Human Osteoprotegerin (OPG) ELISA

RayBio® Human Osteoprotegerin (TNFRSF11B) ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • O00300
Gene ID:
  • 4982
Gene Symbols:
  • OCIF
  • OPG
Protein Name & Synonyms:
Tumor necrosis factor receptor superfamily member 11B (Osteoclastogenesis inhibitory factor) (Osteoprotegerin)
Target Species:
  • Human

Assay Format

Detection Range:
1 pg/ml - 900 pg/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
1 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Jiang, Weidong, et al. "Identification of five serum protein markers for detection of ovarian cancer by antibody arrays."?PloS one?8.10 (2013): e76795.
    Species: Human
    Sample type: Serum
  2. Kamel, Maher A., et al. "Insulin-like growth factor-1 and osteocalcin are correlated with markers of osteoporosis in postmenopausal women with type-2 diabetes."?Open Journal of Endocrine and Metabolic Diseases?3.05 (2013): 245.
    Species: Human
    Sample type: Serum
  3. Prieto Damm, D., et al. "Relación entre los niveles de compuestos volátiles sulfurados con los niveles de RANKL y OPG en pacientes con periodontitis crónica moderada o severa: Estudio transversal."?Revista clínica de periodoncia, implantología y rehabilitación oral?6.1 (2013): 20-24.
    Species: Human
    Sample type: Gingival crevicular fluid
  4. Chaudhari, Amol, et al. "Modified titanium surface-mediated effects on human bone marrow stromal cell response."?Materials?6.12 (2013): 5533-5548.
    Species: Human
    Sample type: Conditioned Media
  5. Aihara, Norihito, et al. "Levels of IL-17, RANKL and OPG in Gingival Crevicular Fluid Obtained from Patients with Severe Orthodontic Root Resorption."?International Journal of Oral-Medical Sciences?12.1 (2013): 54-60.
    Species: Human
    Sample type: Gingival Crevicular Fluid
  6. Erkol A., Oduncu V., Pala S., et al. Plasma osteoprotegerin level on admission is associated with no-reflow phenomenon after primary angioplasty and subsequent left ventricular remodeling in patients with acute ST-segment elevation myocardial infarction. Atherosclerosis. 2012 Mar;221(1):254-9. doi: 10.1016/j.atherosclerosis.2011.12.031.
    Species: Human
    Sample type: Plasma
  7. Zheng C., Chu P., Wu C., et al. Association between increased serum osteoprotegerin levels and improvement in bone mineral density after parathyroidectomy in hemodialysis patients. Tohoku J Exp Med. 2012;226(1):19-27.
    Species: Human
    Sample type: Serum
  8. Attia, Enas AS, et al. "Assessment of osteoporosis in psoriasis with and without arthritis: correlation with disease severity."?International journal of dermatology?50.1 (2011): 30-35.
    Species: Human
    Sample type: Serum
  9. Tang, T., et al. "Effect of water-soluble P-chitosan and S-chitosan on human primary osteoblasts and giant cell tumor of bone stromal cells."?Biomedical Materials?6.1 (2011): 015004.
    Species: Human
    Sample type: Conditioned Media
  10. Erkol, Ayhan, et al. "Relation of circulating osteoprotegerin levels on admission to microvascular obstruction after primary percutaneous coronary intervention."?The American journal of cardiology?107.6 (2011): 857-862.
    Species: Human
    Sample type: Plasma

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