Human Osteoprotegerin (OPG) ELISA

RayBio® Human Osteoprotegerin (TNFRSF11B) ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • O00300
Gene ID:
  • 4982
Gene Symbols:
  • OCIF
  • OPG
Protein Name & Synonyms:
Tumor necrosis factor receptor superfamily member 11B (Osteoclastogenesis inhibitory factor) (Osteoprotegerin)
Target Species:
  • Human

Assay Format

Detection Range:
1 pg/ml - 900 pg/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
1 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Abdallah E., El-Shishtawy S., Sherif N., Abdelwahab M. Diagnostic Performance of Urinary Osteoprotegrin as A Novel Biomarker for Early Detection of Lupus Nephritis Activity. Life Science Journal 2015;12(1) 75-81]. (ISSN:1097-8135)
    Species: Human
    Sample type: Urine
  2. Wang J., Sun X., Zhang H., Wang Y., Li Y. MPA influences tumor cell proliferation, migration, and invasion induced by RANKL through PRB involving the MAPK pathway in endometrial cancer. Oncol Rep. 2015 Feb;33(2):799-809. doi: 10.3892/or.2014.3651
    Species: Human
    Sample type: Serum
  3. KOÃ?Y???T I.; TÃ?RKMEN K; DO?AN E., et al. The Relationship Between Osteoprotegerin/RANKL Axis and Arterial Stiffness in Osteopenic/Osteoporotic Renal Transplantation Recipients. Turk Neph Dial Transpl 2015; 24 (2): 181-188 doi: 10.5262/tndt.2015.1002.07
    Species: Human
    Sample type: Serum
  4. Bakry R., El-Sayed M., Hamza H., Hassan K. Pretreatment levels of serum osteoprotegerin and p53 protein and urine telomerase as prognostic factors affecting survival in Egyptian bladder cancer patients. Oncol Lett. 2016 Jan;11(1):823-830. Epub 2015 Nov 17.
    Species: Human
    Sample type: Serum
  5. Kapelouzou A., Tsourelis L., Kaklamanis L., et al. Serum and tissue biomarkers in aortic stenosis. Glob Cardiol Sci Pract. 2015 Nov 13;2015(4):49. doi: 10.5339/gcsp.2015.49
    Species: Human
    Sample type: Serum
  6. Alan R., Marakoglu I., Haliloglu S. Peri-implant crevicular fluid levels of cathepsin-K, RANKL, and OPG around standard, short, and mini dental implants after prosthodontic loading. J Periodontal Implant Sci. 2015 Oct; 45(5): 169-177. doi: 10.5051/jpis.2015.45.5.169
    Species: Human
    Sample type: Gingival Crevicular Fluid
  7. Filis K., Martinakis V., Galyfos G., Sigala F., Theodorou D., Andreadou I., Zografos G. OSTEOPONTIN AND OSTEOPROTEGERIN AS POTENTIAL BIOMARKERS IN ABDOMINAL AORTIC ANEURYSM BEFORE AND AFTER TREATMENT. International Scholarly Research Notices, 2014 May, Epub Ahead of Print
    Species: Human
    Sample type: Serum
  8. Holt V, Caplan AI, Haynesworth SE (2014) Identification of a Subpopulation of Marrow MSC-Derived Medullary Adipocytes That Express Osteoclast- Regulating Molecules: Marrow Adipocytes Express Osteoclast Mediators. PLoS ONE 9(10): e108920. doi:10.1371/journal.pone.0108920
    Species: Human
    Sample type: Conditioned Media
  9. Lucas R., Ramos E., Prata M., Rodrigues A., Costa L., et al. Changes in serum RANKL and OPG with sexual development and their associations with bone turnover and bone mineral density in a cohort of girls. Clinical Biochemistry Available online 22 April 2014 [Epub ahead of Print]
    Species: Human
    Sample type: Serum
  10. Scher J., et al. Decreased Bacterial Diversity Characterizes an Altered Gut Microbiota in Psoriatic Arthritis and Resembles Dysbiosis of Inflammatory Bowel Disease. Arthritis and Rhuematology November 2014, epub ahead of print. DOI 10.1002/art.38892
    Species: Human
    Sample type: Stool

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