Protein Name & Synonyms:
Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53)
Western blot: 1-2ug/ml
IHC (FFPE): 1-2ug/ml for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Human recombinant protein was used as the immunogen for this p53 antibody.
Protein G affinity chromatography
Human. Other species not known.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons. [RefSeq]
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.