Human Resistin ELISA

RayBio® Human Resistin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
2 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma):
20 - 200 fold
2 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q9HD89
Gene ID:
  • 56729
Gene Symbols:
  • FIZZ3
  • HXCP1
  • RETN
  • RSTN
  • UNQ407/PRO1199
Protein Name & Synonyms:
Resistin (Adipose tissue-specific secretory factor) (ADSF) (C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein) (Cysteine-rich secreted protein A12-alpha-like 2) (Cysteine-rich secreted protein FIZZ3)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Nobili V., Alisi A., Cutrera R., et al. Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver. Thorax. 2015 Aug;70(8):769-81. doi: 10.1136/thoraxjnl-2015-206782
    Species: Human
    Sample type: Serum (Biomarkers in pediatric non-alcoholic fatty liver patients)
  2. Kahleova, H., et al. "Vegetarian diet improves insulin resistance and oxidative stress markers more than conventional diet in subjects with Type 2 diabetes." Diabetic Medicine 28.5 (2011): 549-559.
    Species: Human
    Sample type: Plasma
  3. Singh P., et al. Adiponectin-resistin index and its strong association with acute coronary syndrome in South Indian men. Inflammation Research, Sept 2014, epub ahead of print. DOI 10.1007/s00011-014-0771-z
    Species: Human
    Sample type: Serum
  4. Pektas M., Kurt A., Un I., et al. Effects of 17b-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase. Cytokine. 2015 Apr;72(2):130-4. doi: 10.1016/j.cyto.2014.12.020
    Species: Human
    Sample type: Conditioned Media (3T3-L1 or mMSC cells)
  5. Cai D, Xu Y, Ding R, Qiu K, Zhang R, Wang H, Huang L, Xie X, Yan H, Deng Y, Lin X, Shao J, Luo X, Duan C. Extensive serum biomarker analysis in patients with non-small-cell lung carcinoma. Cytokine. 2020 Feb;126:154868. doi: 10.1016/j.cyto.2019.154868. Epub 2019 Oct 16. PMID: 31629110.
    Species: Human
    Sample type: Serum
  6. Kerem M, Ferahkose Z, Yilmaz U, Pasaoglu H, Ofluoglu E, et al. Adipokines and ghrelin in gastric cancer cachexia.World J Gastroenterol. 2008;14(23):3633-3641.
    Species: Human
    Sample type: Serum
  7. Siristatidis C., Askoxylaki M., Varounis C., et al. E-selectin, resistin and reactive oxygen species levels in GnRH -agonist and -antagonist protocols in IVF/ICSI: a prospective cohort study. J Assist Reprod Genet. 2015 Jun;32(6):959-67. doi: 10.1007/s10815-015-0489-z.
    Species: Human
    Sample type: Serum (IVF patients undergoing RnGRH treatments)
  8. Erturk A, Cure E, Parlak E, Cure MC, Yuce S, Kizilkaya B. Serum resistin levels may be new prognostic factor of crimean-congo hemorrhagic fever. International Journal of Clinical and Experimental Medicine 2014;7(10):3536-3542.
    Species: Human
    Sample type: Serum (Crimeran Congo hemorrhagic fever biomarker?)
  9. Erturk A., Cure M., Cure E., et al. Clinical potential of resistin as a novel prognostic biomarker for cellulitis. Exp Ther Med. 2015 May;9(5):1875-1880.
    Species: Human
    Sample type: Serum (Cellulitis patients)
  10. Rashidlamir, Amir, Aliakbar Hashemi Javaheri, and Mohsen Jaafari. "The effect of regular aerobic training with weight loss on concentrations of fibrinogen and resistin in healthy and overweight men." Tehran University Medical Journal 68.12 (2011).
    Species: Human
    Sample type: Serum (aerobic exercise in healthy and overweight middle-aged men)

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