Human MMP-3 ELISA

RayBio® Human MMP-3 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$756.00  
ELH-MMP3-2
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Assay Format

Detection Range:
0.3 ng/ml - 100 ng/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
Sensitivity:
300 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -7, -8, -9, -10, -12, -13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P08254
Gene ID:
  • 4314
Gene Symbols:
  • MMP3
  • STMY1
Protein Name & Synonyms:
Stromelysin-1 (SL-1) (EC 3.4.24.17) (Matrix metalloproteinase-3) (MMP-3) (Transin-1)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Zanotto-Filho A, Rajamanickam S, Loranc E, Masamsetti VP, Gorthi A, Romero JC, Tonapi S, Gonçalves RM, Reddick RL, Benavides R, Kuhn J, Chen Y, Bishop AJR. Sorafenib improves alkylating therapy by blocking induced inflammation, invasion and angiogenesis in breast cancer cells. Cancer Lett. 2018 Jul 1;425:101-115. doi: 10.1016/j.canlet.2018.03.037. Epub 2018 Mar 30. PMID: 29608984; PMCID: PMC5930000.
    Species: Human
    Sample type: Conditioned Media (Breast cancer cells)
  2. Gotoh M., et al. Cyclic phosphatidic acid relieves osteoarthritis symptoms. Gotoh et al. Molecular Pain 2014, 10:52. doi:10.1186/1744-8069-10-52
    Species: Human
    Sample type: Conditioned Media (Synovial Tissue)
  3. Zhang X., Zhu Y., Chen X., Zhang Y., Zhang Y., et al. Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes. International Immunopharmacology, 2014 Epub Ahead of Print. http://dx.doi.org/10.1016/j.intimp.2014.05.006
    Species: Human
    Sample type: Conditioned Media (Human articular chondrocytes cell)
  4. Rhim E-M, Ahn S-J, Kim J-Y, et al. Stimulation of Matrix Metalloproteinases by Tumor Necrosis Factor-? in Human Pulp Cell Cultures. J Endodontics. 2013 Jun;39(6):795-800.
    Species: Human
    Sample type: Conditioned Media (dental pulp cells)
  5. Yoo J., et al. Protein kinase D1 mediates synergistic MMP-3 expression induced by TNF-? and bradykinin in human colonic myofibroblasts. Biochem Biophys Res Commun. 2011 Sep 16;413(1):30-5. doi: 10.1016/j.bbrc.2011.08.029.
    Species: Human
    Sample type:
  6. Upadhyay A, Sharma G, Kivivuori S, Raye W S, Zabihi E, et al. Role of a LIF antagonist in LIF and OSM induced MMP-1, MMP-3, and TIMP-1 expression by primary articular chondrocytes. Cytokine. 2009;46:332-338.
    Species: Porcine
    Sample type: Conditioned Media (Primary chondrocytes cell supernate)
  7. Upadhyay A., et al. Role of a LIF antagonist in LIF and OSM induced MMP-1, MMP-3, and TIMP-1 expression by primary articular chondrocytes. Cytokine. 2009 Jun;46(3):332-8. doi: 10.1016/j.cyto.2009.03.001.
    Species: Human
    Sample type:
  8. García-Arnandis, Isabel, et al. "Control of cell migration and inflammatory mediators production by CORM-2 in osteoarthritic synoviocytes." PLoS One 6.9 (2011): e24591.
    Species: Human
    Sample type: Conditioned Media (Synoviocytes)
  9. Sommerfelt, Randi M., et al. "Cytosolic phospholipase A2 regulates TNF-induced production of joint destructive effectors in synoviocytes." PloS one 8.12 (2013): e83555.
    Species: Human
    Sample type: Conditioned Media (synovial sarcoma derived cell line SW982 )
  10. Abd-Allah S., El-Shal A., Shalaby S., et al. Influence of Matrix metalloproteinase 1 and 3 genetic variations on susceptibility and severity of juvenile idiopathic arthritis. IUBMB Life. 2015 Dec;67(12):934-42. doi: 10.1002/iub.1446.
    Species: Human
    Sample type: Serum (Susceptibility to juvenile idiotathic arthritis)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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