RayBio® Human MMP-9 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.


Antigen Information

Accession Number:
  • P14780
Gene ID:
  • 4318
Gene Symbols:
  • CLG4B
  • MMP9
Protein Name & Synonyms:
Matrix metalloproteinase-9 (MMP-9) (EC (92 kDa gelatinase) (92 kDa type IV collagenase) (Gelatinase B) (GELB) [Cleaved into: 67 kDa matrix metalloproteinase-9 82 kDa matrix metalloproteinase-9]
Target Species:
  • Human

Assay Format

Detection Range:
10 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
300 - 3,000 fold
10 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Kilickesmez KO., et al. Dilatation of the ascending aorta and serum alpha 1-antitrypsin level in patients with bicuspid aortic valve. Heart Vessels (2012) 27:391-397 DOI 10.1007/s00380-011-0161-1
    Species: Human
    Sample type: Serum
  2. Sundrani DP, Chavan-Gautam PM, Pisal HR, Mehendale SS, Joshi SR (2012) Matrix Metalloproteinase-1 and -9 in Human Placenta during SpontaneousVaginal Delivery and Caesarean Sectioning in Preterm Pregnancy. PLoS ONE 7(1): e29855. doi:10.1371/journal.pone.0029855
    Species: Human
    Sample type: Tissue Lysate
  3. Zhu P., Ren M., Yang C., et al. Involvement of RAGE, MAPK and NF-?B pathways in AGEs-induced MMP-9 activation in HaCaT keratinocytes. Exp Dermatol. 2012 Feb;21(2):123-9. doi: 10.1111/j.1600-0625.2011.01408.x.
    Species: Human
    Sample type: Conditioned Media
  4. Cherneva, RV. Et al. The role of small heat-shock protein ?B-crystalline (HspB5) in COPD pathogenesis. Int J Chron Obstruct Pulmon Dis. 2012;7:633-40. doi: 10.2147/COPD.S34929. Epub 2012 Oct 4.
    Species: Human
    Sample type: Plasma
  5. Fuchs S., Lavi I., Tzang O., et al. Intracoronary monocyte chemoattractant protein 1 and vascular endothelial growth factor levels are associated with necrotic core, calcium and fibrous tissue atherosclerotic plaque components: an intracoronary ultrasound radiofrequency study. Cardiology. 2012;123(2):125-32. doi: 10.1159/000342050.
    Species: Human
    Sample type: Serum
  6. Wyrebska A., Gach K., Szemraj J., et al. Comparison of anti-invasive activity of parthenolide and 3-isopropyl-2-methyl-4-methyleneisoxazolidin-5-one (MZ-6)--a new compound with ?-methylene-?-lactone motif--on two breast cancer cell lines. Chem Biol Drug Des. 2012 Jan;79(1):112-20. doi: 10.1111/j.1747-0285.2011.01257.x.
    Species: Human
    Sample type: Conditioned Media
  7. Kaminska-Winciorek G., Deja G., Polanska J., Jarosz-Chobot P. The role of selected metalloproteinases in cheiroarthropathy in children with type 1 diabetes - a pilotage study. Int J Clin Pract. 2012 Apr;66(4):374-7. doi: 10.1111/j.1742-1241.2011.02702.x.
    Species: Human
    Sample type: Serum
  8. Leng L. Jiang T., Zhang Y. TLR9 expression is associated with prognosis in patients with glioblastoma multiforme. J Clin Neurosci. 2012 Jan;19(1):75-80. doi: 10.1016/j.jocn.2011.03.037.
    Species: Human
    Sample type: Conditioned Media
  9. Dabrowski W., Rzecki Z., Czajkowski M., et al. Volatile anesthetics reduce biochemical markers of brain injury and brain magnesium disorders in patients undergoing coronary artery bypass graft surgery. J Cardiothorac Vasc Anesth. 2012 Jun;26(3):395-402. doi: 10.1053/j.jvca.2011.10.014
    Species: Human
    Sample type: Serum
  10. Jacob J., Eisemon E., Sheibani-Rad S., et al. Matrix metalloproteinase levels as a marker for rotator cuff tears. Orthopedics. 2012 Apr;35(4):e474-8. doi: 10.3928/01477447-20120327-18.
    Species: Human
    Sample type: Tissue Lysate

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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