Human VEGF R2 ELISA

RayBio® Human VEGFR2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-VEGFR2-1
+

Antigen Information

Accession Number:
  • P35968
Gene ID:
  • 3791
Gene Symbols:
  • FLK1
  • KDR
  • VEGFR2
Protein Name & Synonyms:
Vascular endothelial growth factor receptor 2 (VEGFR-2) (EC 2.7.10.1) (Fetal liver kinase 1) (FLK-1) (Kinase insert domain receptor) (KDR) (Protein-tyrosine kinase receptor flk-1) (CD antigen CD309)
Target Species:
  • Human

Assay Format

Detection Range:
70 pg/ml - 25000 pg/ml
Recommended Dilution (serum & plasma):
10 - 100 fold
Sensitivity:
70 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiopoietin-1, Angiostatin , BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, ESelectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Choudhuri S., Chowdhury I., Das S., et al. Role of NF-?B activation and VEGF gene polymorphisms in VEGF up regulation in non-proliferative and proliferative diabetic retinopathy. Mol Cell Biochem. 2015 Jul;405(1-2):265-79. doi: 10.1007/s11010-015-2417-z
    Species: Human
    Sample type: Serum
  2. Kalita J., Chauhan P., Mani V., et al. VEGF and its receptors in dengue virus infection. J Med Virol. 2015 Sep;87(9):1449-55. doi: 10.1002/jmv.24205.
    Species: Human
    Sample type: Serum
  3. Goswami B., Goyal M., Beri S., et al. Role of Serum Levels of Vascular Endothelial Growth Factor and Its Receptor in Retinopathy of Prematurity. Iran J Pediatr. 2015 Aug; 25(4): e2373. doi: 10.5812/ijp.237
    Species: Human
    Sample type: Serum
  4. Zheng YB., et al. Genetic variants in the KDR gene is associated with the prognosis of transarterial chemoembolization treated hepatocellular carcinoma. Tumor Biol. 2014. Epub Ahead of print. DOI 10.1007/s13277-014-2478-8
    Species: Human
    Sample type: Serum
  5. Zheng YB., et al. The relationship of kinase insert domain receptor gene polymorphisms and clinical outcome in advanced hepatocellular carcinoma patients treated with sorafenib. Med Oncol (2014) 31:209. DOI 10.1007/s12032-014-0209-z
    Species: Human
    Sample type: Serum
  6. Mondal LK, Choudhury S, Paine SK, et al. Anaerobic Glycolysis is the Centre of Different Biochemical Disarrays Associated with the Onset of Diabetic Retinopathy. J Evol Med Dent Sci. 2013 Apr 8;(924).
    Species: Human
    Sample type: Serum
  7. Navid F, Baker SD, McCarville B, et al. Phase I and Clinical Pharmacology Study of Bevacizumab, Sorafenib, and Low-Dose Cyclophosphamide in Children and Young Adults with Refractory/Recurrent Solid Tumors. Clin Cancer Res. 2013;19(1):236-246.
    Species: Human
    Sample type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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