Human PF-4 ELISA

RayBio® Human Platelet Factor 4 (CXCL4) ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • P02776
Gene ID:
  • 5196
Gene Symbols:
  • CXCL4
  • PF4
  • SCYB4
Protein Name & Synonyms:
Platelet factor 4 (PF-4) (C-X-C motif chemokine 4) (Iroplact) (Oncostatin-A) [Cleaved into: Platelet factor 4, short form]
Target Species:
  • Human

Assay Format

Detection Range:
20 pg/ml - 15000 pg/ml
Recommended Dilution (serum & plasma):
200 - 2,000 fold
20 pg/ml

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This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Hufner K., Koudouovoh-Tripp P., Kandler C., et al. Differential changes in platelet reactivity induced by acute physical compared to persistent mental stress. Physiol Behav. 2015 Nov 1;151:284-91. doi: 10.1016/j.physbeh.2015.07.021.
    Species: Human
    Sample type: Platelet Releasate
  2. Spaks A., Jaunalksne I., Spaka I., et al. Diagnostic Value of Circulating CXC Chemokines in Non-small Cell Lung Cancer. Anticancer Res. 2015 Dec;35(12):6979-83.
    Species: Human
    Sample type: Serum
  3. Leszczak V., et al. Improved in Vitro Blood Compatibility of Polycaprolactone Nanowire Surfaces. ACS Applied Materials and Interfaces. ASAP ArticleSeptember 3, 2014. DOI: 10.1021/am503508r
    Species: Human
    Sample type: Plasma
  4. Ollivier V., et al. Collagen Can Selectively Trigger a Platelet Secretory Phenotype via Glycoprotein VI. PLOS One Published: August 12, 2014, DOI: 10.1371/journal.pone.0104712
    Species: Human
    Sample type: Platelet Releasate
  5. Popescu et al.: Potential serum biomarkers forglioblastoma diagnostic assessed by proteomic approaches. ProteomeScience 2014 12:47.
    Species: Human
    Sample type: Serum
  6. Thasneem YM, Sajeesh S, Sharma CP. Glucosylated polymeric nanoparticles: A sweetened approach against blood compatibility paradox. Colloids Surf B Biointerfaces. 2013 Aug 1;108:337-344.
    Species: Human
    Sample type: Plasma
  7. Tillack, Kati, et al. "Gender differences in circulating levels of neutrophil extracellular traps in serum of multiple sclerosis patients."?Journal of neuroimmunology?261.1 (2013): 108-119.
    Species: Human
    Sample type: Serum
  8. Thasneem, Y. M., et al. "Biomimetic mucin modified PLGA nanoparticles for enhanced blood compatibility."?Journal of colloid and interface science?409 (2013): 237-244.
    Species: Human
    Sample type: Platelet Rich Plasma
  9. Zabini D, Nagaraj C, Stacher E, Lang IM, Nierlich P, et al. (2012) Angiostatic Factors in the Pulmonary Endarterectomy Material from Chronic Thromboembolic Pulmonary Hypertension Patients Cause Endothelial Dysfunction. PLoS ONE 7(8): e43793. doi:10.1371/journal.pone.0043793
    Species: Human
    Sample type: Conditioned Media
  10. Aneja, Kawalpreet, et al. "Mechanism of platelet factor 4 (PF4) deficiency with RUNX1 haplodeficiency: RUNX1 is a transcriptional regulator of PF4."?Journal of Thrombosis and Haemostasis?9.2 (2011): 383-391.
    Species: Human
    Sample type: Cell Lysate

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