Mouse L308 Array, Membrane

$918.09
SDS

RayBio® L-Series Mouse Antibody Array 308 Membrane Kit. Detects 308 Mouse Proteins. Suitable for Serum, Plasma, Lysates, and Cell Culture Supernatants.

Cell Culture Media Manual Serum, Plasma, Lysate Manual

AAM-BLM-1A-2
+

Antigen Information

Gene Symbol:
ACVR1B | ADIPOQ | AGER | AGRP | ALCAM | ANGPTL2 | ANGPTL3 | AREG | ARTN | AXL   View more

Assay Format

Number of Targets Detected:
308
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Label-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2 or 4 Sample Kit

Product Feature

  • High density arrays
  • 308 Mouse proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use

Target Name

6Ckine, Activin A, Activin C, Activin RIB / ALK-4, Adiponectin / Acrp30, AgRP, ALCAM, Angiopoietin-like 2, Angiopoietin-like 3, AREG (Amphiregulin), Artemin, Axl, bFGF, B7-1/CD80, BAFF R / TNFRSF13C, BCMA / TNFRSF17, beta-Catenin, BLC, BTC (Betacellulin), Cardiotrophin-1, CCL1 / I-309 / TCA-3, CCL28, CCL4 / MIP-1 beta, CCL7 / MCP-3 / MARC, CCL8 / MCP-2, CCR10, CCR3, CCR4, CCR6, CCR7, CCR9, CD11b, CD14, CRP, CD27 / TNFRSF7, CD27 Ligand / TNFSF7, CD30, CD30 L, CD40, CD40 Ligand / TNFSF5, Cerberus 1, Chordin-Like 2, Coagulation Factor III / Tissue Factor, Common gamma Chain / IL-2 R gamma, CRG-2, Cripto, Crossveinless-2, Cryptic, Csk, CTACK , CTLA-4 / CD152, CXCL14 / BRAK, CXCL16, CXCR2 / IL-8 RB, CXCR3, CXCR4, CXCR6, DAN, Decorin, DKK-1, Dkk-3, Dkk-4, DPPIV / CD26, DR3 / TNFRSF25, Dtk, EDAR, EGF R, EG-VEGF / PK1, Endocan, Endoglin / CD105, Endostatin, Eotaxin, Eotaxin-2, Epigen, Epiregulin, Erythropoietin (EPO), E-Selectin, FADD, FAM3B, Fas / TNFRSF6, Fas Ligand, FCrRIIB / CD32b, FGF R3, FGF R4, FGF R5 beta, FGF-21, Fit-3 Ligand, FLRG (Follistatin), Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-6, Frizzled-7, Galectin-3, G-CSF, GDF-1, GDF-3, GDF-5, GDF-8, GDF-9, GFR alpha-2 / GDNF R alpha-2, GFR alpha-3 / GDNF R alpha-3, GFR alpha-4 / GDNF R alpha-4, GITR, GITR Ligand / TNFSF18, Glut2, GM-CSF, Granzyme B, Granzyme D, Granzyme G, Gremlin, Growth Hormone R, HGF R, HGF, HVEM / TNFRSF14, ICAM-1, ICAM-2 / CD102, ICAM-5, ICK, IFN-alpha / beta R1, IFN-alpha / beta R2, IFN-beta, IFN-gamma , IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGFBP-rp1 / IGFBP-7, IGF-I, IGF-II, IL-1 alpha, IL-1 beta, IL-1 R4 / ST2, IL-1 R6 / IL-1 R rp2, IL-1 R9, IL-1 RI, IL-1 RII, IL-2, IL-2 R alpha, IL-2 R beta, IL-3, IL-3 R alpha, IL-3 R beta, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-9, IL-9 R, IL-10, IL-10 R alpha, IL-11, IL-12 p40/p70, IL-12 p70, IL-12 R beta 1, IL-13, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 R alpha/IL-1 R5, IL-20, IL-20 R alpha, IL-21, IL-21 R, IL-22, IL-22BP, IL-23, IL-23 R, IL-24, IL-27, IL-28 / IFN-lambda, IL-31, IL-31 RA, Insulin, Integrin beta 2 / CD18, I-TAC, KC, Kremen-1, Kremen-2, Lefty-1, Leptin R, LEPTIN(OB), LIF, LIGHT / TNFSF14, LIX, LRP-6, L-Selectin, Lungkine, Lymphotactin, Lymphotoxin beta R / TNFRSF3, MAdCAM-1, MCP-1, MCP-5, M-CSF, MDC, MFG-E8, MFRP, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta , MMP-2, MMP-3, MMP-9, MMP-12, MMP-14 / LEM-2, MMP-24 / MT5-MMP, Neuregulin-3 / NRG3, Neurturin, NGF R / TNFRSF16, NOV / CCN3, Osteoactivin / GPNMB, Osteopontin, Osteoprotegerin, OX40 Ligand / TNFSF4, PDGF C, PDGF R alpha, PDGF R beta, Pentraxin3 / TSG-14, PF-4, PlGF-2, Progranulin, Prolactin, P-Selectin, RAGE, RANTES, RELM beta, Resistin, S100A10, SCF, SCF R / c-kit, SDF-1, Serum Amyloid A1, Shh-N, SIGIRR, SLPI, Soggy-1, SPARC, Spinesin Ectodomain, TACI / TNFRSF13B, TARC, TCA-3, TCCR / WSX-1, TECK, TFPI, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta RI / ALK-5, TGF-beta RII, Thrombospondin, Thymus Chemokine-1, Tie-2, TIMP-1 , TIMP-2, TIMP-4, TL1A / TNFSF15, TLR1, TLR2, TLR3, TLR4, TMEFF1 / Tomoregulin-1, TNF RI / TNFRSF1A, TNF RII, TNF-alpha, TNF-beta / TNFSF1B, TPO, TRAIL / TNFSF10, TRAIL R2 / TNFRSF10B, TRANCE / TNFSF11, TREM-1, TROY, TSLP, TSLP R, TWEAK / TNFSF12, TWEAK R / TNFRSF12, Ubiquitin, uPAR, Urokinase, VCAM-1, VE-Cadherin, VEGF, VEGF R1, VEGF R2, VEGF R3, VEGF-B, VEGF-C, VEGF-D, WIF-1, WISP-1 / CCN4

