Human MMP-3 ELISA

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RayBio® Human MMP-3 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

ELH-MMP3-1
+

Antigen Information

Accession Number:
  • P08254
Gene ID:
  • 4314
Gene Symbols:
  • MMP3
  • STMY1
Protein Name & Synonyms:
Stromelysin-1 (SL-1) (EC 3.4.24.17) (Matrix metalloproteinase-3) (MMP-3) (Transin-1)
Target Species:
  • Human

Assay Format

Detection Range:
0.3 ng/ml - 100 ng/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
Sensitivity:
300 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -7, -8, -9, -10, -12, -13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Abd-Allah S., El-Shal A., Shalaby S., et al. Influence of Matrix metalloproteinase 1 and 3 genetic variations on susceptibility and severity of juvenile idiopathic arthritis. IUBMB Life. 2015 Dec;67(12):934-42. doi: 10.1002/iub.1446.
    Species: Human
    Sample type: Serum
  2. Galil S., et al. Baseline serum level of matrix metalloproteinase-3 as a biomarker of progressive joint damage in rheumatoid arthritis patients. International Journal of Rheumatic Disease, October 2014. Epub ahead of print. DOI: 10.1111/1756-185X.12434
    Species: Human
    Sample type: Serum
  3. Gotoh M., et al. Cyclic phosphatidic acid relieves osteoarthritis symptoms. Gotoh et al. Molecular Pain 2014, 10:52. doi:10.1186/1744-8069-10-52
    Species: Human
    Sample type: Conditioned Media
  4. Zhang X., Zhu Y., Chen X., Zhang Y., Zhang Y., et al. Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes. International Immunopharmacology, 2014 Epub Ahead of Print. http://dx.doi.org/10.1016/j.intimp.2014.05.006
    Species: Human
    Sample type: Conditioned Media
  5. Rhim E-M, Ahn S-J, Kim J-Y, et al. Stimulation of Matrix Metalloproteinases by Tumor Necrosis Factor-? in Human Pulp Cell Cultures. J Endodontics. 2013 Jun;39(6):795-800.
    Species: Human
    Sample type: Conditioned Media
  6. Raychaudhuri S., Raychauduri S., Genovese M. IL-17 receptor and its functional significance in psoriatic arthritis. Mol Cell Biochem. 2012 Jan;359(1-2):419-29. doi: 10.1007/s11010-011-1036-6.
    Species: Human
    Sample type: Conditioned Media
  7. Jacob J., Eisemon E., Sheibani-Rad S., et al. Matrix metalloproteinase levels as a marker for rotator cuff tears. Orthopedics. 2012 Apr;35(4):e474-8. doi: 10.3928/01477447-20120327-18.
    Species: Human
    Sample type: Tissue Lysate
  8. Abd-Allah S., Shalaby S., Pasha H., et al. Variation of matrix metalloproteinase 1 and 3 haplotypes and their serum levels in patients with rheumatoid arthritis and osteoarthritis. Genet Test Mol Biomarkers. 2012 Jan;16(1):15-20. doi: 10.1089/gtmb.2011.0003
    Species: Human
    Sample type: Serum
  9. Bassiouni HM., et al. Phonoarthrography, musculoskeletal ultrasonography, and biochemical biomarkers for the evaluation of knee cartilage in osteoarthritis. Mod Rheumatol. 2011 Oct;21(5):500-8. doi: 10.1007/s10165-011-0441-8.
    Species: Human
    Sample type:
  10. Yoo J., et al. Protein kinase D1 mediates synergistic MMP-3 expression induced by TNF-? and bradykinin in human colonic myofibroblasts. Biochem Biophys Res Commun. 2011 Sep 16;413(1):30-5. doi: 10.1016/j.bbrc.2011.08.029.
    Species: Human
    Sample type:
Good product with reliable results and great tech support.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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