IL-2 Epitope Mapping Peptide Array

The IL-2 Epitope Mapping Peptide Array is a semi-quantitative array displaying 122 synthesized peptides representing the full-length IL-2. It is suitable for antibody epitope mapping and detection of IgG, IgA, IgM, IgE, etc. in serum, plasma, and many other liquid sample types.

For a service quote, please fill out the Epitope Mapping Array Quote Request Form.

[Price not listed]  
PAH-IL2-G1

Assay Format

Species Detected:
  • Human
Compatible Sample Types:
  • Ascites
  • DNA Aptamers
  • Hybridoma cell culture medium
  • Other fluids
  • Plasma
  • Purified antibodies
  • Purified protein
  • Serum
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide
Antibody Type:
Human IgG, mouse IgG, other isotypes (IgA, IgM, IgE, etc.) derived from most animals, upon request.

Product Specifications

Size:
1, 2, or 4 Slide Kit

Protein Information

Accession Number:
  • P60568
Gene Symbols:
  • IL2
Protein Name & Synonyms:
Interleukin-2 (IL-2) (T-cell growth factor) (TCGF) (Aldesleukin)
Target Species:
Human
Synthesized Protein Sequence:
133 amino acids (Ala21-Thr153, without N-terminal signal peptide)
APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT

Peptide Information

Number of Peptides:
122
Peptide Length:
12-mer
Peptide Offset:
1-mer
Peptide Purity:
HPLC grade, >95% purity
Array Printing Replicate:
Triplicate

Peptide Sequence

1. APTSSSTKKTQL 2. PTSSSTKKTQLQ 3. TSSSTKKTQLQL 4. SSSTKKTQLQLE
5. SSTKKTQLQLEH 6. STKKTQLQLEHL 7. TKKTQLQLEHLL 8. KKTQLQLEHLLL
9. KTQLQLEHLLLD 10. TQLQLEHLLLDL 11. QLQLEHLLLDLQ 12. LQLEHLLLDLQM
13. QLEHLLLDLQMI 14. LEHLLLDLQMIL 15. EHLLLDLQMILN 16. HLLLDLQMILNG
17. LLLDLQMILNGI 18. LLDLQMILNGIN 19. LDLQMILNGINN 20. DLQMILNGINNY
21. LQMILNGINNYK 22. QMILNGINNYKN 23. MILNGINNYKNP 24. ILNGINNYKNPK
25. LNGINNYKNPKL 26. NGINNYKNPKLT 27. GINNYKNPKLTR 28. INNYKNPKLTRM
29. NNYKNPKLTRML 30. NYKNPKLTRMLT 31. YKNPKLTRMLTF 32. KNPKLTRMLTFK
33. NPKLTRMLTFKF 34. PKLTRMLTFKFY 35. KLTRMLTFKFYM 36. LTRMLTFKFYMP
37. TRMLTFKFYMPK 38. RMLTFKFYMPKK 39. MLTFKFYMPKKA 40. LTFKFYMPKKAT
41. TFKFYMPKKATE 42. FKFYMPKKATEL 43. KFYMPKKATELK 44. FYMPKKATELKH
45. YMPKKATELKHL 46. MPKKATELKHLQ 47. PKKATELKHLQC 48. KKATELKHLQCL
49. KATELKHLQCLE 50. ATELKHLQCLEE 51. TELKHLQCLEEE 52. ELKHLQCLEEEL
53. LKHLQCLEEELK 54. KHLQCLEEELKP 55. HLQCLEEELKPL 56. LQCLEEELKPLE
57. QCLEEELKPLEE 58. CLEEELKPLEEV 59. LEEELKPLEEVL 60. EEELKPLEEVLN
61. EELKPLEEVLNL 62. ELKPLEEVLNLA 63. LKPLEEVLNLAQ 64. KPLEEVLNLAQS
65. PLEEVLNLAQSK 66. LEEVLNLAQSKN 67. EEVLNLAQSKNF 68. EVLNLAQSKNFH
69. VLNLAQSKNFHL 70. LNLAQSKNFHLR 71. NLAQSKNFHLRP 72. LAQSKNFHLRPR
73. AQSKNFHLRPRD 74. QSKNFHLRPRDL 75. SKNFHLRPRDLI 76. KNFHLRPRDLIS
77. NFHLRPRDLISN 78. FHLRPRDLISNI 79. HLRPRDLISNIN 80. LRPRDLISNINV
81. RPRDLISNINVI 82. PRDLISNINVIV 83. RDLISNINVIVL 84. DLISNINVIVLE
85. LISNINVIVLEL 86. ISNINVIVLELK 87. SNINVIVLELKG 88. NINVIVLELKGS
89. INVIVLELKGSE 90. NVIVLELKGSET 91. VIVLELKGSETT 92. IVLELKGSETTF
93. VLELKGSETTFM 94. LELKGSETTFMC 95. ELKGSETTFMCE 96. LKGSETTFMCEY
97. KGSETTFMCEYA 98. GSETTFMCEYAD 99. SETTFMCEYADE 100. ETTFMCEYADET
101. TTFMCEYADETA 102. TFMCEYADETAT 103. FMCEYADETATI 104. MCEYADETATIV
105. CEYADETATIVE 106. EYADETATIVEF 107. YADETATIVEFL 108. ADETATIVEFLN
109. DETATIVEFLNR 110. ETATIVEFLNRW 111. TATIVEFLNRWI 112. ATIVEFLNRWIT
113. TIVEFLNRWITF 114. IVEFLNRWITFC 115. VEFLNRWITFCQ 116. EFLNRWITFCQS
117. FLNRWITFCQSI 118. LNRWITFCQSII 119. NRWITFCQSIIS 120. RWITFCQSIIST
121. WITFCQSIISTL 122. ITFCQSIISTLT    

Application Notes

  • Identify high antigenicity epitopes for antibody production & vaccine development
  • Locate (auto)antigen epitopes of (auto)antibodies
  • Define the best antibody clone against the protein-of-interest
  • Perform epitope mutant analysis
  • Map B cell epitopes

Service Features

  • Small serum sample volume required (2 µl)
  • High density (simultaneously analyze hundreds of overlapping peptides)
  • High sensitivity
  • Large dynamic range
  • Suitable for high-throughput assays
  • High efficiency and accuracy
  • Affordable, quick and simple to use

Kit Components

  • RayBio® human IL-2 protein peptide array
  • Blocking Buffer
  • Wash Buffer I
  • Wash Buffer II
  • Biotin-conjugated secondary antibody
  • Cy3 equivalent dye conjugated streptavidin
  • Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)

Other Materials Required

  • Distilled water
  • Small plastic boxes or containers
  • Orbital shaker or oscillating rocker
  • Pipettors, pipette tips and other common lab consumables
  • Aluminum foil
  • Laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Storage/Stability

Upon receipt, all kit components should be stored at -20 °C to -80 °C until ready to use. Stored properly, the kit will retain complete activity for up to 6 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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