Human RANTES ELISA

RayBio® Human RANTES (CCL5) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$1,608.00  
ELH-RANTES-5
+

Assay Format

Detection Range:
3 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 - 50 fold
Sensitivity:
3 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P13501
Gene ID:
  • 6352
Gene Symbols:
  • CCL5
  • D17S136E
  • SCYA5
Protein Name & Synonyms:
C-C motif chemokine 5 (EoCP) (Eosinophil chemotactic cytokine) (SIS-delta) (Small-inducible cytokine A5) (T cell-specific protein P228) (TCP228) (T-cell-specific protein RANTES) [Cleaved into: RANTES(3-68) RANTES(4-68)]
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Israelow B., Narbus C., Sourisseau M., Evans M. HepG2 cells mount an effective antiviral interferon-lambda based innate immune response to hepatitis C virus infection. Hepatology Epub Ahead of Print, doi: 10.1002/hep.27227
    Species: Human
    Sample type: Conditioned Media (Human 293T, Huh7, Huh7.5, HepG2, and MDCK-NS1 cells)
  2. Vaniotis, G., Rayes, R. F., Qi, S., Milette, S., Wang, N., Perrino, S., … Brodt, P. (2018). Collagen IV-conveyed signals can regulate chemokine production and promote liver metastasis. Oncogene, 37(28), 3790–3805. doi:10.1038/s41388-018-0242-z 
    Species: Human
    Sample type: Conditioned media (cell culture media)
  3. Shangguan L., Ti X., Krause U., et al. Inhibition of TGF-?/Smad signaling by BAMBI blocks differentiation of human mesenchymal stem cells to carcinoma-associated fibroblasts and abolishes their protumor effects. Stem Cells. 2012 Dec;30(12):2810-9. doi: 10.1002/stem.1251.
    Species: Human
    Sample type: Conditioned Media (Mesenchymal Stem Cells)
  4. Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
    Species: Human
    Sample type: Conditioned Media (HIV innoculated brain periocytes)
  5. Zhou R., Wang X., Liu H., et al. GalNAc-Specific Soybean Lectin Inhibits HIV Infection of Macrophages through Induction of Antiviral Factors. J Virol. 2018 Feb 26;92(6). pii: e01720-17. doi: 10.1128/JVI.01720-17.
    Species: Human
    Sample type: Conditioned Media (Human macrophages from PBMCs innovulated with HIV and GalNac lectin)
  6. Zemskova M., et al. Regulation of prostate stromal fibroblasts by the PIM1 protein kinase. Cellular Signalling 27 (2015) 135-146. DOI:10.1016/j.cellsig.2014.10.010
    Species: Human
    Sample type: Conditioned Media (Stromal fibroblasts)
  7. Maussang, David, et al. "Llama-derived single variable domains (nanobodies) directed against chemokine receptor CXCR7 reduce head and neck cancer cell growth in vivo." Journal of Biological Chemistry 288.41 (2013): 29562-29572.
    Species: Human
    Sample type: Conditioned Media (22A cells )
  8. Parnell, Grant P., David R. Booth, and Dorothee Nickles. "The Autoimmune Disease-associated Transcription Factors EOMES and TBX21 Are Dysregulated in Multiple Sclerosis and Define a Molecular Subtype of Disease." Clinical Immunology. ScienceDirect, 15 Jan. 2014. Web.
    Species: Human
    Sample type: Serum (separated from whole blood was collected in a single CAT BD Vacutainer from subjects with )
  9. Pavkova G., Lipkova J., Pavek N., et al. RANTES, MCP-1 chemokines and factors describing rheumatoid arthritis. Mol Immunol. 2012 Oct;52(3-4):273-8. doi: 10.1016/j.molimm.2012.06.006.
    Species: Human
    Sample type: Serum (Fasting biomarkers in Rhuematoid Arthritis)
  10. Ciaffi L., Cavassini M., Genne D., et al. Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study. Eur J Clin Invest. 2015 Jul;45(7):720-30. doi: 10.1111/eci.12464.
    Species: Human
    Sample type: Serum (etravirine treated HIV patients)

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

Recently Viewed Recently Viewed

Customers Also Bought Customers Also Bought