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Less Sample, More Sensitivity

IQELISA - How It Works

The RayBio® Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

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  • Excellent for follow up/confirmation of antibody array data
  • Pre-coated 96 well plate
  • Strip plate format minimizes waste
  • Complete kit includes all necessary reagents
  • 10x more sensitive than ELISA
  • Reproducible and reliable
  • Optimized for serum, plasma, urine and cell culture medium
  • 1/10th the sample volume of an ELISA

Contents of Kit

  • 96-well pre-coated strip plate (12x8)
  • Wash Buffer Concentrate (20x)
  • Standards
  • Sample Diluent Buffers
  • Biotin-labeled Detection Reagent
  • Primer Solutions
  • PCR Master Mix
  • PCR Preparation buffer
  • Final Wash Buffer

Representative Data

IQELISA Standard Curve

Research Applications

  • Detection of quantitative protein levels in biological fluids
  • Validation of antibody array results
  • Validation of biomarker discovery studies

How It Works

IQELISA - How it works

RayBio® IQELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.