Human VEGF-A ELISA

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$361.80
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ELH-VEGF-1

RayBio® Human VEGF-A ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Antigen Information

Accession Number:
  • P15692
Gene ID:
  • 7422
Gene Symbol:
  • VEGF
  • VEGFA
Protein Name & Synonyms:
Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Target Species:
  • Human

Assay Format

Detection Range:
10 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 - 5 fold
Sensitivity:
10 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Etulain J., Mena H., Meiss R., et al. An optimised protocol for plateletrich plasma preparation to improve its angiogenic and regenerative properties. Scientific Reports (2018) 8:1513 | DOI:10.1038/s41598-018-19419-6
    Species: Human
    Sample type: Conditioned Media
  2. Yildirim N., Dikmen Y., Terek M., et al. Do preoperative serum vascular endothelial growth factor and migration-inhibitory factor predict the nature of the adnexal masses? A prospective-controlled trial. J Obstet Gynaecol. 2016 Jan 13:1-5. Epub ahead of print
    Species: Human
    Sample type: Serum
  3. Wang J., Su X., Yang L., et al. The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery. Int J Cancer. 2016 Jun 1;138(11):2688-99. doi: 10.1002/ijc.29998.
    Species: Human
    Sample type: Serum
  4. Diaz-Gomez L., Concheiro A., Alvarez-Lorenzo C., Garcia-Gonzalez C. Growth factors delivery from hybrid PCL-starch scaffolds processed using supercritical fluid technology. Carbohydr Polym. 2016 May 20;142:282-92. doi: 10.1016/j.carbpol.2016.01.051.
    Species: Human
    Sample type: Conditioned Media
  5. Gamal A., Abdel-Ghaffer K., Iacono V.Gingival crevicular fluid vascular endothelial cell growth factor and platelet-derived growth factor-BB release profile following the use of perforated barrier membranes during treatment of intrabony defects: a randomized clinical trial.J Periodontal Res. 2016 Jun;51(3):407-16. doi: 10.1111/jre.12321
    Species: Human
    Sample type: Gingival Crevicular Fluid
  6. Zhang Z., Zhang H., Peng T., et al. Melittin suppresses cathepsin S-induced invasion and angiogenesis via blocking of the VEGF-A/VEGFR-2/MEK1/ERK1/2 pathway in human hepatocellular carcinoma. Oncol Lett. 2016 Jan; 11(1): 610-618.
    Species: Human
    Sample type: Conditioned Media
  7. Kohh B., Nadarajan K., Shim S., et al. Pretreatment of BMSCs with TZD solution decreases the proliferation rate of MCF?7 cells by reducing FGF4 protein expression. Molecular Medicine Reports 13: 3406-3414, 2016
    Species: Human
    Sample type: Conditioned Media
  8. Wood, Lauren W., et al. "Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome." Scientific reports 6 (2016).
    Species: Human
    Sample type: Conditioned Media
  9. Dong D., Gao W., Liu Y., Qi XR. Therapeutic potential of targeted multifunctional nanocomplex co-delivery of siRNA and low-dose doxorubicin in breast cancer. Cancer Lett. 2015 Apr 10;359(2):178-86. doi: 10.1016/j.canlet.2015.01.011
    Species: Human
    Sample type: Tissue Lysate
  10. Gao Q., Zheng J., Yao X., Peng B. Adiponectin inhibits VEGF-A in prostate cancer cells. Tumour Biol. 2015 Jan, epub ahead of print
    Species: Human
    Sample type: Conditioned Media
Very simple, concise instructions. This ELISA kit has a wide concentration detection range. It performed as intended and will continue using their ELISA kits. I fully recommend RayBiotech to anyone in lab.
We ordered 2 boxes of this kit (along side with 2 of Periostin and 2 of IL-4 ELISA kits). The kits came in strong, sturdy boxes. The instructions are clear and easy to follow. The only thing is we wish that the sensitivity was lower. The current minimum detectable dose of Human VEGFA is 10 pg/ml. We had to dilute the standards and remove one of the them from the standard curve to be able to detect the protein levels. But we used these kits on urine samples and it seemed like VEGFA was very low in urine anyways. Just an idea for future improvement.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.