Serum
  10. Toprak, G., et al. "Fibrosis in heart failure subtypes." European Archives of Psychiatry and Clinical Neuroscience 266.4 (2015).
    Species: Human
    Sample type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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