Pathway:
- Akt Signaling
- AMPK Signaling
- HER/ErbB Signaling
- HIF-1 alpha Signaling
- IGF Signaling
- JAK/STAT Signaling
- MAPK Signaling
- mTOR Signaling
- PI3K-AKT Signaling
- TGF-beta Signaling
- Wnt/beta-Catenin Signaling
Number of Targets Detected:
18
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Size:
2, 4, or 8 Sample Kit
Gene Symbols:
AKT1 | AKT1S1 | BAD | CDKN1B | EIF4EBP1 | GSK3A | GSK3B | MAPK1 | MAPK3 | MTOR
| PDK1 | PRKAA1 | PTEN | RAF1 | RPS6 | RPS6KA1 | RPS6KA3 | RPS6KB1 | TP53  View more  Hide
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Scroll over each target protein for more information |
Akt (P-Ser473) (PKB (P-Ser473))
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AMPKa (P-Thr172)
- Gene Symbol: PRKAA1 AMPK1
- Gene ID: 5562
- Accession #: Q13131
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ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
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PDK1 (P-Ser241)
- Gene Symbol: PDK1 PDHK1
- Gene ID: 5170
- Accession #: O15530
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RSK2 (P-Ser386)
- Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2
- Gene ID: 10432
- Accession #: P51812
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Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Figure 1 - NIH3T3 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with PDGFβ for 10 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Figure 2 - 293 cells were grown to 80% confluency and then serum starved 24 hours. Cells were either untreated (bottom panel) or treated (top panel) with Insulin for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.