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Immunome Protein Arrays

Introduction

The RayBio® Immunome Protein Arrays are multiplex protein immunoassays for numerous research applications. Correctly folded, functional proteins are immobilized onto a solid glass slide surface. The slides can then be probed with serum or small molecules and used in protein-antibody studies, small-molecule inhibition of kinase studies, protein-protein studies, methylation studies or DNA binding studies. Our main immunome array product offering contains 1,631 proteins. Smaller custom arrays are also available.

Custom Immunome ArrayView All Protein Array SystemsImmunome Protein Array 1631

Immunome Protein Array Features

Correctly Folded, Functional Proteins

Each 75mm x 25mm glass slide is spotted with 4 identical protein arrays (also called  “subarrays”), each with 1,631 correctly folded and functional spotted proteins! Each protein is attached to the streptavidin-coated slide exclusively via a biotinylated affinity tag, specifically the biotin carboxyl carrier protein (BCCP) domain of the E. coli acetyl CoA carboxylase. This affinity tag was chosen because the BCCP domain must be folded into its native 3-dimensional structure in order to become biotinylated. If the expressed protein mis-folds the BCCP tag will also mis-fold, preventing biotinylation. If the protein folds correctly then folding of the BCCP tag should be unhindered and biotinylation will result. This property allows only correctly folded proteins to be bound to the slide.

Immunome Imbolization Mechanism

Features

  • Less sample, more data: only 22.5 µl of  original sample is needed
  • Less than 8-hour processing time
  • No interference between immobilized proteins (unlike bead-based multiplex assays)
  • No dedicated equipment required

Contents of kit

  • Glass Chip with Protein Arrays
  • Cy3-conjugated Anti-human IgG
  • Pooled normal serum
  • Pap jars
  • Quadriperm dish
  • Staining dish with lid
  • Slide rack
  • User manual
*Instructions for preparing additional reagents included in manual

Research Applications

  • Screening protein-antibody interactions
  • Performing small-molecule-kinase inhibition experiments
  • Screening protein-protein interactions
  • Performing DNA-binding experiments
  • Performing methylation assays

How It Works

A panel of correctly folded, functional proteins is printed in multiple, identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific auto-antibodies are then washed off, and the arrays are incubated with Cy3 labelled anti-human IgG detection antibodies. Signals are then visualized using a fluorescence laser scanner. For a list of laser scanner specifications, click here.

Protein-Antibody Interactions - How it works

A panel of correctly folded, functional proteins is printed in multiple, identical arrays on a standard slide. After a blocking step, controls and inhibitors are incubated with the arrays. Cosubstrates are then added to the array to induce autophosphorylation of kinases. Labeled anti-phospho antibodies are added, and bind relative to the phosphorylated kinases. Relative signals are then visualized using a fluorescence laser scanner. For a list of laser scanner specifications, click here.

Small Molecule - Kinase inhibition assay - How it works

A panel of correctly folded, functional proteins is printed in multiple, identical arrays on a standard slide. After a blocking step, tagged protein samples are incubated with the arrays. The arrays are incubated with a fluorophore conjugated secondary antibodies. Signals are then visualized using a fluorescence laser scanner. For a list of laser scanner specifications, click here.

Protein-Protein Interactions - How it works

A panel of correctly folded, functional proteins is printed in multiple, identical arrays on a standard slide. After a blocking step, DNA samples are incubated with the arrays. The arrays are incubated with fluorophore conjugated polyclonal secondary antibodies. Signals are then visualized using a fluorescence laser scanner. For a list of laser scanner specifications, click here.

DNA-Protein Interaction - How it works

A panel of correctly folded, functional proteins is printed in multiple, identical arrays on a standard slide. Samples and controls are then added. Next, the slides are incubated with methylation cofactors. The arrays are incubated with fluorophore conjugated secondary antibodies that can detect the modifications. Signals are then visualized using a fluorescence laser scanner. For a list of laser scanner specifications, click here.

Methlyation Assay - How it works

Testing Services

Full Testing Service: Full testing services are available. Email our tech support team at techsupport@raybiotech.com to learn more.

Scanning Service: If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services.

For a list of laser scanner specifications, click here.

RayBiotech Protein Array slide scanning service is now FREE for a limited time only! Simply ship us your slide/s and we will scan the slide/s and send you the image for data extraction. Click to learn more.