Human L507 Array, Glass Slide

RayBio® L-Series Human Antibody Array 507 Glass Slide Kit. Detects 507 Human Proteins. Suitable for serum, plasma and cell culture supernatants.

$1,431.68
AAH-BLG-1-4
+

Antigen Information

Gene Symbols:
ACKR2 | ACVR1 | ACVR1B | ACVR2A | ACVR2B | ADIPOQ | AGER | AGRP | ALCAM | ANG   View more

Assay Format

Number of Targets Detected:
507
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Label-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit
NEED HELP RUNNING THIS ASSAY? 50% Off Discovery Proteomics Services Through december REQUEST QUOTE | MORE INFO

Product Feature

  • High density arrays
  • 507 Human proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use

Target Name

6Ckine, Activin A, Activin B, Activin C, Activin RIA (ALK-2), Activin RIB (ALK-4), Activin RII A/B, Activin RIIA, Adiponectin (Acrp30), AgRP, ALCAM, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiopoietin-like 1, Angiopoietin-like 2, Angiopoietin-like Factor, Angiostatin, APJ, APRIL, AR (Amphiregulin), Artemin, Axl, B7-1 (CD80), BAFF R (TNFRSF13C), BCMA (TNFRSF17), BD-1, BDNF, beta-Catenin, BAX, beta-NGF, BIK, BLC (BCA-1/CXCL13), BMP-2, BMP-3, BMP-3b (GDF-10), BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA (ALK-3), BMPR-IB (ALK-6), BMPR-II, BTC, Cardiotrophin-1 (CT-1), CCL14 (HCC-1/HCC-3), CCL28 (VIC), CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD27 (TNFRSF7), CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5/CD154), CD 163, Cerberus 1, Chem R23, Chordin-Like 1, Chordin-Like 2, CLC, CNTF, CNTF R alpha, Coagulation Factor III (Tissue Factor), CRIM 1, Cripto-1, CRTH-2, Cryptic, Csk, CTACK (CCL27), CTGF (CCN2), CTLA-4 (CD152), CV-2 (Crossveinless-2), CXCL14 (BRAK), CXCL16, CXCR1 (IL-8 RA), CXCR2 (IL-8 RB), CXCR3, CXCR4 (fusin), CXCR5 (BLR-1), CXCR6, D6, DAN, DANCE, DcR3 (TNFRSF6B), Decorin, Dkk-1, Dkk-3, Dkk-4, DR3 (TNFRSF25), DR6 (TNFRSF21), Dtk, EDA-A2, EDAR, EDG-1, EGF, EGF R (ErbB1), EG-VEGF (PK1), EMAP-II, ENA-78, Endocan, Endoglin (CD105), Endostatin, Endothelin, EN-RAGE, Eotaxin (CCL11), Eotaxin-2 (MPIF-2), Eotaxin-3 (CCL26), Epiregulin, ErbB2, ErbB3, ErbB4, Erythropoietin, E-Selectin, FADD, FAM3B, Fas (TNFRSF6), Fas Ligand, FGF Basic, FGF-BP, FGF R3, FGF R4, FGF R5, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-8, FGF-9, FGF-10 (KGF-2), FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Galectin-3, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, G-CSF R (CD 114), GDF1, GDF3, GDF5, GDF8, GDF9, GDF11, GDF-15, GDNF, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, GITR (TNFRF18), GITR Ligand (TNFSF18), Glucagon, Glut1, Glut2, Glut3, Glut5, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, Granzyme A, GREMLIN, GRO, GRO-a, Growth Hormone (GH), Growth Hormone R (GHR), HB-EGF, HCC-4 (CCL16), HCR (CRAM-A/B), Hepassocin, GLO-1, HGF, HGFR, HRG-alpha, HRG-beta 1, HVEM (TNFRSF14), I-309, ICAM-1, ICAM-2, ICAM-3 (CD50), ICAM-5, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGFBP-rp1 (IGFBP-7), IGF-I, IGF-I SR, IGF-II, IGF-II R, IL-1 alpha, IL-1 beta, IL-1 F5 (FIL1delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 F10 (IL-1HY2), IL-1 R3 (IL-1 R AcP), IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 R8, IL-1 R9, IL-1 ra, IL-1 sRI, IL-1 sRII, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma, IL-3, IL-3 R alpha, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 BPa, IL-18 R alpha (IL-1 R5), IL-18 R beta (AcPL), IL-19, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 BP, IL-22 R, IL-23, IL-23 R, IL-24, IL-26, IL-27, IL-28A, IL-29, IL-31, IL-31 RA, BACE-1, FACX, Insulin, Insulin R, Insulysin (IDE), IP-10, I-TAC (CXCL11), Kininostatin (kininogen), Kremen-1, Kremen-2, Latent TGF-beta bp1, LBP, Lck, LECT2, Lefty - A, Leptin (OB), Leptin R, LFA-1 alpha, LIF, LIF R alpha, LIGHT (TNFSF14), Lipocalin-1, Lipocalin-2, LRP-1, LRP-6, L-Selectin (CD62L), Lymphotactin (XCL1), Lymphotoxin beta (TNFSF3), Lymphotoxin beta R (TNFRSF3), MAC-1, MCP-1, MCP-2, MCP-3, MCP-4 (CCL13), M-CSF, M-CSF R, MDC, MFG-E8, MFRP, MICA, MIF, MIG, MIP-1a, MIP-1b, MIP-1d, MIP 2, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11 (Stromelysin-3), MMP-12, MMP-13, MMP-14, MMP-15, MMP-16 (MT3-MMP), MMP-19, MMP-20, MMP-24 (MT5-MMP), MMP-25 (MT6-MMP), MSP alpha Chain, Musk, NAP-2, NCAM-1 (CD56), Neuritin, NeuroD1, Neuropilin-2, Neurturin, NGF R, Nidgen-1, NOV (CCN3), NrCAM, NRG1 Isoform GGF2, NRG2, NRG3, NT-3, NT-4, Orexin A, Orexin B, OSM, Osteoactivin (GPNMB), Osteocrin, Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), PARC (CCL18), PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PECAM-1 (CD31), Pentraxin3 (TSG-14), Persephin, PF4 (CXCL4), PlGF, PLUNC, Pref-1, Progranulin, Prolactin, P-selectin, RAGE, RANK (TNFRSF11A), RANTES, RELM beta, RELT (TNFRSF19L), ROBO4, S100 A8/A9, S100A10, SAA, SCF, SCF R (CD117), SDF-1 (CXCL12), sFRP-1, sFRP-3, sFRP-4, sgp130, SIGIRR, Siglec-5 (CD170), Siglec-9, SLPI, Smad 1, Smad 4, Smad 5, Smad 7, Smad 8, Prdx6, Soggy-1, Sonic Hedgehog (Shh N-terminal), SPARC, Spinesin, TACI (TNFRSF13B), Tarc, TCCR (WSX-1), TECK (CCL25), TFPI, TGF-alpha, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta 5, TGF-beta RI (ALK-5), TGF-beta RII, Grb2, TGF-beta RIII, Thrombopoietin (TPO), TPX, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A (TNFSF15), TLR1, TLR2, TLR3, TLR4, TMEFF1 (Tomoregulin-1), TMEFF2, TNF-alpha, TNF-beta, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRADD, TRAIL (TNFSF10), TRAIL R1 (DR4/TNFRSF10A), TRAIL R2 (DR5/TNFRSF10B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRANCE, TREM-1, TROY (TNFRSF19), TSG-6, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), Ubiquitin+1, uPA, uPAR, Vasorin, VCAM-1 (CD106), VE-Cadherin, VEGF, VEGF R2 (KDR), VEGF R3, VEGF-B, VEGF-C, VEGF-D, VEGI (TNFSF15), WIF-1, WISP-1 (CCN4), XEDAR

