Human VEGF-A ELISA

RayBio® Human VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$420.00  
ELH-VEGF-1
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Assay Format

Detection Range:
10 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 - 5 fold
Sensitivity:
10 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P15692
Gene ID:
  • 7422
Gene Symbols:
  • VEGF
  • VEGFA
Protein Name & Synonyms:
Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Target Species:
  • Human

Target Background

VEGF is a member of the PDGF/VEGF growth factor family that is often found as a disulfide linked homodimer. It is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in VEGF gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Human VEGF ELISA Kit Standard Curves

Human VEGF ELISA Dilution Series Diagram

Spiking & Recovery Results

VEGF ELISA Spiking and Recovery Results

Linearity Results

VEGF ELISA Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Gruene, Martin, et al. "Laser printing of three-dimensional multicellular arrays for studies of cell–cell and cell–environment interactions." Tissue Engineering Part C: Methods 17.10 (2011): 973-982.
    Species: Human
    Sample type: Conditioned Media
  2. Ruan, Qing, et al. "?B-crystallin, an effector of unfolded protein response, confers anti-VEGF resistance to breast cancer via maintenance of intracrine VEGF in endothelial cells." Molecular Cancer Research 9.12 (2011): 1632-1643.
    Species: Human
    Sample type: Conditioned Media (HMECs co-cultured with breast tumor cells)
  3. Moneib H., Salem S., Aly D., et al. Assessment of serum vascular endothelial growth factor and nail fold capillaroscopy changes in systemic lupus erythematosus with and without cutaneous manifestations. J Dermatol. 2012 Jan;39(1):52-7. doi: 10.1111/j.1346-8138.2011.01322.x.
    Species: Human
    Sample type: Serum (SLE biomarer study (lupus))
  4. Sakellariou G., et al. Circulating periostin levels in patients with AS: association with clinical and radiographic variables, inflammatory markers and molecules involved in bone formation. Rheumatology (2014) doi:10.1093/rheumatology/keu425
    Species: Human
    Sample type: Serum (Anti-tnf treatment atherosclerosis patients)
  5. Chen D., et al. Increased expression of Id1 and Id3 promotes tumorigenicity by enhancing angiogenesis and suppressing apoptosis in small cell lung cancer. Genes Cancer. May 2014; 5(5-6): 212-225
    Species: Human
    Sample type: Conditioned Media
  6. Chen C-J, Zhao Z-X, Wang J-C, et al. A comparative study of three ternary complexes prepared in different mixing orders of siRNA/redox-responsive hyperbranched poly (amido amine)/hyaluronic acid. International Journal of Nanomedicine 2012;7:3837-3849. doi:10.2147/IJN.S32676.
    Species: Human
    Sample type: Conditioned Media (Breat cancer cells, MCF-7 and MDA-MB-231)
  7. Song H, Wang G, He B, et al. Cationic lipid-coated PEI/DNA polyplexes with improved efficiency and reduced cytotoxicity for gene delivery into mesenchymal stem cells. International Journal of Nanomedicine 2012;7:4637-4648. doi:10.2147/IJN.S33923.
    Species: Human
    Sample type: Conditioned Media (Bone Marow Mesenchymal Stem cells)
  8. Guex AG., et al. Covalent immobilisation of VEGF on plasma-coated electrospunscaffolds for tissue engineering applications. Colloids and Surfaces B: Biointerfaces 123 (2014) 724-733. http://dx.doi.org/10.1016/j.colsurfb.2014.10.016
    Species: Human
    Sample type: Conditioned Media (Substrate immobilized plasma-coated scaffolds)
  9. Mohandas A., Anisha BS., Chennazhi KP., Jayakumar R. Chitosan-hyaluronic acid/VEGF loaded fibrin nanoparticles composite sponges for enhancing angiogenesis in wounds. Colloids Surf B Biointerfaces. 2015 Mar 1;127:105-13. doi: 10.1016/j.colsurfb.2015.01.024
    Species: Human
    Sample type: Conditioned Media (VEGF loaded chitosan-hyaluronic acid laded sponges)
  10. Rajoria S, Suriano R, George AL, et al. Molecular target based combinational therapeutic approaches in thyroid cancer. Journal of Translational Medicine. 2012;10:81. doi:10.1186/1479-5876-10-81.
    Species: Human
    Sample type: Conditioned Media (Thyroid Cancer Cells)
It is a very user-friendly product for people who work with this type of sample for the first time, highly recommended.

University of Puerto Rico
This kit is simple to use and ideal for those who are new to evaluating factors on culture media. All directions are simple to follow, and the reagents are high quality.

University of Puerto Rico at Mayaguez
Very good sensitivity

BCM
Had great success using these kits. Really clear results and effective. These kits are easy to use and come with all the components needed which is a plus! Look forward to using them again.

Advirna
I've used this product several times and am very happy with its high sensitivity, ease of use, and the technical support.

State University of New York
I used cell culture supernatant and it was really easy to perform

CPMC
Use it to test in vitro releasing of VEGF-A from tissue engineering scaffold. Easy to use and work very well.

North Carolina State University
I used this kit for first time and works really good

CPMC
Very sensitive kit, instructions are easy to follow and have yielded great results!

University of Illinois
Writing another review because we have had nothing but success with this kit! Looking to publish the results soon!

University of Florida
Easy to use!

University of San Diego
The ELISA instructions are concise and easy to follow. The kit also has all reagents you need. It has great reproducibility and reliability.

University of Florida
This product has been reliable with a descriptive protocol booklet that is easy to follow.

Immutrix Therapeutics
Very simple, concise instructions. This ELISA kit has a wide concentration detection range. It performed as intended and will continue using their ELISA kits. I fully recommend RayBiotech to anyone in lab.

Stanford University
We ordered 2 boxes of this kit (along side with 2 of Periostin and 2 of IL-4 ELISA kits). The kits came in strong, sturdy boxes. The instructions are clear and easy to follow. The only thing is we wish that the sensitivity was lower. The current minimum detectable dose of Human VEGFA is 10 pg/ml. We had to dilute the standards and remove one of the them from the standard curve to be able to detect the protein levels. But we used these kits on urine samples and it seemed like VEGFA was very low in urine anyways. Just an idea for future improvement.

New York Medical College
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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