Human SDF-1 alpha ELISA

RayBio® Human SDF-1 alpha (CXCL12 alpha) ELISA Kit for cell culture supernatants and heparin, EDTA, or citrate-treated plasma samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Old price: $402.00
$321.60  
You save: $80.40 (20%)
ELH-SDF1a-1
+

Assay Format

Detection Range:
93.75 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
42 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P48061
Gene ID:
  • 6387
Gene Symbols:
  • CXCL12
  • SDF1
  • SDF1A
  • SDF1B
Protein Name & Synonyms:
Stromal cell-derived factor 1 (SDF-1) (hSDF-1) (C-X-C motif chemokine 12) (Intercrine reduced in hepatomas) (IRH) (hIRH) (Pre-B cell growth-stimulating factor) (PBSF) [Cleaved into: SDF-1-beta(3-72) SDF-1-alpha(3-67)]
Target Species:
  • Human
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Ferrer, Ruben A., et al. "Mesenchymal stromal cells from patients with myelodyplastic syndrome display distinct functional alterations that are modulated by lenalidomide." Haematologica (2013): haematol-2013.
    Species: Human
    Sample type: Conditioned Media (MSCs from myelodyplastic syndrome patients)
  2. Sengenes C, Miranville A, Maumus M, de Barros S, Busse R, Bouloumie A. Chemotaxis and Differentiation of Human Adipose Tissue CD34+/CD31 Progenitor Cells: Role of Stromal Derived Factor-1 Released by Adipose Tissue Capillary Endothelial Cells. Stem Cell. 2007;25:2269-2276.
    Species: Human
    Sample type: Conditioned Media (Human umbilical cord endothelial (HUVEC) , macrophage and adipose tissue capillary endothelial cell-cultured medium)
  3. Shangguan L., Ti X., Krause U., et al. Inhibition of TGF-?/Smad signaling by BAMBI blocks differentiation of human mesenchymal stem cells to carcinoma-associated fibroblasts and abolishes their protumor effects. Stem Cells. 2012 Dec;30(12):2810-9. doi: 10.1002/stem.1251.
    Species: Human
    Sample type: Conditioned Media (Mesenchymal Stem Cells)
  4. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 - 1883
    Species: Human
    Sample type: Serum
  5. Sobrino T., Perez-Mato M., Brea D., et al. Temporal profile of molecular signatures associated with circulating endothelial progenitor cells in human ischemic stroke. J Neurosci Res. 2012 Sep;90(9):1788-93. doi: 10.1002/jnr.23068.
    Species: Human
    Sample type: Serum (Ischemic stroke patients and endothelial progenitor cells.)
  6. Sobrino, Tomás, et al. "Cd34+ progenitor cells likely are involved in the good functional recovery after intracerebral hemorrhage in humans." Journal of neuroscience research 89.7 (2011): 979-985.
    Species: Human
    Sample type: Serum
  7. Vizio, Barbara, Fiorella Biasi, and Giuseppe Poli. "Pancreatic-carcinoma-cell-derived Pro-angiogenic Factors Can Induce Endothelial-cell Differentiation of a Subset of Circulating CD34+ Progenitors." Journal of Translational Medicine. NCBI, 17 Dec. 2013. Web.
    Species: Human
    Sample type: Conditioned Media (cell-free)
  8. Witberg, Guy et al. “Vascular endothelium function among male carriers of BRCA 1&2 germline mutation.” Oncotarget vol. 10,49 5041-5051. 20 Aug. 2019, doi:10.18632/oncotarget.27118
    Species: Human
    Sample type: Plasma
  9. Dudás, József, et al. "Curcumin targets fibroblast–tumor cell interactions in oral squamous cell carcinoma." Experimental cell research 319.6 (2013): 800-809.
    Species: Human
    Sample type: Conditioned Media ( fibroblasts and SCC-25 cells)
  10. Shen C., Lin L., Feng T., et al. Dental Pulp Stem Cells derived Conditioned Medium Promotes Angiogenesis in Hindlimb Ischemia. DOI:10.1007/s13770-014-9053-7Tissue Engineering and Regenerative Medicine February 2015, Volume 12, Issue 1, pp 59-68 .
    Species: Human
    Sample type: Conditioned Media (Human Dental Pulp samples)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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