Human TIMP-1 ELISA

RayBio® Human TIMP-1 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$402.00  
ELH-TIMP1-1
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Antigen Information

Accession Number:
  • P01033
Gene ID:
  • 7076
Gene Symbols:
  • CLGI
  • TIMP
  • TIMP1
Protein Name & Synonyms:
Metalloproteinase inhibitor 1 (Erythroid-potentiating activity) (EPA) (Fibroblast collagenase inhibitor) (Collagenase inhibitor) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)
Target Species:
  • Human

Assay Format

Detection Range:
40 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
5 - 100 fold
Sensitivity:
40 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1 alpha, TNFbeta, TGFbeta1, TGFbeta2, TGFbeta3, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Moir, L. M., et al. "Doxycycline inhibits matrix metalloproteinase?2 secretion from TSC2?null mouse embryonic fibroblasts and lymphangioleiomyomatosis cells." British journal of pharmacology 164.1 (2011): 83-92.
    Species: Human
    Sample type: Conditioned Media
  2. Garratt L., Sutanto E., Ling K., et al. Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis. Eur Respir J. 2015 Aug;46(2):384-94. doi: 10.1183/09031936.00212114.
    Species: Human
    Sample type: BAL Fluid (CF and control patients)
  3. Raghu H., Nalla A., Gondi C., et al. uPA and uPAR shRNA inhibit angiogenesis via enhanced secretion of SVEGFR1 independent of GM-CSF but dependent on TIMP-1 in endothelial and glioblastoma cells. Mol Oncol. 2012 Feb;6(1):33-47. doi: 10.1016/j.molonc.2011.11.008.
    Species: Human
    Sample type: Conditioned Media (Human primary and xenograft glioma or glioblastoma cell lines)
  4. Hampel U, Klonisch T, Sel S, et al. Insulin-like Factor 3 Promotes Wound Healing at the Ocular Surface. Endocrinology. 2013;154(6):2034-2045.
    Species: Human
    Sample type: Conditioned Media (sebaceous cell line, conjunctival epithelial cell line, corneal epithelial cell line)
  5. Botta GP, Reichert M, Reginato MJ, et al. ERK2-regulated TIMP1 induces hyperproliferation of K-Ras(G12D)-transformed pancreatic ductal cells. Neoplasia. 2013;15(4):359-372.
    Species: Human
    Sample type: Conditioned Media (immortalized primary human pancreatic ductal epithelial cells (PDCs))
  6. Kralisch S, Lossner U, Bluher M, Paschke R, Stumvoll M, Fasshauer M. Tissue inhibitor of metalloproteinase 1 expression and secretion are induced by beta-adrenergic stimulation in 3T3-L1 adipocytes. J Endocrinol. 2006; 189:665-670.
    Species: Human
    Sample type: Conditioned Media (Adipocyte (3T3-L1) cell-conditioned medium)
  7. Khadjavi A., Magnetto C., Panariti A., et al. Chitosan-shelled oxygen-loaded nanodroplets abrogate hypoxia dysregulation of human keratinocyte gelatinases and inhibitors: New insights for chronic wound healing. Toxicol Appl Pharmacol. 2015 Aug 1;286(3):198-206. doi: 10.1016/j.taap.2015.04.015
    Species: Human
    Sample type: Conditioned Media (HaCaT cells keratinocytes)
  8. Basilico N., Magnetto C., D'Alessandro D., et al. Dextran-shelled oxygen-loaded nanodroplets reestablish a normoxia-like pro-angiogenic phenotype and behavior in hypoxic human dermal microvascular endothelium. Toxicol Appl Pharmacol. 2015 Nov 1;288(3):330-8. doi: 10.1016/j.taap.2015.08.005.
    Species: Human
    Sample type: Conditioned Media (Human dermal endothelial cells (HMEC-1))
  9. Ciaffi L., Cavassini M., Genne D., et al. Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study. Eur J Clin Invest. 2015 Jul;45(7):720-30. doi: 10.1111/eci.12464.
    Species: Human
    Sample type: Serum (etravirine treated HIV patients)
  10. Gulino G., Magnetto C., Khadjavi A., et al. Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes. Mediators Inflamm. 2015;2015:964838. doi: 10.1155/2015/964838.
    Species: Human
    Sample type: Conditioned Media (Human Adherent Monocytes)

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Not for diagnostic or therapeutic use.

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