Human TGF-beta 1 ELISA

RayBio® Human TGF beta 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Old price: $723.60
$578.88  
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ELH-TGFb1-2
+

Assay Format

Detection Range:
18 pg/ml - 4000 pg/ml
Recommended Dilution (serum & plasma):
20 fold after treatment (see activation steps on page 6)
Sensitivity:
18 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1alpha, TIMP-1, TIMP-2, TNF-alha, TGF-beta 2, TGF-beta 3, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P01137
Gene ID:
  • 7040
Gene Symbols:
  • TGFB
  • TGFB1
Protein Name & Synonyms:
Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)]
Target Species:
  • Human

Target Background

Transforming growth factor β1 (TGFB1) is a cytokine that has an important role in a number of different cellular functions, such as, proliferation, differentiation, chemotaxis, apoptosis and tumor suppression. It plays a crucial role in bone metabolism where is acts as a coupling factor between bone resorption and bone formation. TGFB1 has affinity to TGFB1, 2 and 3 receptors. Downstream TGFB1 signaling activates Smad proteins, mitogen-activated protein kinases and protein kinase B. Mutations in the gene can result in Camurati-Engelmann disease (characterized by increased bone density). Numerous cancer cells are also known to secrete increased levels of TGFB1.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Lippert E., Falk W., Bataille F., et al. Soluble galectin-3 is a strong, colonic epithelial-cell-derived, lamina propria fibroblast-stimulating factor. Gut . 2007 Jan;56(1):43-51. doi: 10.1136/gut.2005.081646. Epub 2006 May 18.
    Species: Human
    Sample type: Conditioned Media (Colonic Epithelial Cells')
  2. Jah A., Tharp K., Ye J., et al. Enhanced survival and engraftment of transplanted stem cells using growth factor sequestering hydrogels. Biomaterials. 2015 Apr;47:1-12. doi: 10.1016/j.biomaterials.2014.12.043.
    Species: Human
    Sample type: Conditioned Media (Hydrogel released TGFb)
  3. Han Y, Xu X, Tang C, Gao P, Chen X, Xiong X, Yang M, Yang S, Zhu X, Yuan S, Liu F, Xiao L, Kanwar YS, Sun L. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biol. 2018 Jun;16:32-46. doi: 10.1016/j.redox.2018.02.013. Epub 2018 Feb 15. Erratum in: Redox Biol. 2019 Jun;24:101216. PMID: 29475133; PMCID: PMC5842313.
    Species: Human/mouse
    Sample type: (HK-2 Cells)
  4. Li, J., Pu, T., Yin, L., Li, Q., Liao, C.-P., & Wu, B. J. (2020). MAOA-mediated reprogramming of stromal fibroblasts promotes prostate tumorigenesis and cancer stemness. Oncogene. doi:10.1038/s41388-020-1217-4 
    Species: Human
    Sample type: Conditioned media (Cell culture media)
  5. Jha A., Mathur A., Svedlund F., et al. Molecular weight and concentration of heparin in hyaluronic acid-based matrices modulates growth factor retention kinetics and stem cell fate. J Control Release. 2015 Jul 10;209:308-16. doi: 10.1016/j.jconrel.2015.04.034.
    Species: Human
    Sample type: Conditioned Media (Incorporation of TGFb into hydrogels.)
  6. Szczepanski, Miroslaw J., et al. "Blast-derived microvesicles in sera from patients with acute myeloid leukemia suppress natural killer cell function via membrane-associated transforming growth factor-?1." Haematologica 96.9 (2011): 1302-1309.
    Species: Human
    Sample type: Serum
  7. Szczepanski, Miroslaw J., et al. "Blast-derived microvesicles in sera of acute myeloid leukemia patients suppresses NK cell function via membrane-associated TGF-?1." Haematologica (2011): haematol-2010.
    Species: Human
    Sample type: Serum (AML and control patients)
  8. Diaz-Gomez L., Concheiro A., Alvarez-Lorenzo C., Garcia-Gonzalez C. Growth factors delivery from hybrid PCL-starch scaffolds processed using supercritical fluid technology. Carbohydr Polym. 2016 May 20;142:282-92. doi: 10.1016/j.carbpol.2016.01.051.
    Species: Human
    Sample type: Conditioned Media (PCL-Starch scaffolds)
  9. Xing W., Xiao Y., Lu., et al. GFI1 downregulation promotes inflammation-linked metastasis of colorectal cancer. Cell Death Differ. 2017 May; 24(5): 929–943.
    Species: Human
    Sample type: Conditioned Media (Various cancer cell lines)
  10. Tang J., Chen Q., Guo J., et al. Minocycline Attenuates Neonatal Germinal-Matrix-Hemorrhage-Induced Neuroinflammation and Brain Edema by Activating Cannabinoid Receptor 2. Mol Neurobiol. 2015 Apr 2. epub ahead of print
    Species: Rat
    Sample type: Tissue Lysate (Brain lysates in matrix Hemorrhage-Induced mice)

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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