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Instructions for Sending Samples

How do I prepare the sample?

Simply place each sample into a labeled vial. Freeze the sample right after collection if possible - you may even use a quickfreeze procedure. Please fill out the Sample Testing Service Form, including all necessary information with the package: contact info, sample info, and which cytokine antibody array system is to be used for testing.

Tips on Sample Preparation

How much sample volume is needed?

Note: It is preferable to send double the volumes listed below, just in case we need to repeat the experiment.

ELISA

100 µl minimum per replicate per target protein (eg, triplicate results for one target would require 300 µl per sample, and triplicate results for 2 target proteins would require 600 µl per sample)


Label-Based Antibody Arrays (L-Series) – membrane format

Serum or Plasma – minimum volume is 40 µl per sample

Cell Culture Supernatant – minimum volume is 5 mL per sample

Cell or Tissue Lysates - minimum volume is 500 µl (at ~2 mg/ml) per sample


Label-Based Antibody Arrays (L-Series) – glass slide format

Serum or Plasma – minimum volume is 40 µl per sample

Cell Culture Supernatant – minimum volume is 500 µL per sample

Cell or Tissue Lysates - minimum volume is 200-500 µl (at ~2 mg/ml) per sample

Note: If using cell culture supernatant samples, serum-free or low-serum containing media such as 0.2% FBS/FCS is preferred. For serum-containing media samples, sending an uncultured media blank sample is strongly recommended.


Sandwich-Based Membrane Antibody Arrays (C-Series)

Serum/Plasma/Cell Culture Supernatant/Other Body Fluids – minimum volume is 1 mL per sample.

Cell or Tissue Lysates – minimum volume is 200 µl (at 1-5 mg/ml) per sample.

Note: For the C2000 Arrays, send 2 times these amounts. For the C4000 Array, send 3 times these amounts


Sandwich-Based Glass Slide Antibody Arrays (G-Series and Quantibody Arrays)

Serum/Plasma/Cell Culture Supernatant/Other Body Fluids – minimum volume is 100 µL per sample per array

Cell or Tissue Lysates – minimum volume is 20 µl (at 1-5 mg/ml) per sample per array

Note: For G-1000/Q-1000 Arrays, send 2 times these amounts. For the G-2000/Q-2000 Arrays, send 3 times these amounts. For the Q-3000 Array, send 4 times these amounts. For the G-4000/Q-4000 Arrays, send 5 times these amounts. For the Q-5000 Array, send 6 times these amounts. For the Q-6000 Array, send 7 times these amounts. For the Q7000 Array, send 8 times these amounts. For the Q8000 Array, send 9 times these amounts.

High-Density Multiplex ELISA Service

ServiceSerum, Plasma & Lysates
Volume Needed		Cell Culture Media
Volume Needed
200 Human Biomarker Testing Service 250 µl per sample* 500 µl per sample
440 Human Biomarker Testing Service 700 µl per sample* 1.4 ml per sample
640 Human Biomarker Testing Service 1 ml per sample* 2 ml per sample
Kiloplex - 1000 Human Biomarker Testing Service 1.5 ml per sample* 3 ml per sample
200 Mouse Biomarker Testing Service 250 µl per sample* 500 µl per sample
67 Rat Biomarker Testing Service 100 µl per sample* 200 µl per sample

*The serum, plasma, and lysate volumes listed are before dilution. The RayBiotech service department will run at least a 2 fold dilution on these sample types unless otherwise requested by the customer.

Phosphorylation Antibody Arrays

Membrane-based – minimum amount of 1000 µg of total protein per sample.

Glass Slide-based – minimum amount of 100 µg of total protein per sample.

Note: These arrays are designed for use only with cell or tissue lysates.100 µl minimum per replicate per target protein (eg, triplicate results for one target would require 300 µl per sample, and triplicate results for 2 target proteins would require 600 µl per sample)

Glycobiology Arrays

ServiceSerum, Plasma & Cell Culture Media
Volume Needed		Cell Lysates
Volume Needed
Glycan 100 Testing Service 10 µL per sample
Note: For this array we will perform a 40X dilution.		 400 µL (at 50-500 µg/µL) per sample
Glycome 493& 507 Testing Services 400 µl per sample 400 µl per sample
Glycome 1000 800 µL per sample 800 µL per sample
Lectin 40 Testing Service 100 µl per sample 100µl (at 50-500 µg/µL) per sample

Protein Arrays

Human Allergen Array G1: Serum/Plasma/Cell Lysate/Cell Culture Media - 100 µL per sample

Mouse/Human Protein Array G2: Serum/Plasma/Cell Lysate/Cell Culture Media - 400 µL per sample

Ultrasensitive Biomarker Testing Service (Simoa™)

275 µl minimum final sample volume per target protein is required. Please note that Simoa testing samples are run in duplicate unless an alternate number of replicates is requested.

How do I send the samples?

Please include a printed copy of the Sample Testing Form with your shipment. Also, please indicate on the shipping label what type of service it is (C-Series Scanning Only, Quantibody Full Testing Service, ELISA Testing Service, etc.). Samples must be free of infections, such as HIV, Hepatitis B, or other communicable viruses, etc.

US Customers: Please include PO or credit card information for payment with your shipment.

International Customers: Please contact your local distributor for pricing and payment information.

Please inform us by e-mail when you send your samples (service@raybiotech.com). Please include a tracking number for the shipment, and an electronic copy of your completed Sample Testing Form.

Please send your samples frozen and on dry ice overnight shipping to:

RayBiotech, Inc.
Attn: Service Department - QUOTE#
3607 Parkway Lane, Suite 200
Norcross , GA 30092