Instructions for Sending Samples

How much sample volume is needed?

Note: It is preferable to send double the volumes listed below, just in case we need to repeat the experiment.

ELISA

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100 µl minimum per replicate per target protein (eg, triplicate results for one target would require 300 µl per sample, and triplicate results for 2 target proteins would require 600 µl per sample)

Label-Based Antibody Arrays (L-Series) – membrane format

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Serum or Plasma – minimum volume is 40 µl per sample

Cell Culture Supernatant – minimum volume is 5 mL per sample

Cell or Tissue Lysates - minimum volume is 500 µl (at ~2 mg/ml) per sample

Label-Based Antibody Arrays (L-Series) – glass slide format

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Serum or Plasma – minimum volume is 40 µl per sample

Cell Culture Supernatant – minimum volume is 500 µL per sample

Cell or Tissue Lysates - minimum volume is 200-500 µl (at ~2 mg/ml) per sample

Note: If using cell culture supernatant samples, serum-free or low-serum containing media such as 0.2% FBS/FCS is preferred. For serum-containing media samples, sending an uncultured media blank sample is strongly recommended.

Sandwich-Based Membrane Antibody Arrays (C-Series)

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Serum/Plasma/Cell Culture Supernatant/Other Body Fluids – minimum volume is 1 mL per sample.

Cell or Tissue Lysates – minimum volume is 200 µl (at 1-5 mg/ml) per sample.

Note: For the C2000 Arrays, send 2 times these amounts. For the C4000 Array, send 3 times these amounts

Sandwich-Based Glass Slide Antibody Arrays (G-Series and Quantibody Arrays)

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Serum/Plasma/Cell Culture Supernatant/Other Body Fluids – minimum volume is 100 µL per sample per array

Cell or Tissue Lysates – minimum volume is 20 µl (at 1-5 mg/ml) per sample per array

Note: For G-1000/Q-1000 Arrays, send 2 times these amounts. For the G-2000/Q-2000 Arrays, send 3 times these amounts. For the Q-3000 Array, send 4 times these amounts. For the G-4000/Q-4000 Arrays, send 5 times these amounts. For the Q-5000 Array, send 6 times these amounts. For the Q-6000 Array, send 7 times these amounts. For the Q7000 Array, send 8 times these amounts. For the Q8000 Array, send 9 times these amounts.

Quantitative Proteomics Services

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Service	Serum, Plasma, Lysates, & Cell Culture Media Volume Needed
Kiloplex - 1000 Human Biomarker Testing Service	1.5 ml per sample*
640 Human Biomarker Testing Service	1 ml per sample*
440 Human Biomarker Testing Service	700 µl per sample*
200 Human Biomarker Testing Service	250 µl per sample*
640 Mouse Biomarker Testing Service	Inquire
440 Mouse Biomarker Testing Service	Inquire
200 Mouse Biomarker Testing Service	250 µl per sample*
67 Rat Biomarker Testing Service	100 µl per sample*
50 Porcine Biomarker Testing Service	100 µl per sample*
30 Bovine Biomarker Testing Service	100 µl per sample*

*The volumes listed are before dilution. The RayBiotech service department will run at least a 2 fold dilution on these sample types unless otherwise requested by the customer.

