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Home/Search results/Products/Arrays/Antibody Arrays/G-Series: Glass Slide-Based Arrays/Th1/Th2/Th17 Arrays/Human Th1/Th2/Th17 Array G1

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Description
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Antigen Information

Gene Symbols:

CCL20 | CD40 | CD40LG | CSF2 | CSF3 | IFNG | IFNL2 | IL10 | IL12A | IL12B View more

Assay Format

Pathway:

NFkB Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

4 or 8 Sample Kit

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
CD30 (TNFRSF8) Gene Symbol: CD30 Gene ID: 943 Accession #: P28908 ELISA Kit: RayBio Human CD30 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Gene ID: 8784 Accession #: Q9Y5U5 ELISA Kit: RayBio Human GITR ELISA
GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-1 R1 Gene Symbol: IL1R1 Gene ID: 3554 Accession #: P14778 ELISA Kit: RayBio Human IL-1 sRI ELISA	IL-1 R2 Gene Symbol: IL1R2 Gene ID: 7850 Accession #: P27930 ELISA Kit: RayBio Human IL-1 sRII ELISA
IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-6 R Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA
IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552
IL-17F Gene Symbol: IL17F Gene ID: 112744 Accession #: Q96PD4 ELISA Kit: RayBio Human IL-17F ELISA	IL-17 RA Gene Symbol: IL17RA Gene ID: 23765 Accession #: Q96F46 ELISA Kit: RayBio Human IL-17 R ELISA	IL-21 Gene Symbol: IL21 Gene ID: 59067 Accession #: Q9HBE4 ELISA Kit: RayBio Human IL-21 ELISA	IL-21 R Gene Symbol: IL21R Gene ID: 50615 Accession #: Q9HBE5 ELISA Kit: RayBio Human IL-21 R ELISA	IL-22 Gene Symbol: IL22 Gene ID: 50616 Accession #: Q9GZX6 ELISA Kit: RayBio Human IL-22 ELISA
IL-23 Gene Symbol: IL23A Gene ID: 51561 Accession #: Q9NPF7 ELISA Kit: RayBio Human IL-23 ELISA	IL-28A (IFN-lambda 2) Gene Symbol: IFNL2 Gene ID: 282616 Accession #: Q8IZJ0 ELISA Kit: RayBio Human IL-28A ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA	gp130 Gene Symbol: Il6st Gene ID: 3572 Accession #: P40189 ELISA Kit: RayBio Human sgp130 ELISA	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA
TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Gene ID: 8600 Accession #: O14788

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Th1/Th2/Th17 Antibody Array G1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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