Human Resistin ELISA

$398.00
ELH-Resistin-1

RayBio® Human Resistin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
2 pg/ml

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Detection Range
2 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma)
20 - 200 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Resistin (Adipose tissue-specific secretory factor) (ADSF) (C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein) (Cysteine-rich secreted protein A12-alpha-like 2) (Cysteine-rich secreted protein FIZZ3)
Accession Number
Gene Names
RETN, FIZZ3, HXCP1, RSTN, UNQ407/PRO1199
Gene ID
56729

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • RETN

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Pektas M., Kurt A., Un I., et al. Effects of 17b-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase. Cytokine. 2015 Apr;72(2):130-4. doi: 10.1016/j.cyto.2014.12.020
    Species: Human
    Sample Type: Conditioned Media

  2. Erturk A., Cure M., Cure E., et al. Clinical potential of resistin as a novel prognostic biomarker for cellulitis. Exp Ther Med. 2015 May;9(5):1875-1880.
    Species: Human
    Sample Type: Serum

  3. Siristatidis C., Askoxylaki M., Varounis C., et al. E-selectin, resistin and reactive oxygen species levels in GnRH -agonist and -antagonist protocols in IVF/ICSI: a prospective cohort study. J Assist Reprod Genet. 2015 Jun;32(6):959-67. doi: 10.1007/s10815-015-0489-z.
    Species: Human
    Sample Type: Serum

  4. Nobili V., Alisi A., Cutrera R., et al. Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver. Thorax. 2015 Aug;70(8):769-81. doi: 10.1136/thoraxjnl-2015-206782
    Species: Human
    Sample Type: Serum

  5. Erturk A, Cure E, Parlak E, Cure MC, Yuce S, Kizilkaya B. Serum resistin levels may be new prognostic factor of crimean-congo hemorrhagic fever. International Journal of Clinical and Experimental Medicine 2014;7(10):3536-3542.
    Species: Human
    Sample Type: Serum

  6. Singh P., et al. Adiponectin-resistin index and its strong association with acute coronary syndrome in South Indian men. Inflammation Research, Sept 2014, epub ahead of print. DOI 10.1007/s00011-014-0771-z
    Species: Human
    Sample Type: Serum

  7. Kerem M, Ferahkose Z, Yilmaz U, Pasaoglu H, Ofluoglu E, et al. Adipokines and ghrelin in gastric cancer cachexia.World J Gastroenterol. 2008;14(23):3633-3641.
    Species: Human
    Sample Type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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