Discovery Proteomics Services

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Service	Serum, Plasma, Lysates, & Cell Culture Media Volume Needed
2000 Human Biomarker Testing Service (Label-Based Platform)	40 µl per sample*
1000 Human Biomarker Testing Service (Label-Based Platform)	40 µl per sample*
507 Human Biomarker Testing Service (Label-Based Platform)	40 µl per sample*
500 Human Biomarker Testing Service (Label-Based Platform)	40 µl per sample*
493 Human Biomarker Testing Service (Label-Based Platform)	40 µl per sample*
1308 Mouse Biomarker Testing Service (Label-Based Platform)	50 µl per sample*
500 Mouse Biomarker Testing Service (Label-Based Platform)	40 µl per sample*
308 Mouse Biomarker Testing Service (Label-Based Platform)	40 µl per sample*
90 Rat Biomarker Testing Service (Label-Based Platform)	40 µl per sample*
Kiloplex - 1000 Human Biomarker Testing Service (Sandwich-Based Platform)	1.5 ml per sample*
640 Human Biomarker Testing Service (Sandwich-Based Platform)	1 ml per sample*
440 Human Biomarker Testing Service (Sandwich-Based Platform)	700 µl per sample*
200 Human Biomarker Testing Service (Sandwich-Based Platform)	250 µl per sample*
640 Mouse Biomarker Testing Service (Sandwich-Based Platform)	Inquire
400 Mouse Biomarker Testing Service (Sandwich-Based Platform)	Inquire
200 Mouse Biomarker Testing Service (Sandwich-Based Platform)	Inquire
67 Rat Biomarker Testing Service (Sandwich-Based Platform)	100 µl per sample*
50 Porcine Biomarker Testing Service (Sandwich-Based Platform)	100 µl per sample*
30 Bovine Biomarker Testing Service (Sandwich-Based Platform)	100 µl per sample*

*The volumes listed are before dilution. The RayBiotech service department will run at least a 2 fold dilution on these sample types unless otherwise requested by the customer.

Phosphorylation Antibody Arrays

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Membrane-based – minimum amount of 1000 µg of total protein per sample.

Glass Slide-based – minimum amount of 100 µg of total protein per sample.

Note: These arrays are designed for use only with cell or tissue lysates. 100 µl minimum per replicate per target protein (eg, triplicate results for one target would require 300 µl per sample, and triplicate results for 2 target proteins would require 600 µl per sample)

Glycobiology Arrays

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Service	Serum/Plasma Volume Needed	Cell Culture Media Volume Needed	Cell Lysates Volume Needed
Glycome 493 & 507 Testing Services	20 µl per sample	400 µl per sample	400 µl (at 1-2mg/ml) per sample
Glycome 1000	20 µl per sample	800 µl per sample	800 µl (at 1-2mg/ml) per sample
Lectin 40 Testing Service	20 µl per sample	100 µl per sample	100 µl (at 1-2mg/ml) per sample
Lectin 70 Testing Service	20 µl per sample	100 µl per sample	100 µl (at 1-2mg/ml) per sample
Lectin 95 Testing Service	20 µl per sample	100 µl per sample	100 µl (at 1-2mg/ml) per sample

Protein Arrays

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Human Allergen Array G1: Serum/Plasma/Cell Lysate/Cell Culture Media - 100 µL per sample

Mouse/Human Protein Array G2: Serum/Plasma/Cell Lysate/Cell Culture Media - 400 µL per sample

Ultrasensitive Biomarker Testing Service (Simoa^™)

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100 µl minimum final sample volume per target protein is required. Please note that Simoa testing samples are run in duplicate unless an alternate number of replicates is requested.

How do I send the samples?

Please include a printed copy of the Sample Testing Form with your shipment. Also, please indicate on the shipping label what type of service it is (C-Series Scanning Only, Quantibody Full Testing Service, ELISA Testing Service, etc.). Samples must be free of infections, such as HIV, Hepatitis B, or other communicable viruses, etc.

US Customers: Please include PO or credit card information for payment with your shipment.

International Customers: Please contact your local distributor for pricing and payment information.

Please inform us by e-mail when you send your samples ([email protected]). Please include a tracking number for the shipment, and an electronic copy of your completed Sample Testing Form.

Note: Please ship your samples overnight on Mondays through Wednesdays only. Samples should be frozen and on dry ice.

Shipping Address:

RayBiotech, Inc.
Attn: Service Department - QUOTE#
3607 Parkway Lane, Suite 200
Peachtree Corners, GA 30092

Sample Service Forms:

• Array
• ELISA
• Auto-Western
• Ultrasensitive Biomarker