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| CODE | Quantibody Size | Price | ||
|---|---|---|---|---|
|
QAH-TH-1-2
|
$756.00 | ||
|
|
QAH-TH-1-1
|
$420.00 | ||
|
|
QAH-TH-1-4
|
$1,344.00 |
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Assay Format
Pathway:
- JAK/STAT Signaling
Number of Targets Detected:
10
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Protein Information
Gene Symbols:
- CSF2
- CXCL8
- IFNG
- IL10
- IL13
- IL2
- IL4
- IL5
- IL6
- TNF
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-10
| IL-13
| IL-2
|
IL-4
| IL-5
| IL-6
| IL-8 (CXCL8)
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Th1/Th2 Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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Schwarz C, Klaus DA, Tudor B, Fleischmann E, Wekerle T, Roth G, et al. (2015)Transection Speed and Impact on Perioperative Inflammatory Response - A Randomized Controlled Trial Comparing Stapler Hepatectomy and CUSA Resection. PLoS ONE 10(10): e0140314. doi:10.1371/journal.pone.0140314
Species: Human
Sample type: Serum -
Chia C., Kumari U., Casey P. Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2. Journal of Molecular Signaling 2014, 9:6 doi:10.1186/1750-2187-9-6
Species: Human
Sample type: Conditioned Media -
Epanchintsev A., Pavithra S., Verma R., Lyakhovich A. IL-6, IL-8, MMP-2, MMP-9 are overexpressed in Fanconi anemia cells through a NF-?B/TNF-? dependent mechanism
Species: Human
Sample type: Conditioned Media -
Veiga P., Schroth R., Guedes R., Freire S., Nogueira-Filho G., Serum cytokine profile among Brazilian children of African descent with periodontal inflammation and sickle cell anaemia. Archives of Oral Biology Volume 58, Issue 5, May 2013, Pages 505-510
Species: Human
Sample type: Serum -
Knotkova, H. et al. Using Transcranial Direct Current Stimulation to Treat Depression in HIV-Infected Persons: The Outcomes of a Feasibility Study. Front Psychiatry. 2012 Jun 18;3:59. doi: 10.3389/fpsyt.2012.00059. eCollection 2012.
Species: Human
Sample type: Serum -
Green V., Michno A., Greenman J., Stafford N. Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients. Results Immunol. 2012; 2: 1-6. Published online 2011 Dec 17. doi: 10.1016/j.rinim.2011.12.001
Species: Human
Sample type: Serum -
Fleming, J. O., et al. "Probiotic helminth administration in relapsingremitting multiple sclerosis: a phase 1 study." Multiple Sclerosis Journal 17.6 (2011): 743-754.
Species: Human
Sample type: Serum -
Gao QT, Cheung JKH, Li J, Jiang ZY, Chu GKY, et al. A Chinese herbal decoction, Danggui Buxue Tang, activates extracellular signal-related kinase in cultured T-lymphocytes. FEBS Lett. 2007;581: 5087-5099.
Species: Human
Sample type: Conditioned Media
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
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