Human MMP Array C1

RayBio® C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.

$487.83  
AAH-MMP-1-4
+

Assay Format

Pathway:
  • Akt Signaling
  • HIF-1 alpha Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Protein Information

Gene Symbols:
  • MMP1
  • MMP10
  • MMP13
  • MMP2
  • MMP3
  • MMP8
  • MMP9
  • TIMP1
  • TIMP2
  • TIMP4

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
MMP-1
MMP-2
MMP-3
MMP-8
MMP-9
MMP-10
MMP-13
TIMP-1
TIMP-2
TIMP-4

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Orgaz J., et al. Diverse matrix metalloproteinase functionsregulate cancer amoeboid migration. Nature Communications 5, Article number:4255. doi:10.1038/ncomms5255
    Species: Human
    Sample type: Conditioned Media (A375P and A375M2 cell supernate)
  2. Connolly M., Mullan RH., McCormick J., et al. Acute-phase serum amyloid A regulates tumor necrosis factor ? and matrix turnover and predicts disease progression in patients with inflammatory arthritis before and after biologic therapy. Arthritis Rheum. 2012 Apr;64(4):1035-45. doi: 10.1002/art.33455. Epub 2011 Nov 10.
    Species: Human
    Sample type: Conditioned Media (Fibroblast Like Synoviocytes)
  3. Jones, Brian A., et al. "Role of ADAM?17, p38 MAPK, Cathepsins, and the Proteasome Pathway in the Synthesis and Shedding of Fractalkine/CX3CL1 in Rheumatoid Arthritis." Arthritis & Rheumatology 65.11 (2013): 2814-2825.
    Species: Human
    Sample type: Conditioned Media (Rheumatoid arthritis synovial fibroblasts)
  4. Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1693
    Species: Human
    Sample type: Conditioned Media (conditioned or non-conditioned medium)
  5. Smith GN Jr, Mickler EA, Payne KK, Lee J, et al. Lung Transplant Metalloproteinase Levels are Elevated Prior to Bronciolitis Obliterans Syndrome. Am J Transplant. 2007;7:1656-1661.
    Species: Human
    Sample type: BAL Fluid (Bronchoalveolar lavage (BAL))
  6. Thielitz A, Vetter R, Schultze B, Wrenger S, Simeoni L,et al.Inhibitors of Dipeptidyl Peptidase IV-Like Activity Mediate Antifibrotic Effects in Normal and Keloid-Derived Skin Fibroblasts. J Investig Dermatol. 2008;128:855-866
    Species: Human
    Sample type: Conditioned Media
  7. Tinaburri L, Valente C, Teson M, Minafò YA, Cordisco S, Guerra L, Dellambra E. The Secretome of Aged Fibroblasts Promotes EMT-Like Phenotype in Primary Keratinocytes from Elderly Donors through BDNF-TrkB Axis. J Invest Dermatol. 2020 Sep 12:S0022-202X(20)32062-5. doi: 10.1016/j.jid.2020.08.019. Epub ahead of print. PMID: 32931807.
    Species: Human
    Sample type: Conditioned Media
  8. Abdul Muneer P., Alikunju S., Szlachetka A., Haorah J. The mechanisms of cerebral vascular dysfunction and neuroinflammation by MMP-mediated degradation of VEGFR-2 in alcohol ingestion. Arterioscler Thromb Vasc Biol. 2012 May;32(5):1167-77. doi: 10.1161/ATVBAHA.112.247668
    Species: Human
    Sample type: Conditioned Media (Human Brain endothelial cells treated with ethanol)
  9. Cheng YC, Chiang HY, Cheng SJ, Chang HW, Li YJ, Shieh SY. Loss of the tumor suppressor BTG3 drives a pro-angiogenic tumor microenvironment through HIF-1 activation. Cell Death Dis. 2020 Dec 11;11(12):1046. doi: 10.1038/s41419-020-03248-5. PMID: 33311481; PMCID: PMC7732837.
    Species: Mouse
    Sample type: Conditioned media
  10. Del Carlo M, Schwartz D, Erickson EA, Loeser RF. Endogenous production of reactive oxygen species is required for stimulstion of human articular chondrocyte matrix metalloproteinase production by fibronectin fragments. Free Radical Biol Med. 2007;42:1350-1358.
    Species: Human
    Sample type: Conditioned Media (chondrocyte cell-cultured medium)
2 8 well assays were used for an experiment and it worked well. I liked having the instructions available via web however, I used an old printed out manual that I received previously.

Indiana University School of Dentistry
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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