Human Visfatin EIA

RayBio® Human Visfatin EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.

$380.00  
EIA-VIS-1
+

Assay Format

Detection Range:
0.1-1,000 ng/ml
Recommended Dilution (serum & plasma):
Human: 4X
Sensitivity:
0.778 ng/ml or 6.82 pM
Specificity:
Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, NPY and APC.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Serum
Design Principle:
  • Competition-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P43490
Gene ID:
  • 10135
Gene Symbols:
  • NAMPT
  • PBEF
  • PBEF1
Protein Name & Synonyms:
Nicotinamide phosphoribosyltransferase (NAmPRTase) (Nampt) (EC 2.4.2.12) (Pre-B-cell colony-enhancing factor 1) (Pre-B cell-enhancing factor) (Visfatin)

Introduction

Obesity, which is characterized by excessive accumulation of adipose tissue in the body, has become one of the greatest public health challenges. Obesity is not only associated with health problems linked to increased weight-dependent pressure overload on lung, joints and bones, but also a important risk factor for life-threatening diseases such as cardiovascular diseases, type 2 diabetes and certain cancers.

Visfatin, an adipokine that is highly enriched in the visceral fat, has increased expression level in plasma during the development of obesity, implying its important role in obesity.

Visfatin (also known as PBEF and Nampt), a 52 kDa cytoplasmic protein composed of 473-amino acids, is expressed primarily in bone marrow, muscle and liver tissue but can also be detected in placenta, lung, kidney and heart tissue.

In addition to its role in obesity, visfatin also has other biological functions. Visfatin is a nicotinamide phosphoribosyltransferase (Nampt) enzyme that catalyzes first step in the biosynthesis of NAD from nicotinamide. Visfatin has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  • Prepare all reagents, samples and standards as instructed.
  • Add 100 µl detection antibody to each well.
  • Incubate 1.5 h at RT or O/N at 4°C.
  • Add 100 µl standard or sample to each well.
  • Incubate 2.5 h at RT.
  • Add 100 µl prepared streptavidin solution.
  • Incubate 45 min at RT.
  • Add 100 µl TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl Stop Solution to each well.
  • Read plate at 450 nm immediately.

Storage/Stability

Standard, Biotinylated Visfatin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
  1. Karakan S., Sezer S., Ozdemir A., Haberal M. The relationship of visfatin levels with insulin resistance and left ventricular hypertrophy in peritoneal dialysis patients. Ren Fail. 2012;34(6):732-7. doi: 10.3109/0886022X.2012.676493.
    Species: Human
    Sample type: Serum (Non-diabetic Peritoneal Dialysis patients)
  2. Rodrigues K., Pietrani N., Bosco A., et al. Visfatin levels are decreased in advanced stages of diabetic nephropathy. Ren Fail. 2015;37(9):1529-30. doi: 10.3109/0886022X.2015.1074520.
    Species: Human
    Sample type: Serum (Diabetic nephropathy patients)
  3. Keshk W., Noeman S. Impact of Chicory-Supplemented Diet on HMG-CoA Reductase, Acetyl-CoA Carboxylase, Visfatin and Anti-Oxidant Status in Triton WR-1339-Induced Hyperlipidemia. Journal of Food Biochemistry Vol 39 Iss 2, April 2015, 164-172. DOI: 10.1111/jfbc.12115
    Species: Rat
    Sample type: Serum (Chicory diet)
  4. Guzel S., et al. Visfatin, Leptin, and TNF-?: Interrelated Adipokines in Insulin-Resistant Clinical and Subclinical Hypothyroidism. Endocrine Research. Aug2013, Vol. 38 Issue 3, p184-194. DOI:10.3109/07435800.2012.760588
    Species: Human
    Sample type: Serum
  5. Elgarhy L. Abdelnabi N., Abdullatif A., et al. Circulating endothelial cells and serum visfatin are indicators of cardiovascular disease risk in psoriasis patients. Dermatologica Sinica, Volume 34, Issue 1, March 2016, Pages 20-25, http://dx.doi.org/10.1016/j.dsi.2015.07.004
    Species: Human
    Sample type: Serum (Cardiovascular disease patients with psoriasis)
  6. Mabrouk R., et al. Serum Visfatin, Resistin and IL-18 in A Group of Egyptian Obese Diabetic and Non Diabetic Individuals. THE EGYPTIAN JOURNAL OF IMMUNOLOGY Vol. 20 (1), 2013
    Species: Human
    Sample type: Serum
  7. El-Shafey E., El-Naggar G., Al-Bedewy M., El-Sorogy H. Is There A Relationship Between Visfatin Level and Type 2 Diabetes Mellitus In Obese And Non Obese Patients? J Diabetes Metab 2012, S:11:001http://dx.doi.org/10.4172/2155-6156.S11-001
    Species: Human
    Sample type: Serum (Obese and no obese Type 2 Diabetes patients)
  8. Shaker O., Abdelhafez-M., Fayez S., and Alsherif Y. Phosphatidylinositol 3-Kinase and Insulin Receptor Substrate-1 Gene Polymorphism / Adipocytokines Interaction in Pathogenesis of Insulin Resistance among Egyptian Patients: A Pilot Study. International Journal of Research Studies in Biosciences (IJRSB) Volume 3, Issue 9, September 2015, PP 81-93 ISSN 2349-0357 (Print) & ISSN 2349-0365 (Online)
    Species: Human
    Sample type: Serum (Patients for insulin resistence)
  9. Pisani D., Dumortier O., Beranger G., et al. Visfatin expression analysis in association with recruitment and activation of human and rodent brown and brite adipocytes. Adipocyte. 2016 Apr-Jun; 5(2): 186-195. doi: 10.1080/21623945.2015.1122854
    Species: Human
    Sample type: Conditioned Media (human adipocyte derived stem cells)
  10. Dem?r, B. Cetinkaya, et al. "Maternal adiponectin and visfatin concentrations in normal and complicated pregnancies." Clinical and experimental obstetrics & gynecology 40.2 (2013).
    Species: Human
    Sample type: Serum (pregnancy)
This is a very easy to use kit with extremely good instructions on how to prepare everything that you need for the assay. I would highly recommend any of the ELISA kits from this company.

The University of Michigan
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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