Target Species

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Mouse 308 Antibody Array Membranes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Plastic Sheets
  • Floating Dialysis Rack
  • Wash Buffer 1
  • Wash Buffer 2
  • Spin Columns
  • Plastic Incubation Tray
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4 and Na2HPO4
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • Pipettors, pipette tips and other common lab consumables
  • 2~5 ml tube
  • 50 ml conical collection tube
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated Samples in PBS
  4. Block array membranes
  5. Incubate with Samples
  6. Incubate with HRP-Conjugated Streptavidin
  7. Incubate with Detection Buffers
  8. Image with chemiluminescent imaging system
  9. Perform densitometry and analysis

Storage/Stability

For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Torre-Minguela C., Barbera-cremades M., Gomex A., et al. Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process. Scientific Reports 6, Article number: 22586 (2016) doi:10.1038/srep22586
    Species: Mouse
    Sample type: Conditioned Media
  2. Wang Y., Wang D., Wu J., et al. Cinnabar induces renal inflammation and fibrogenesis in rats. Biomed Res Int. 2015;2015:280958. doi: 10.1155/2015/280958.
    Species: Mouse
    Sample type: Tissue Lysate
  3. He S., Zhang H., Liu S., et al. gd T cells regulate the expression of cytokines but not the manifestation of fungal keratitis. Exp Eye Res. 2015 Jun;135:93-101. doi: 10.1016/j.exer.2015.03.022.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Watanabe K., Uemura K., Asada M., et al. The participation of insulin-like growth factor-binding protein 3 released by astrocytes in the pathology of Alzheimer's disease. Mol Brain. 2015 Dec 4;8(1):82. doi: 10.1186/s13041-015-0174-2.
    Species: Mouse
    Sample type: Conditioned Media
  5. Amano H., Murata K., Matsunaga H., Tanaka K., Yoshioka K., Kobayashi T., et al. p38 Mitogen-activated protein kinase accelerates emphysema in mouse model of chronic obstructive pulmonary disease. Journal of Receptors and Signal Transduction, March 5, 2014. (doi:10.3109/10799893.2014.896380) [Epub Ahead of Print]
    Species: Mouse
    Sample type: BAL Fluid
  6. Ma W., et al. Twist 1 regulates the expression of PPAR? during hormone-induced 3T3-L1 preadipocyte differentiation: a possible role in obesity and associated diseases. Lipids in Health and Disease 2014, 13:132. doi:10.1186/1476-511X-13-132
    Species: Mouse
    Sample type: Conditioned Media
  7. Tanaka K., et al. Therapeutic effect of lung mixed culture-derivedepithelial cells on lung fibrosis. Laboratory Investigation advance online publication 8 September 2014; doi: 10.1038/labinvest.2014.109
    Species: Mouse
    Sample type: BAL Fluid
  8. Wolford C., McConoughey S., Jalgaonkar S., et al. Transcription factor ATF3 links host adaptive response to breast cancer metastasis. J Clin Invest. 2013 Jul 1; 123(7): 2893-2906. doi: 10.1172/JCI64410
    Species: Mouse
    Sample type: Plasma
  9. Fleuren, Wilco WM, et al. "Prednisolone induces the Wnt signalling pathway in 3T3-L1 adipocytes." Archives of physiology and biochemistry 119.2 (2013): 52-64.
    Species: Mouse
    Sample type: Conditioned Media
  10. Schackmann, Ron CJ, et al. "Loss of p120-catenin induces metastatic progression of breast cancer by inducing anoikis resistance and augmenting growth factor receptor signaling." Cancer research 73.15 (2013): 4937-4949.
    Species: Mouse
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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