Human L182 Array, Membrane

RayBio® L-Series Human Obesity Antibody Array 182 Membrane Kit. Detects 182 Human Adipokines. Suitable for Serum, Plasma, Lysates, and Cell Culture Supernatants.

Cell Culture Media Manual Serum, Plasma, Lysate Manual

$778.71
SDS
AAH-BLM-ADI-1A-2
+

Antigen Information

Gene Symbols:
ACE | ACE2 | ADAM17 | ADIPOQ | AGRP | AGT | ANGPT1 | ANGPT2 | ANGPT4 | ANGPTL1   View more

Assay Format

Number of Targets Detected:
185
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Label-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2 or 4 Sample Kit

Product Feature

  • High density arrays
  • 182 Human proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use

Target Name

ACE / CD43, ACE-2, ACTH, ADFP, Adiponectin, Adipsin (Factor D), AgRP , AMPKa1 , Amylin, Angiopoietin 1, Angiopoietin 2, Angiotensinogen / Angiotensin II, Ang-like Factor, ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4 , Apelin Receptor , ApoB, ApoE, Axl, BDNF, bFGF, BMP-2 , BMP-3, BMP-3b / GDF-10, BMP-4 , BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA / ALK-3, BMPR-IB / ALK-6, BMPR-II, b-NGF, C3a des Arg, CART , CD137 (4-1BB), CD36, Clusterin, CNTF, C-peptide, CRP , Cystatin C, Dtk, EGF, EGFR, ENA-78, Endophin Beta, Epiregulin, E-selectin, ET-1 (Endothelin), FABP4, FAM3B, FAS / Apo-1, FGF-10, FGF-6, FSH , Galectin -1, Growth Hormone (Growth Hormone), Ghrelin, GITR, GITR Ligand, GLP-1 , Glucagon, Glut1, Glut2, Glut3, Glut5, Glutathione peroxidase 1, Glutathione peroxidase 3, GRO alpha, HCC4, HGF, HSD-1, ICAM1, IFN-gamma, IGF-1, IGF-1 sR, IGFBP-1, IGFBP-2, IGFBP-3, IGF-2, IL-1 R1, IL-1 R4, IL-1a, IL-1b, IL-1 ra, IL-6, IL-6 sR, IL-8, IL-10, IL-11, IL-12, IL-25 / IL-17E, INSL3, INSRR , Insulin, Insulin R (CD220), Leptin, Leptin R, LH (Luteinizing Hormone), LIF, LOX, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 alpha, MIP-1 beta, MIP-3b, MMP-2, MMP-9, MMP-11, MMP-19, MSHa , MSPa, Myostatin, NAIP, NeuroD1, Neurophilin-2, NGF R, NPY (Neuropeptide Y), Obestatin R (GPR-39), Orexin A, Orexin B, OSM, Osteocalcin, Osteonectin , Osteoprotegerin, PARC, PDGF, PDGF-AA, PDGF-AB, PDGF-C, PDGF-D, PEDF , Pentraxin-3, PPARg2 / NRIC3, Pref-1 , Prohibitin, Prolactin, PYY , RANTES, RBP4 , RELMb , Resistin, S100, S100 A8+A9, S100 A10, SAA , SDF-1, SEMA3A, Serotonin, Syndecan-3, TACE, TDAG51 , TECK, TGF alpha, TGF-b, Thrombospondin 1, Thrombospondin 2, Thrombospondin 4, TIMP-1, TIMP-2, TIMP-3, TIMP-4, Tissue factor (CD142), TLR2, TLR4, TNF alpha, sTNFRI, sTNFRII, TSG-6 , TSH , Vaspin, VCAM-1, VEGF-A, Visfatin/PBEF1, XEDAR

Target Species

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Human Obesity Antibody Array Membranes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Plastic Sheets
  • Floating Dialysis Rack
  • Wash Buffer 1
  • Wash Buffer 2
  • Spin Columns
  • Plastic Incubation Tray
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4 and Na2HPO4
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • Pipettors, pipette tips and other common lab consumables
  • 2~5 ml tube
  • 50 ml conical collection tube
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated Samples in PBS
  4. Block array membranes
  5. Incubate with Samples
  6. Incubate with HRP-Conjugated Streptavidin
  7. Incubate with Detection Buffers
  8. Image with chemiluminescent imaging system
  9. Perform densitometry and analysis

Storage/Stability

For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Analysis Tool Bundle Discount

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70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $1,013.03
Price for all $849.01

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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