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Home/Search results/Products/Arrays/Antibody Arrays/C-Series/AKT Arrays/Human/Mouse AKT Pathway Phosphorylation Array C1

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Antigen Information

Gene Symbols:

Assay Format

Pathway:

Akt Signaling
AMPK Signaling
HER/ErbB Signaling
HIF-1 alpha Signaling
IGF Signaling
JAK/STAT Signaling
MAPK Signaling
mTOR Signaling
PI3K-AKT Signaling
TGF-beta Signaling
Wnt/beta-Catenin Signaling

Number of Targets Detected:

Species Detected:

Human
Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473) (PKB (P-Ser473)) Gene Symbol: AKT1 PKB RAC Gene ID: 207 Accession #: P31749 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA	AMPKa (P-Thr172) Gene Symbol: PRKAA1 AMPK1 Gene ID: 5562 Accession #: Q13131	BAD (P-Ser112) Gene Symbol: BAD BBC6 BCL2L8 Gene ID: 572 Accession #: Q92934 ELISA Kit: RayBio Human Phospho-BAD (S112) ELISA	4E-BP1 (P-Thr36) Gene Symbol: EIF4EBP1 Gene ID: 1978 Accession #: Q13541 ELISA Kit: RayBio Human/Mouse Phospho-4E-BP1 (T36) ELISA	ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187) Gene Symbol: MAPK1 ERK2 PRKM1 PRKM2 Gene ID: 5594 Accession #: P28482 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (T185/Y187) and Total Erk1/2 ELISA
GSK3a (P-Ser21) Gene Symbol: GSK3A Gene ID: 2931 Accession #: P49840 ELISA Kit: RayBio Human/Mouse/Rat Phospho-GSK3a (S21) ELISA	GSK3b (P-Ser9) Gene Symbol: GSK3B Gene ID: 2932 Accession #: P49841 ELISA Kit: RayBio Human Phospho-GSK3b (S9) ELISA	mTOR (P-Ser2448) Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1 Gene ID: 2475 Accession #: P42345 ELISA Kit: RayBio Human Phospho-mTOR (S2448) ELISA	p27 (P-Thr198) Gene Symbol: PSMD9 Gene ID: 5715 Accession #: O00233 ELISA Kit: RayBio Human Phospho-P27/Kip1 (T198) ELISA	P53 (P-Ser15) Gene Symbol: TP53 P53 Gene ID: 6198 Accession #: P23443 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA
P70S6K (P-Thr421/Ser424) Gene Symbol: RPS6KB1 STK14A Gene ID: 6198 Accession #: P23443 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P70S6K (T421/S424) ELISA	PDK1 (P-Ser241) Gene Symbol: PDK1 PDHK1 Gene ID: 5170 Accession #: O15530	PRAS40 (P-Thr246) Gene Symbol: AKT1S1 PRAS40 Gene ID: 84335 Accession #: Q96B36 ELISA Kit: RayBio Human Phospho-PRAS40 (T246) ELISA	PTEN (P-Ser380) Accession #: P60484; O08586; O54857 ELISA Kit: RayBio Human/Mouse/Rat Phospho-PTEN (S380) ELISA	Raf-1 (Ser301) Gene Symbol: RAF1 RAF Gene ID: 5894 Accession #: P04049 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Raf-1 (Ser301) ELISA
RPS6 (P-Ser235/Ser236) Gene Symbol: RPS6 OK/SW-cl.2 Gene ID: 6194 Accession #: P62753 ELISA Kit: RayBio Human/Mouse/Rat Phospho-RPS6 (S235/S236) ELISA	RSK1 (P-Ser380) Gene Symbol: RPS6KA1 MAPKAPK1A RSK1 Gene ID: 6195 Accession #: Q15418 ELISA Kit: RayBio Human Phosph-RSK1 (S380) ELISA	RSK2 (P-Ser386) Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2 Gene ID: 10432 Accession #: P51812

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
1,000X HRP-Anti-Rabbit IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Typical Data

Figure 1 - NIH3T3 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with PDGFβ for 10 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Figure 2 - 293 cells were grown to 80% confluency and then serum starved 24 hours. Cells were either untreated (bottom panel) or treated (top panel) with Insulin for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.