Target Species

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Human 507 Antibody Array Glass Slide(s)
  • Dialysis Tubes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Streptavidin-Conjugated Fluor
  • Adhesive Plastic Strips
  • Serum Buffer
  • Floating Dialysis Rack
  • 30 ml Centrifuge Tube
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4 and Na2HPO4
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • 1 ml tube
  • Pipettors, pipette tips and other common lab consumables
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated samples using spin column
  4. Dry the glass slide
  5. Block array surface
  6. Incubate with Samples
  7. Incubate with Streptavidin-Conjugated Fluor
  8. Disassemble the glass slide
  9. Scan with a gene microarray laser scanner
  10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Lee N., Park S., Kwon S., et al. Agouti Related Peptide Secreted Via Human Mesenchymal Stem Cells Upregulates Proteasome Activity in an Alzheimer’s Disease Model. Sci Rep. 2017; 7: 39340. Jan 4. doi: 10.1038/srep39340
    Species: Human
    Sample type: Conditioned Media
  2. Meltzer S., Kalanxhi E., Hektoen H., et al. Systemic release of osteoprotegerin during oxaliplatincontaining induction chemotherapy and favorable systemic outcome of sequential radiotherapy in rectal cancer. Oncotarget. 2016 Apr 26. doi: 10.18632/oncotarget.8995
    Species: Human
    Sample type: Serum
  3. Lu P., Zheng D., Fang C., et al. Cytokines in cerebrospinal fluid of neurosyphilis patients: Identification of Urokinase plasminogen activator using antibody microarrays. J Neuroimmunol. 2016 Apr 15;293:39-44. doi: 10.1016/j.jneuroim.2015.12.010.
    Species: Human
    Sample type: CSF
  4. Williams K., Killebrew D., Clary G., Meeker R. Opposing Effects of NGF and proNGF on HIV Induced Macrophage Activation. J Neuroimmune Pharmacol. 2016 Mar;11(1):98-120. doi: 10.1007/s11481-015-9631-z
    Species: Human
    Sample type: Conditioned Media
  5. Qu X., Zhou J., Wang T., et al. MiR-30a inhibits Th17 differentiation and demyelination of EAE mice by targeting the IL-21R. Brain Behav Immun. 2016 Oct;57:193-9. doi: 10.1016/j.bbi.2016.03.016.
    Species: Human
    Sample type: Serum
  6. Williams K., Killebrew D., Clary G., et al. Differential regulation of macrophage phenotype by mature and pro-nerve growth factor. J Neuroimmunol. 2015 Aug 15;285:76-93. doi: 10.1016/j.jneuroim.2015.05.016
    Species: Human
    Sample type: Conditioned Media
  7. Zhang Y., Liang X., Liao S., et al. Potent Paracrine Effects of human induced Pluripotent Stem Cell-derived Mesenchymal Stem Cells Attenuate Doxorubicin-induced Cardiomyopathy. Sci Rep. 2015 Jun 9;5:11235. doi: 10.1038/srep11235.
    Species: Human
    Sample type: Conditioned Media
  8. Soria-Valles C., Osorio F., Gutierrez-Fernandez A., et al. NF-?B activation impairs somatic cell reprogramming in ageing. Nat Cell Biol. 2015 Aug;17(8):1004-13. doi: 10.1038/ncb3207
    Species: Human
    Sample type: Conditioned Media
  9. Torres C., et al. Serum Cytokine Profile in Patients With Pancreatic Cancer. Pancrease 2014 Oct;43(7):1042-9. doi: 10.1097/MPA.0000000000000155.
    Species: Human
    Sample type: Serum
  10. Bailey K., Laine J., Rager J., Sebastian E., Olshan A., Smeester L., et al. Prenatal arsenic exposure and shifts in the newborn proteome: inter-individual differences in tumor necrosis factor (TNF)-responsive signaling. Toxicol. Sci. (2014) doi: 10.1093/toxsci/kfu053 [Epub Ahead of Print]
    Species: Human
    Sample type: Conditioned Media
Array worked very well. I used the 4 sample set, followed the directions, and obtained very usable results.
That said, the array is still finicky. The results obtained from each sample spot has a relatively large variation, and many of the spots have tails. However, the results don't seem to change, whether or not you include the tails in the quantification. The individual spots on the array could be controlled/printed better.

University of California, Berkeley
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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