Human Th1/Th2/Th17 Array G1

RayBio® G-Series Human T-Helper Cell 1/2/17 Antibody Array 1 Kit. Detects 34 Human Th1, Th2, and Th17 Cytokines. Suitable for all liquid sample types.

$540.35
AAH-TH17-G1-4
+

Antigen Information

Gene Symbols:
CCL20 | CD40 | CD40LG | CSF2 | CSF3 | IFNG | IFNL2 | IL10 | IL12A | IL12B   View more

Assay Format

Pathway:
  • NFkB Signaling
Number of Targets Detected:
34
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
CD30
(TNFRSF8)
CD40 Ligand
(TNFSF5)
CD40
(TNFRSF5)
GCSF
GITR
(TNFRSF18)
GM-CSF
IFN-gamma
IL-1 beta
(IL-1 F2)
IL-1 R1
IL-1 R2
IL-2
IL-4
IL-5
IL-6
IL-6 R
IL-10
IL-12 p40
IL-12 p70
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-17F
IL-17 RA
IL-21
IL-21 R
IL-22
IL-23
IL-28A
(IFN-lambda 2)
MIP-3 alpha
(CCL20)
gp130
TGF beta 1
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
TNF beta
(TNFSF1B)
TRANCE
(TNFSF11)
  • Gene Symbol: TNFSF11
  • Gene ID: 8600
  • Accession #: O14788

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Th1/Th2/Th17 Antibody Array G1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
This antibody array's result makes a good supplement to our flow cytometry analysis to the human T cell subsets in peripheral blood.
Write A Review
U.S. Non-U.S.

Analysis Tool Bundle Discount

Specify Size
1 x $540.35
+
1 x $116.15 $34.84

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $656.50
Price for all $575.19

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.