Human VEGF-A ELISA (For Lysates)

RayBio® Human VEGF-A ELISA Kit for cell and tissue lysate samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELH-VEGF-CL-1
+

Antigen Information

Accession Number:
  • P15692
Gene ID:
  • 7422
Gene Symbols:
  • VEGF
  • VEGFA
Target Species:
  • Human

Assay Format

Sensitivity:
10 pg/ml

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Specificity:
The antibody pair provided in this kit recognizes human VEGF-A.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Lysates
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
  • Cell Lysis Buffer
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Dong D., Gao W., Liu Y., Qi XR. Therapeutic potential of targeted multifunctional nanocomplex co-delivery of siRNA and low-dose doxorubicin in breast cancer. Cancer Lett. 2015 Apr 10;359(2):178-86. doi: 10.1016/j.canlet.2015.01.011
    Species: Human
    Sample type: Tissue Lysate
  2. Biswal MR., Prentice HM., Dorey CK., Blanks JC. A hypoxia-responsive glial cell-specific gene therapy vector for targeting retinal neovascularization. Invest Ophthalmol Vis Sci. 2014 Nov 6;55(12):8044-53. doi: 10.1167/iovs.14-13932
    Species: Human
    Sample type: Tissue Lysate
  3. Diaz-Gomez L., et al. Random and Aligned PLLA : PRGF Electrospun Scaffolds for Regenerative Medicine. Journal of Applied Polymer Science August 2014, epub ahead of print. DOI: 10.1002/app.41372
    Species: Human
    Sample type: Cell Lysate
  4. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample type: Tissue Lysate
  5. Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
    Species: Human
    Sample type: Tissue Lysate
  6. Fermoselle C., Rabinovich R., Ausin P., et al. Does oxidative stress modulate limb muscle atrophy in severe COPD patients? Eur Respir J. 2012 Oct;40(4):851-62.
    Species: Human
    Sample type: Tissue Lysate
  7. Enestvedt C., Hosack L., Hoppo T., et al. Recombinant vascular endothelial growth factor165 gene therapy improves anastomotic healing in an animal model of ischemic esophagogastrostomy. Dis Esophagus. 2012 Jul;25(5):456-64. doi: 10.1111/j.1442-2050.2011.01247.x
    Species: Human
    Sample type: Tissue Lysate
  8. Nomura S., Kami K., Kawano F., et al. Effects of hindlimb unloading on neurogenesis in the hippocampus of newly weaned rats. Neurosci Lett. 2012 Feb 16;509(2):76-81. doi: 10.1016/j.neulet.2011.12.022.
    Species: Rat
    Sample type: Tissue Lysate
  9. Reckhenrich, Ann K., et al. "Bioactivation of dermal scaffolds with a non-viral copolymer-protected gene vector." Biomaterials 32.7 (2011): 1996-2003.
    Species: Human
    Sample type: Tissue lysate
  10. Nassar, Zeyad D., et al. "Antiangiogenic properties of Koetjapic acid, a natural triterpene isolated from Sandoricum koetjaoe Merr." Cancer cell international 11.1 (2011): 12.
    Species: Human
    Sample type: Cell Lysate
I'm really impressed by all Raybiotech ELISA kits. This one is not an exception: it works well with tumor tissue lysates, and allows to analyze tumor cell-derived VEGF-A in xenograft mouse models.

Roswell Park Comprehensive Cancer Center
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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