Human L182 Array, Membrane

RayBio® L-Series Human Obesity Antibody Array 182 Membrane Kit. Detects 182 Human Adipokines. Suitable for Serum, Plasma, Lysates, and Cell Culture Supernatants.

$709.00  
AAH-BLM-ADI-1-2
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Assay Format

Number of Targets Detected:
185
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Label-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2 or 4 Sample Kit

Protein Information

Gene Symbols:
ACE | ACE2 | ADAM17 | ADIPOQ | AGRP | AGT | ANGPT1 | ANGPT2 | ANGPT4 | ANGPTL1   View more

Product Features

  • High density arrays
  • Unbiased detection through direct biotin labeling of samples
  • Low sample consumption
  • High detection sensitivity
  • Same day result
  • Affordable and simple to use

Target Names

ACE / CD43, ACE-2, ACTH, ADFP, Adiponectin, Adipsin (Factor D), AgRP , AMPKa1 , Amylin, Angiopoietin 1, Angiopoietin 2, Angiotensinogen / Angiotensin II, Ang-like Factor, ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4 , Apelin Receptor , ApoB, ApoE, Axl, BDNF, bFGF, BMP-2 , BMP-3, BMP-3b / GDF-10, BMP-4 , BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA / ALK-3, BMPR-IB / ALK-6, BMPR-II, b-NGF, C3a des Arg, CART , CD137 (4-1BB), CD36, Clusterin, CNTF, C-peptide, CRP , Cystatin C, Dtk, EGF, EGFR, ENA-78, Endophin Beta, Epiregulin, E-selectin, ET-1 (Endothelin), FABP4, FAM3B, FAS / Apo-1, FGF-10, FGF-6, FSH , Galectin -1, Growth Hormone (Growth Hormone), Ghrelin, GITR, GITR Ligand, GLP-1 , Glucagon, Glut1, Glut2, Glut3, Glut5, Glutathione peroxidase 1, Glutathione peroxidase 3, GRO alpha, HCC4, HGF, HSD-1, ICAM1, IFN-gamma, IGF-1, IGF-1 sR, IGFBP-1, IGFBP-2, IGFBP-3, IGF-2, IL-1 R1, IL-1 R4, IL-1a, IL-1b, IL-1 ra, IL-6, IL-6 sR, IL-8, IL-10, IL-11, IL-12, IL-25 / IL-17E, INSL3, INSRR , Insulin, Insulin R (CD220), Leptin, Leptin R, LH (Luteinizing Hormone), LIF, LOX, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 alpha, MIP-1 beta, MIP-3b, MMP-2, MMP-9, MMP-11, MMP-19, MSHa , MSPa, Myostatin, NAIP, NeuroD1, Neurophilin-2, NGF R, NPY (Neuropeptide Y), Obestatin R (GPR-39), Orexin A, Orexin B, OSM, Osteocalcin, Osteonectin , Osteoprotegerin, PARC, PDGF, PDGF-AA, PDGF-AB, PDGF-C, PDGF-D, PEDF , Pentraxin-3, PPARg2 / NRIC3, Pref-1 , Prohibitin, Prolactin, PYY , RANTES, RBP4 , RELMb , Resistin, S100, S100 A8+A9, S100 A10, SAA , SDF-1, SEMA3A, Serotonin, Syndecan-3, TACE, TDAG51 , TECK, TGF alpha, TGF-b, Thrombospondin 1, Thrombospondin 2, Thrombospondin 4, TIMP-1, TIMP-2, TIMP-3, TIMP-4, Tissue factor (CD142), TLR2, TLR4, TNF alpha, sTNFRI, sTNFRII, TSG-6 , TSH , Vaspin, VCAM-1, VEGF-A, Visfatin/PBEF1, XEDAR

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Antibody Array Membranes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Detection Buffer C
  • Detection Buffer D
  • Wash Buffer 1
  • Wash Buffer 2
  • HRP-Conjugated Streptavidin Concentrate
  • Plastic Sheets
  • Spin Columns
  • Labeling Buffer
  • Lysis Buffer
  • Plastic Incubation Tray
Other Materials Required
  • Distilled or de-ionized water
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Pipettors, pipette tips and other common lab consumables
  • 2?5 ml tube
  • 15 ml conical collection tube
  • Tissue Paper, blotting paper or chromatography paper
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Purify Samples with Spin Column
  2. Biotinylate Samples
  3. Purify biotinylated samples using spin column
  4. Block array membranes
  5. Incubate with Samples
  6. Incubate with HRP-Conjugated Streptavidin
  7. Incubate with Detection Buffers
  8. Image with chemiluminescent imaging system
  9. Perform densitometry and analysis

Storage/Stability

For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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