RayBio® Human VCAM-1 (CD106) ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • P19320
Gene ID:
  • 7412
Gene Symbols:
  • L1CAM
  • VCAM1
Protein Name & Synonyms:
Vascular cell adhesion protein 1 (V-CAM 1) (VCAM-1) (INCAM-100) (CD antigen CD106)
Target Species:
  • Human

Assay Format

Detection Range:
0.3 ng/ml - 60 ng/ml
Recommended Dilution (serum & plasma):
10 - 50 fold
300 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4, DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, MCSF R, MMP-1, 2, 3, 7, 8, 9, 10,12 and 13, PDGF-AB, SDF-1b, Tie-1, Tie- 2, TIMP-3.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Fadel F., Elshamaa M., Nabhan M., Essam R., Kantoush N., et al. Soluble adhesion molecules as markers of nativearteriovenous fistula thrombosis in children on uremia. Blood Coagulation and Firbrinolysis. Published online DOI:10.1097/MBC.0000000000000125 [Epub ahead of Print]
    Species: Human
    Sample type: Serum
  2. Wadood S., Shawk R., Baher H. Intercellular Adhesion Molecule-1, Vascular Adhesion Molecule-1, Interlukin1-?, and C - reactive protein Levels in Iraqi Patients with Type 2 Diabetes Mellitus. IOSR J of Dental and Medical SciencesVolume 13, Issue 4 Ver. IV. (Apr. 2014), PP55-62
    Species: Human
    Sample type: Serum
  3. Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes
    Species: Human
    Sample type: Vitreous Fluid
  4. Schlatzer DM, Sugalski JM, Chen Y, et al. Plasma proteome analysis reveals overlapping, yet distinct mechanisms of immune activation in chronic HCV and HIV infections. J Acquir Immune Defic Syndr. 2013 Mar 15; [Epub ahead of print] doi:10.1097/QAI.0b013e3182909847.
    Species: Human
    Sample type: Plasma
  5. Ro, Han, et al. "Roles of islet Toll-like receptors in pig to mouse islet xenotransplantation."?Cell transplantation?22.9 (2013): 1709-1722.
    Species: Porcine
    Sample type: Conditioned Media
  6. Liu, Zhao-Min, et al. "Effect of soy protein and isoflavones on blood pressure and endothelial cytokines: a 6-month randomized controlled trial among postmenopausal women."?Journal of hypertension?31.2 (2013): 384-392.
    Species: Human
    Sample type: Serum
  7. Chao, Pi-Yu, Ya-Ping Huang, and Wen-Bin Hsieh. "Inhibitive effect of purple sweet potato leaf extract and its components on cell adhesion and inflammatory response in human aortic endothelial cells."?Cell adhesion & migration?7.2 (2013): 237-245.
    Species: Human
    Sample type: Conditioned Media
  8. Lin, Kuan-Hung, et al. "Chlorophyll-related compounds inhibit cell adhesion and inflammation in human aortic cells."?Journal of medicinal food?16.10 (2013): 886-898.
    Species: Human
    Sample type: Cell Lysate
  9. Chao, Pi-Yu, et al. "Effects of black soybean on atherogenic prevention in hypercholesterolemic rabbits and on adhesion molecular expression in cultured HAECs."?Food and Nutrition Sciences?4.08 (2013): 9.
    Species: Human
    Sample type: Cell Lysate
  10. Asato, Ryo, et al. "Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes."?PLoS One?8.1 (2013): e54191.
    Species: Human
    Sample type: vitreous fluid

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