Human/Mouse AKT Pathway Phosphorylation Array C1

RayBio® C-Series Human/Mouse AKT Pathway Phosphorylation Array C1. Detects 18 phosphorylated human and mouse proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.


Antigen Information

Gene Symbols:
AKT1 | AKT1S1 | BAD | CDKN1B | EIF4EBP1 | GSK3A | GSK3B | MAPK1 | MAPK3 | MTOR   View more

Assay Format

  • Akt Signaling
  • AMPK Signaling
  • HER/ErbB Signaling
  • HIF-1 alpha Signaling
  • IGF Signaling
  • JAK/STAT Signaling
  • MAPK Signaling
  • mTOR Signaling
  • PI3K-AKT Signaling
  • TGF-beta Signaling
  • Wnt/beta-Catenin Signaling
Number of Targets Detected:
Species Detected:
  • Human
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Solid Support:

Product Specifications

2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473)
(PKB (P-Ser473))
AMPKa (P-Thr172)
  • Gene Symbol: PRKAA1 AMPK1
  • Gene ID: 5562
  • Accession #: Q13131
BAD (P-Ser112)
4E-BP1 (P-Thr36)
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
GSK3a (P-Ser21)
GSK3b (P-Ser9)
mTOR (P-Ser2448)
p27 (P-Thr198)
P53 (P-Ser15)
P70S6K (P-Thr421/Ser424)
PDK1 (P-Ser241)
  • Gene Symbol: PDK1 PDHK1
  • Gene ID: 5170
  • Accession #: O15530
PRAS40 (P-Thr246)
Raf-1 (Ser301)
RPS6 (P-Ser235/Ser236)
RSK1 (P-Ser380)
RSK2 (P-Ser386)
  • Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2
  • Gene ID: 10432
  • Accession #: P51812

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Typical Data

Figure 1 - NIH3T3 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with PDGFβ for 10 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Figure 2 - 293 cells were grown to 80% confluency and then serum starved 24 hours. Cells were either untreated (bottom panel) or treated (top panel) with Insulin for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Shipped promptly and consistent results! Highly recommend!

Cleveland Clinic
We use this kit to test our AKT inhibitors in human cells. Although there seemed to have a background, overall it worked fine for us.
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Analysis Tool Bundle Discount

Specify Size
1 x $342.26
1 x $116.15 $34.84

70% off the data analysis tool when you purchase the array and tool together!

Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department ([email protected]).

Total list price $458.41
Price for all $377.10


This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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