Human sICAM-1 ELISA

RayBio® Human ICAM-1 (CD54) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$402.00  
ELH-ICAM1-1
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Assay Format

Detection Range:
150 pg/ml - 20 ng/ml
Recommended Dilution (serum & plasma):
5 - 200 fold
Sensitivity:
150 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P05362
Gene ID:
  • 3383
Gene Symbols:
  • ICAM1
Protein Name & Synonyms:
Intercellular adhesion molecule 1 (ICAM-1) (Major group rhinovirus receptor) (CD antigen CD54)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Benagiano M, Munari F, Ciervo A, et al. Chlamydophila pneumoniae phospholipase D (CpPLD) drives Th17 inflammation in human atherosclerosis. Proceedings of the National Academy of Sciences of the United States of America 2012;109(4):1222-1227. doi:10.1073/pnas.1111833109.
    Species: Human
    Sample type: Conditioned Media (Plaque isolated T cells)
  2. Li, Y., Sun, B., Shi, Y., Jiang, J., Du, Z., Chen, R., … Sun, Z. (2020). Subacute exposure of PM2.5 induces airway inflammation through inflammatory cell infiltration and cytokine expression in rats. Chemosphere, 251, 126423. doi:10.1016/j.chemosphere.2020.126423 
    Species: Human
    Sample type: Conditioned media (cell culture media, BEAS-2B )
  3. Chen, Xia, et al. "Lanthanum chloride inhibits LPS mediated expressions of pro-inflammatory cytokines and adhesion molecules in HUVECs: involvement of NF-?B-Jmjd3 signaling." Cellular Physiology and Biochemistry 42.5 (2017): 1713-1724.
    Species: Human
    Sample type: Conditioned Media
  4. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 - 1883
    Species: Human
    Sample type: Serum
  5. Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
    Species: Human
    Sample type: Serum (Lupus Nephritis patients)
  6. Tammali, Ravinder, et al. "Inhibition of aldose reductase prevents angiogenesis in vitro and in vivo." Angiogenesis 14.2 (2011): 209-221.
    Species: Human
    Sample type: Conditioned Media
  7. Nagaraja V, Spino C, Bush E, Tsou PS, Domsic RT, Lafyatis R, Frech T, Gordon JK, Steen VD, Khanna D. A multicenter randomized, double-blind, placebo-controlled pilot study to assess the efficacy and safety of riociguat in systemic sclerosis-associated digital ulcers. Arthritis Res Ther. 2019 Sep 3;21(1):202. doi: 10.1186/s13075-019-1979-7. PMID: 31481106; PMCID: PMC6724329.
    Species: Human
    Sample type: Plasma (patients)
  8. Liu, Zhao-Min, et al. "Effect of soy protein and isoflavones on blood pressure and endothelial cytokines: a 6-month randomized controlled trial among postmenopausal women." Journal of hypertension 31.2 (2013): 384-392.
    Species: Human
    Sample type: Serum (postmenopausal women with pre or early diabetes w)
  9. Bala, Kanchan, Kiran Ambwani, and Nivedita Karmakar Gohil. "Effect of different mitogens and serum concentration on HUVEC morphology and characteristics: Implication on use of higher passage cells." Tissue and Cell 43.4 (2011): 216-222.
    Species: Human
    Sample type: Cell Lysate (HUVEC)
  10. Chao, Pi-Yu, Ya-Ping Huang, and Wen-Bin Hsieh. "Inhibitive effect of purple sweet potato leaf extract and its components on cell adhesion and inflammatory response in human aortic endothelial cells." Cell adhesion & migration 7.2 (2013): 237-245.
    Species: Human
    Sample type: Conditioned Media (effects of pytochemicals and aspirin on the HAECs surface expression)
I recommend the ELISA kits from RayBiotech. Easy to follow with cell culture supernatant.

Florida A&M University
Very sensitive assay system at great price. Highly recommended.

University of South Alabama
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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