Human/Mouse AKT Pathway Phosphorylation Array C1

RayBio® C-Series Human/Mouse AKT Pathway Phosphorylation Array C1. Detects 18 phosphorylated human and mouse proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.


Antigen Information

Gene Symbols:
AKT1 | AKT1S1 | BAD | CDKN1B | EIF4EBP1 | GSK3A | GSK3B | MAPK1 | MAPK3 | MTOR   View more

Assay Format

  • Akt Signaling
  • AMPK Signaling
  • HER/ErbB Signaling
  • HIF-1 alpha Signaling
  • IGF Signaling
  • JAK/STAT Signaling
  • MAPK Signaling
  • mTOR Signaling
  • PI3K-AKT Signaling
  • TGF-beta Signaling
  • Wnt/beta-Catenin Signaling
Number of Targets Detected:
Species Detected:
  • Human
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473)
(PKB (P-Ser473))
AMPKa (P-Thr172)
  • Gene Symbol: PRKAA1 AMPK1
  • Gene ID: 5562
  • Accession #: Q13131
BAD (P-Ser112)
4E-BP1 (P-Thr36)
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
GSK3a (P-Ser21)
GSK3b (P-Ser9)
mTOR (P-Ser2448)
p27 (P-Thr198)
P53 (P-Ser15)
P70S6K (P-Thr421/Ser424)
PDK1 (P-Ser241)
  • Gene Symbol: PDK1 PDHK1
  • Gene ID: 5170
  • Accession #: O15530
PRAS40 (P-Thr246)
Raf-1 (Ser301)
RPS6 (P-Ser235/Ser236)
RSK1 (P-Ser380)
RSK2 (P-Ser386)
  • Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2
  • Gene ID: 10432
  • Accession #: P51812

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Typical Data

Figure 1 - NIH3T3 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with PDGFβ for 10 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Figure 2 - 293 cells were grown to 80% confluency and then serum starved 24 hours. Cells were either untreated (bottom panel) or treated (top panel) with Insulin for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Yang Y, Wang J, Guo S, et al. Non-lethal sonodynamic therapy facilitates the M1-to-M2 transition in advanced atherosclerotic plaques via activating the ROS-AMPK-mTORC1-autophagy pathway. Redox Biol. 2020;32:101501. doi:10.1016/j.redox.2020.101501
    Species: Mouse
    Sample type: Cell culture
  2. Cai S, Yang Q, Cao Y, et al. PF4 antagonizes retinal neovascularization via inhibiting PRAS40 phosphorylation in a mouse model of oxygen-induced retinopathy. Biochim Biophys Acta Mol Basis Dis. 2020;1866(3):165604. doi:10.1016/j.bbadis.2019.165604
    Species: Mouse
    Sample type: Human vitreous fluid
  3. Qiao L, Hu S, Liu S, et al. microRNA-21-5p dysregulation in exosomes derived from heart failure patients impairs regenerative potential.?J Clin Invest. 2019;129(6):2237-2250. Published 2019 Apr 29. doi:10.1172/JCI123135
    Species: Human
    Sample type: Cell Lysates
  4. Li M, Yang J, Zhang L, et al. A low-molecular-weight compound exerts anticancer activity against breast and lung cancers by disrupting EGFR/Eps8 complex formation.?J Exp Clin Cancer Res. 2019;38(1):211. Published 2019 May 22. doi:10.1186/s13046-019-1207-y
    Species: Human
    Sample type: Cell Lysates
  5. Sustova, Hana, et al. "Opposing effects of 25?hydroxy?and 1?, 25?dihydroxy?vitamin D3 on pro?cachectic cytokine?and cancer conditioned medium?induced atrophy in C2C12 myotubes."?Acta Physiologica?226.3 (2019): e13269.
    Species: Mouse
    Sample type: Cell Lysates
  6. Lu J, Liu QH, Wang F, et al. Exosomal miR-9 inhibits angiogenesis by targeting MDK and regulating PDK/AKT pathway in nasopharyngeal carcinoma.?J Exp Clin Cancer Res. 2018;37(1):147. Published 2018 Jul 13. doi:10.1186/s13046-018-0814-3
    Species: Human
    Sample type: Cell lysates
  7. Chiurchiu V., Rapino C., Talamonti E., et al. Anandamide Suppresses Proinflammatory T Cell Responses In Vitro through Type-1 Cannabinoid Receptor-Mediated mTOR Inhibition in Human Keratinocytes. J Immunol. 2016 Nov 1;197(9):3545-3553. doi: 10.4049/jimmunol.1500546
    Species: Human
    Sample type: Cell Lysate
  8. Chiurchiù, Valerio, et al. "Anandamide suppresses proinflammatory T cell responses in vitro through type-1 cannabinoid receptor?mediated mTOR inhibition in human keratinocytes."?The Journal of Immunology?197.9 (2016): 3545-3553.
    Species: Human
    Sample type: Cell Lysates

No posts found

Write A Review
U.S. Non-U.S.


This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

C-Series Testing Service C-Series Testing Service

Related Products Related Products