Human NF-κB Pathway Phosphorylation Array C1

RayBio® C-Series Human NF-kB Pathway Phosphorylation Array 1 Kit. Detects 11 phosphorylated proteins in human samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

AAH-NFKB-1-2
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Antigen Information

Gene Symbols:
ATM | EIF2S1 | HDAC2 | HDAC4 | MAP3K7 | NFKBIA | RELA | RPS6KA5 | STAT1 | TBK1   View more

Assay Format

Pathway:
  • MAPK Signaling
  • mTOR Signaling
  • NFkB Signaling
  • p53 Signaling
  • PKC Signaling
Number of Targets Detected:
11
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ATM (P-Ser1981)
eIF-2a (P-Ser52)
HDAC2 (P-Ser394)
HDAC4 (P-Ser632)
IKB-alpha (P-Ser32)
MSK1 (P-Ser376)
NF-kB (P-Ser536)
STAT1 (P-Ser727)
TAK1 (P-Ser412)
TBK1 (P-Ser172)
ZAP70 (P-Tyr292)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human NF-kB Pathway Phosphorylation Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or plastic Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Typical Data

Figure 1 - HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with TNF alpha and Calyculin A. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Note the strong signals of the Positive Control spots in the upper left and lower right corners. (See below for further details on the control spots.)

The signal intensity for each antigen-specific antibody spot is proportional to the relative concentration of the antigen in that sample. Comparison of signal intensities for individual antigen-specific antibody spots between and among array images can be used to determine relative differences in expression levels of each analyte sample-to-sample or group-to-group.


Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
We used it to detect the NF-KB pathway phosphorylation, the background is acceptable, works fine.
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Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department ([email protected]).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.