Number of Targets Detected:
34
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Solid Support:
Glass Slide
Gene Symbols:
CCL20 | CD40 | CD40LG | CSF2 | CSF3 | IFNG | IFNL2 | IL10 | IL12A | IL12B
| IL13 | IL17A | IL17F | IL17RA | IL1B | IL1R1 | IL1R2 | IL2 | IL21 | IL21R | IL22 | IL23A | IL4 | IL5 | IL6 | IL6R | IL6ST | LTA | TGFB1 | TGFB3 | TNF | TNFRSF18 | TNFRSF8 | TNFSF11  View more  Hide
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Scroll over each target protein for more information |
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| | | | IL-17A- Gene Symbol: IL17A
- Gene ID: 3605
- Accession #: Q16552
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TGF beta 3- Gene Symbol: TGFB3
- Gene ID: 7043
- Accession #: P10600
| | | TRANCE (TNFSF11)- Gene Symbol: TNFSF11
- Gene ID: 8600
- Accession #: O14788
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Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Th1/Th2/Th17 Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
Learn MoreProtocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.