RayBio® Human PDGF-BB ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.


Assay Format

Detection Range:
1 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma):
5 - 50 fold
1 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P01127
Gene ID:
  • 5155
Gene Symbols:
  • PDGF2
  • SIS
Protein Name & Synonyms:
Platelet-derived growth factor subunit B (PDGF subunit B) (PDGF-2) (Platelet-derived growth factor B chain) (Platelet-derived growth factor beta polypeptide) (Proto-oncogene c-Sis) (Becaplermin)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Cadamuro M, Nardo G, Indraccolo S, et al. Platelet-derived growth factor-D and Rho GTPases regulate recruitment of cancer-associated fibroblasts in cholangiocarcinoma. Hepatology. 2013 Sep;58(3):1042-1053.
    Species: Human
    Sample type: Conditioned Media (CCA (cholangiocarcinoma) cells and controls)
  2. Li Y, Zhang C,Li B, Zhao L, Li X,et al.Ultrasensitive Densitometry Detection of Cytokines with Nanoparticle-Modified Aptamers.Clin Chem.2007;53(6):1061-1066.
    Species: Human
    Sample type: Serum (Serum )
  3. Li Y, Zhang C, Li B, Zhao L, Li X,et al.Ultrasensitive Densitometry Detection of Cytokines with Nanoparticle-Modified Aptamers.Clin Chem. 2007;53(6):1061-1066.
    Species: Human
    Sample type: Serum (serum)
  4. Li YY, Zhang C, Li BS, Zhao LF, et al. Ultrasensitive Densitometry Detection of Cytokines with Nanoparticle-Modified Aptamers. Clin Chem. 2007;53:1061-1066,
    Species: Human
    Sample type: Serum (Human serum)
  5. El Backly, Rania M., et al. "A platelet-rich plasma-based membrane as a periosteal substitute with enhanced osteogenic and angiogenic properties: a new concept for bone repair." Tissue Engineering Part A 19.1-2 (2012): 152-165.
    Species: Human
    Sample type: Conditioned Media (BMSCs)
  6. Muraglia A., Todeschi M., Papait A., et al. Combined platelet and plasma derivatives enhance proliferation of stem/progenitor cells maintaining their differentiation potential. Cytotherapy. 2015 Dec;17(12):1793-806. doi: 10.1016/j.jcyt.2015.09.004.
    Species: Human
    Sample type: Conditioned Media (Human BM Stromal Cells)
  7. Miyahara T., Nyan M., Shimoda A., et al. Exploitation of a novel polysaccharide nanogel cross-linking membrane for guided bone regeneration (GBR). J Tissue Eng Regen Med. 2012 Aug;6(8):666-72. doi: 10.1002/term.475.
    Species: Human
    Sample type: Serum (Guided Bone regeneration)
  8. Winsz-Szczotka K., et al. Circulating keratan sulfate as a marker of metabolic changes of cartilage proteoglycan in juvenile idiopathic arthritis; influence of growth factors as well as proteolytic and prooxidative agents on aggrecan alterations. Clin Chem Lab Med 2014; aop DOI 10.1515/cclm-2014-0441
    Species: Human
    Sample type: Plasma
  9. Adrych K., Stojek M., Smoczynski M., et al. Increased serum chemerin concentration in patients with chronic pancreatitis. Dig Liver Dis. 2012 May;44(5):393-7. doi: 10.1016/j.dld.2011.06.020.
    Species: Human
    Sample type: Serum (Diabetic Patient samples)
  10. Lima AC., et al. Fast and Mild Strategy, Using Superhydrophobic Surfaces, to Produce Collagen/Platelet Lysate Gel Beads for Skin Regeneration. Stem Cell Rev and Rep, Aug 2014. epub ahead of print. DOI 10.1007/s12015-014-9548-6
    Species: Human
    Sample type: Conditioned Media
I used the kit with the cell culture supernatant. Easy to follow and discuss everything in details

Florida A&M University
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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