RayBio® Human NAP-2 (PPBP/CXCL7) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
8.5 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
1,000 - 20,000 fold
8.5 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P02775
Gene ID:
  • 5473
Gene Symbols:
  • CTAP3
  • CXCL7
  • PPBP
  • SCYB7
  • TGB1
  • THBGB1
Protein Name & Synonyms:
Platelet basic protein (PBP) (C-X-C motif chemokine 7) (Leukocyte-derived growth factor) (LDGF) (Macrophage-derived growth factor) (MDGF) (Small-inducible cytokine B7) [Cleaved into: Connective tissue-activating peptide III (CTAP-III) (LA-PF4) (Low-affinity platelet factor IV) TC-2 Connective tissue-activating peptide III(1-81) (CTAP-III(1-81)) Beta-thromboglobulin (Beta-TG) Neutrophil-activating peptide 2(74) (NAP-2(74)) Neutrophil-activating peptide 2(73) (NAP-2(73)) Neutrophil-activating peptide 2 (NAP-2) TC-1 Neutrophil-activating peptide 2(1-66) (NAP-2(1-66)) Neutrophil-activating peptide 2(1-63) (NAP-2(1-63))]
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Chen H., Shi B., Feng X., et al. Leptin and Neutrophil-Activating Peptide 2 Promote Mesenchymal Stem Cell Senescence Through Activation of the Phosphatidylinositol 3-Kinase/Akt Pathway in Patients With Systemic Lupus Erythematosus. Arthritis Rheumatol. 2015 Sep;67(9):2383-93. doi: 10.1002/art.39196.
    Species: Human
    Sample type: Serum (MSC senescence in Systemic Lupus Erthematosus (SLE))
  2. Blankley, Richard T., et al. "A label-free selected reaction monitoring workflow identifies a subset of pregnancy specific glycoproteins as potential predictive markers of early-onset pre-eclampsia." Molecular & Cellular Proteomics 12.11 (2013): 3148-3159.
    Species: Human
    Sample type: Plasma (early pregnancy - Early-onset Pre-eclampsia identification )
  3. Dutta A, Ray MR, Mukherjee B, Chowdhury S. Increased cardiovascular risk in association with chronic airflow obstruction among premenopausal rural women of India who cook exclusively with biomass. Air Qual Atmos Health. 2013;6(1):307-315.
    Species: Human
    Sample type: Serum
  4. Ustünda?, M., et al. "Comparative diagnostic accuracy of serum levels of neutrophil activating peptide-2 and pentraxin-3 versus troponin-I in acute coronary syndrome." Anadolu kardiyoloji dergisi: AKD= the Anatolian journal of cardiology 11.7 (2011): 588.
    Species: Human
    Sample type: Serum (Acute coronary syndrome patients)
  5. Kokcam I., Turgut D., Ilhan NF., Cick D. The levels of serum chemokines in patients with Behçet's disease. Turk J Med Sci 2012; 42 (6): 1105-1110. doi:10.3906/sag-1010-1220
    Species: Human
    Sample type: Serum (Behçet’s disease patients)

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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