Human MMP Array C1

RayBio® C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.


Assay Format

  • Akt Signaling
  • HIF-1 alpha Signaling
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

4 or 8 Sample Kit

Protein Information

Gene Symbols:
  • MMP1
  • MMP10
  • MMP13
  • MMP2
  • MMP3
  • MMP8
  • MMP9
  • TIMP1
  • TIMP2
  • TIMP4

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Cheng YC, Chiang HY, Cheng SJ, Chang HW, Li YJ, Shieh SY. Loss of the tumor suppressor BTG3 drives a pro-angiogenic tumor microenvironment through HIF-1 activation. Cell Death Dis. 2020 Dec 11;11(12):1046. doi: 10.1038/s41419-020-03248-5. PMID: 33311481; PMCID: PMC7732837.
    Species: Mouse
    Sample type: Conditioned media
  2. Tinaburri L, Valente C, Teson M, Minafò YA, Cordisco S, Guerra L, Dellambra E. The Secretome of Aged Fibroblasts Promotes EMT-Like Phenotype in Primary Keratinocytes from Elderly Donors through BDNF-TrkB Axis. J Invest Dermatol. 2020 Sep 12:S0022-202X(20)32062-5. doi: 10.1016/j.jid.2020.08.019. Epub ahead of print. PMID: 32931807.
    Species: Human
    Sample type: Conditioned Media
  3. Kc P, Shah M, Shaik R, Hong Y, Zhang G. Preseeding of Mesenchymal Stem Cells Increases Integration of an iPSC-Derived CM Sheet into a Cardiac Matrix. ACS Biomater Sci Eng. 2020 Dec 14;6(12):6808-6818. doi: 10.1021/acsbiomaterials.0c00788. Epub 2020 Nov 4. PMID: 33320624.
    Species: Human
    Sample type: Conditioned Media
  4. Chien YC., Liu LC., Ye HY., et al. EZH2 promotes migration and invasion of triple-negative breast cancer cells via regulating TIMP2-MMP-2/-9 pathway. Am J Cancer Res. 2018; 8(3): 422–434.PMCID: PMC5883093
    Species: Human
    Sample type: Conditioned Media
  5. Chien YC, Liu LC, Ye HY, Wu JY, Yu YL. EZH2 promotes migration and invasion of triple-negative breast cancer cells via regulating TIMP2-MMP-2/-9 pathway. Am J Cancer Res. 2018 Mar 1;8(3):422-434. PMID: 29636998; PMCID: PMC5883093.
    Species: Human
    Sample type: Conditioned media
  6. Perrigue P., Silva M., Warden C., et al. The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines. Mol Cancer Res. 2015 Apr;13(4):636-50. doi: 10.1158/1541-7786.MCR-13-0268
    Species: Human
    Sample type: Conditioned Media
  7. Salhi A., Farhadian J., Giles K., et al. RSK1 Activation Promotes Invasion in Nodular Melanoma. Am J Pathol. 2015 Mar;185(3):704-16. doi: 10.1016/j.ajpath.2014.11.021.
    Species: Human
    Sample type: Tissue Lysate
  8. Huang J., Yang B., Xiang T., et al. Diallyl disulfide inhibits growth and metastatic potential of human triple-negative breast cancer cells through inactivation of the ?-catenin signaling pathway. Mol Nutr Food Res. 2015 Jun;59(6):1063-75. doi: 10.1002/mnfr.201400668.
    Species: Human
    Sample type: Conditioned Media
  9. Carduner L., Leroy-Dudal J., Picot CR., Gallet O., Carreiras F., et al. Ascites-induced shift along epithelial-mesenchymal spectrum in ovarian cancer cells: enhancement of their invasive behavior partly dependant on ?v integrins. Clinical and Experimental Metastasis. Jun 2014 Epub Ahead of Print.
    Species: Human
    Sample type: Peritoneal Fluid
  10. Ichikawa T., Sugiura H., Koarai A., Minakata Y., Kikuchi T., Morishita Y., et al. TLR3 Activation Augments Matrix Metalloproteinase Production through Reactive Nitrogen Species Generation in Human Lung Fibroblasts. The Journal of Immunology April 23, 2014 1302919 [Epub Ahead of Print]
    Species: Human
    Sample type: Conditioned Media
2 8 well assays were used for an experiment and it worked well. I liked having the instructions available via web however, I used an old printed out manual that I received previously.

Indiana University School of Dentistry
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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