Human Receptor Array C1

RayBio® C-Series Human Receptor Antibody Array 1 Kit. Detects 40 human cytokine receptor proteins. Suitable for all liquid sample types.

$501.97  
AAH-REC-1-4
+

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
AGER | ALCAM | CD14 | CD40LG | CD80 | CEACAM1 | EDA2R | ENG | ERBB3 | FAS   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
ALCAM
(CD166)
CD80
(B7-1)
BCMA
(TNFRSF17)
CD14
CD30
(TNFRSF8)
CD40 Ligand
(TNFSF5)
CEACAM-1
DR6
(TNFRSF21)
Dtk
Endoglin
(CD105)
ErbB3
E-Selectin
Fas
(TNFRSF6/Apo-1)
Flt-3 Ligand
GITR
(TNFRSF18)
HVEM
(TNFRSF14)
ICAM-3
(CD50)
IL-1 R4
(ST2)
IL-1 R1
IL-10 R beta
IL-17 RA
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-21 R
LIMPII
Lipocalin-2
(NGAL)
L-Selectin
(CD62L)
LYVE-1
MICA
MICB
NRG1-beta 1
(HRG1-beta 1)
PDGF R beta
PECAM-1
(CD31)
RAGE
TIM-1
(KIM-1)
TRAIL R3
(TNFRSF10C)
Trappin-2
uPAR
VCAM-1
(CD106)
XEDAR
  • Gene Symbol: EDA2R
  • Gene ID: 60401
  • Accession #: Q9HAV5

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Receptor Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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