Human Osteoprotegerin (OPG) ELISA

RayBio® Human Osteoprotegerin (TNFRSF11B) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$756.00  
ELH-OPG-2
+

Assay Format

Detection Range:
1 pg/ml - 900 pg/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • O00300
Gene ID:
  • 4982
Gene Symbols:
  • OCIF
  • OPG
  • TNFRSF11B
Protein Name & Synonyms:
Tumor necrosis factor receptor superfamily member 11B (Osteoclastogenesis inhibitory factor) (Osteoprotegerin)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Maksymowych WP, Landewe R, Conner-Spady B, Dougados, et al. Serum Matrix Metalloproteinase 3 Is an Independent Predictor of Structural Damage Progression in Patients with Ankylosing Spondylitis. Arthritis Rheum. 2007;56(6):1846-1853.
    Species: Human
    Sample type: Serum (Human serum)
  2. Scher J., et al. Decreased Bacterial Diversity Characterizes an Altered Gut Microbiota in Psoriatic Arthritis and Resembles Dysbiosis of Inflammatory Bowel Disease. Arthritis and Rhuematology November 2014, epub ahead of print. DOI 10.1002/art.38892
    Species: Human
    Sample type: Stool (Fecal material)
  3. Mohamed JA, DuPont HL, Jiang ZD, Flores J, Carlin LG, Belkind-Gerson J, et al. A Single-Nucleotide Polymorphism in the Gene Encoding Osteoprotegerin, an Anti-inflammatory Protein Produced in Response to Infection with Diarrheagenic Escherichia coli, Is Associated with an Increased Risk of Nonsecretory Bacterial Diarrhea in North American Travelers to Mexico. J Infect Dis. 2009;199:477-485.
    Species: Human
    Sample type: Conditioned Media (T84 cell culture sups)
  4. Mohamed JA, DuPont HL, Jiang ZD, Flores J, Carlin LG, Belkind-Gerson J, et al. A Single-Nucleotide Polymorphism in the Gene Encoding Osteoprotegerin, an Anti-inflammatory Protein Produced in Response to Infection with Diarrheagenic Escherichia coli, Is Associated with an Increased Risk of Nonsecretory Bacterial Diarrhea in North American Travelers to Mexico. J Infect Dis. 2009;199:477-485.
    Species: Human
    Sample type: Stool (Stool samples )
  5. MacMillan AK., Lamberti FV., Moulton JN., et al. Similar healthy osteoclast and osteoblast activity on nanocrystalline hydroxyapatite and nanoparticles of tri-calcium phosphate compared to natural bone. Int J Nanomedicine. 2014; 9: 5627-5637.
    Species: Human
    Sample type: Nanomaterials (Nanomaterials vs Bone)
  6. MacMillan AK., Lamberti FV., Moulton JN., et al. Similar healthy osteoclast and osteoblast activity on nanocrystalline hydroxyapatite and nanoparticles of tri-calcium phosphate compared to natural bone. Int J Nanomedicine. 2014; 9: 5627-5637.
    Species: Human
    Sample type: Tissue Lysate (Nanomaterials vs Bone)
  7. Fuernau, Georg, et al. "Osteoprotegerin in ST-elevation myocardial infarction: prognostic impact and association with markers of myocardial damage by magnetic resonance imaging." International journal of cardiology 167.5 (2013): 2134-2139.
    Species: Human
    Sample type: Serum (patients with acute STEMI )
  8. Erkol A., Oduncu V., Pala S., et al. Plasma osteoprotegerin level on admission is associated with no-reflow phenomenon after primary angioplasty and subsequent left ventricular remodeling in patients with acute ST-segment elevation myocardial infarction. Atherosclerosis. 2012 Mar;221(1):254-9. doi: 10.1016/j.atherosclerosis.2011.12.031.
    Species: Human
    Sample type: Plasma (Myocardial Infaction patients)
  9. Palumbo S, Li W-J. Osteoprotegerin Enhances Osteogenesis of Human Mesenchymal Stem Cells. Tissue Eng Part A. Volume 19, Numbers 19 and 20, 2013, DOI: 10.1089/ten.tea.2012.0550
    Species: Human
    Sample type: Conditioned Media (MG-63 cells and hMSC Conditioned Medias.)
  10. Jose´ I Botella-Carretero, Francisco Alvarez-Blasco, Jose´ Luis San Milla´n and He´ctor F Escobar-Morreale. Thyroid hormone deficiency and postmenopausal status independently increase serum osteoprotegerin concentrations in women. Eur J Endocrinol. 2007;156:539-545
    Species: Human
    Sample type: Serum (Serum)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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