Human L507 Array, Membrane

RayBio® L-Series Human Antibody Array 507 Membrane Kit. Detects 507 Human Proteins. Suitable for Serum, Plasma, Lysates, and Cell Culture Supernatants.

Cell Culture Media Manual Serum, Plasma, Lysate Manual

$1,076.66  
AAH-BLM-1A-2
+

Antigen Information

Gene Symbols:
ACKR2 | ACVR1 | ACVR1B | ACVR2A | ACVR2B | ADIPOQ | AGER | AGRP | ALCAM | ANG   View more

Assay Format

Number of Targets Detected:
507
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Label-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2 or 4 Sample Kit

Product Feature

  • High density arrays
  • 507 Human proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use

Target Name

6Ckine, Activin A, Activin B, Activin C, Activin RIA (ALK-2), Activin RIB (ALK-4), Activin RII A/B, Activin RIIA, Adiponectin (Acrp30), AgRP, ALCAM, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiopoietin-like 1, Angiopoietin-like 2, Angiopoietin-like Factor, Angiostatin, APJ, APRIL, AR (Amphiregulin), Artemin, Axl, B7-1 (CD80), BAFF R (TNFRSF13C), BCMA (TNFRSF17), BD-1, BDNF, beta-Catenin, BAX, beta-NGF, BIK, BLC (BCA-1/CXCL13), BMP-2, BMP-3, BMP-3b (GDF-10), BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA (ALK-3), BMPR-IB (ALK-6), BMPR-II, BTC, Cardiotrophin-1 (CT-1), CCL14 (HCC-1/HCC-3), CCL28 (VIC), CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD27 (TNFRSF7), CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5/CD154), CD 163, Cerberus 1, Chem R23, Chordin-Like 1, Chordin-Like 2, CLC, CNTF, CNTF R alpha, Coagulation Factor III (Tissue Factor), CRIM 1, Cripto-1, CRTH-2, Cryptic, Csk, CTACK (CCL27), CTGF (CCN2), CTLA-4 (CD152), CV-2 (Crossveinless-2), CXCL14 (BRAK), CXCL16, CXCR1 (IL-8 RA), CXCR2 (IL-8 RB), CXCR3, CXCR4 (fusin), CXCR5 (BLR-1), CXCR6, D6, DAN, DANCE, DcR3 (TNFRSF6B), Decorin, Dkk-1, Dkk-3, Dkk-4, DR3 (TNFRSF25), DR6 (TNFRSF21), Dtk, EDA-A2, EDAR, EDG-1, EGF, EGF R (ErbB1), EG-VEGF (PK1), EMAP-II, ENA-78, Endocan, Endoglin (CD105), Endostatin, Endothelin, EN-RAGE, Eotaxin (CCL11), Eotaxin-2 (MPIF-2), Eotaxin-3 (CCL26), Epiregulin, ErbB2, ErbB3, ErbB4, Erythropoietin, E-Selectin, FADD, FAM3B, Fas (TNFRSF6), Fas Ligand, FGF Basic, FGF-BP, FGF R3, FGF R4, FGF R5, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-8, FGF-9, FGF-10 (KGF-2), FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Galectin-3, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, G-CSF R (CD 114), GDF1, GDF3, GDF5, GDF8, GDF9, GDF11, GDF-15, GDNF, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, GITR (TNFRF18), GITR Ligand (TNFSF18), Glucagon, Glut1, Glut2, Glut3, Glut5, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, Granzyme A, GREMLIN, GRO, GRO-a, Growth Hormone (GH), Growth Hormone R (GHR), HB-EGF, HCC-4 (CCL16), HCR (CRAM-A/B), Hepassocin, GLO-1, HGF, HGFR, HRG-alpha, HRG-beta 1, HVEM (TNFRSF14), I-309, ICAM-1, ICAM-2, ICAM-3 (CD50), ICAM-5, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGFBP-rp1 (IGFBP-7), IGF-I, IGF-I SR, IGF-II, IGF-II R, IL-1 alpha, IL-1 beta, IL-1 F5 (FIL1delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 F10 (IL-1HY2), IL-1 R3 (IL-1 R AcP), IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 R8, IL-1 R9, IL-1 ra, IL-1 sRI, IL-1 sRII, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma, IL-3, IL-3 R alpha, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 BPa, IL-18 R alpha (IL-1 R5), IL-18 R beta (AcPL), IL-19, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 BP, IL-22 R, IL-23, IL-23 R, IL-24, IL-26, IL-27, IL-28A, IL-29, IL-31, IL-31 RA, BACE-1, FACX, Insulin, Insulin R, Insulysin (IDE), IP-10, I-TAC (CXCL11), Kininostatin (kininogen), Kremen-1, Kremen-2, Latent TGF-beta bp1, LBP, Lck, LECT2, Lefty - A, Leptin (OB), Leptin R, LFA-1 alpha, LIF, LIF R alpha, LIGHT (TNFSF14), Lipocalin-1, Lipocalin-2, LRP-1, LRP-6, L-Selectin (CD62L), Lymphotactin (XCL1), Lymphotoxin beta (TNFSF3), Lymphotoxin beta R (TNFRSF3), MAC-1, MCP-1, MCP-2, MCP-3, MCP-4 (CCL13), M-CSF, M-CSF R, MDC, MFG-E8, MFRP, MICA, MIF, MIG, MIP-1a, MIP-1b, MIP-1d, MIP 2, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11 (Stromelysin-3), MMP-12, MMP-13, MMP-14, MMP-15, MMP-16 (MT3-MMP), MMP-19, MMP-20, MMP-24 (MT5-MMP), MMP-25 (MT6-MMP), MSP alpha Chain, Musk, NAP-2, NCAM-1 (CD56), Neuritin, NeuroD1, Neuropilin-2, Neurturin, NGF R, Nidgen-1, NOV (CCN3), NrCAM, NRG1 Isoform GGF2, NRG2, NRG3, NT-3, NT-4, Orexin A, Orexin B, OSM, Osteoactivin (GPNMB), Osteocrin, Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), PARC (CCL18), PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PECAM-1 (CD31), Pentraxin3 (TSG-14), Persephin, PF4 (CXCL4), PlGF, PLUNC, Pref-1, Progranulin, Prolactin, P-selectin, RAGE, RANK (TNFRSF11A), RANTES, RELM beta, RELT (TNFRSF19L), ROBO4, S100 A8/A9, S100A10, SAA, SCF, SCF R (CD117), SDF-1 (CXCL12), sFRP-1, sFRP-3, sFRP-4, sgp130, SIGIRR, Siglec-5 (CD170), Siglec-9, SLPI, Smad 1, Smad 4, Smad 5, Smad 7, Smad 8, Prdx6, Soggy-1, Sonic Hedgehog (Shh N-terminal), SPARC, Spinesin, TACI (TNFRSF13B), Tarc, TCCR (WSX-1), TECK (CCL25), TFPI, TGF-alpha, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta 5, TGF-beta RI (ALK-5), TGF-beta RII, Grb2, TGF-beta RIII, Thrombopoietin (TPO), TPX, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A (TNFSF15), TLR1, TLR2, TLR3, TLR4, TMEFF1 (Tomoregulin-1), TMEFF2, TNF-alpha, TNF-beta, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRADD, TRAIL (TNFSF10), TRAIL R1 (DR4/TNFRSF10A), TRAIL R2 (DR5/TNFRSF10B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRANCE, TREM-1, TROY (TNFRSF19), TSG-6, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), Ubiquitin+1, uPA, uPAR, Vasorin, VCAM-1 (CD106), VE-Cadherin, VEGF, VEGF R2 (KDR), VEGF R3, VEGF-B, VEGF-C, VEGF-D, VEGI (TNFSF15), WIF-1, WISP-1 (CCN4), XEDAR

Target Species

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Human 507 Antibody Array Membranes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • HRP-Conjugated Streptavidin Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • Plastic Sheets
  • Floating Dialysis Rack
  • Dialysis Vials
  • Wash Buffer 1
  • Wash Buffer 2
  • Spin Columns
  • Plastic Incubation Tray
  • (Lysate specific kit) Lysis Buffer
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4 and Na2HPO4
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • Pipettors, pipette tips and other common lab consumables
  • 2~5 ml tube
  • 50 ml conical collection tube
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated Samples in PBS
  4. Block array membranes
  5. Incubate with Samples
  6. Incubate with HRP-Conjugated Streptavidin
  7. Incubate with Detection Buffers
  8. Image with chemiluminescent imaging system
  9. Perform densitometry and analysis

Storage/Stability

For best results, store Box 1 frozen at -20^^C and Box 2 at 4^^C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4^^C for no more than 3 months.
  1. Hu, Li, et al. "Side-by-side comparison of the biological characteristics of human umbilical cord and adipose tissue-derived mesenchymal stem cells."?BioMed research international?2013 (2013).
    Species: Human
    Sample type: Conditioned Media
  2. Cha, Dong Min, et al. "Comparative analysis of aqueous humor cytokine levels between patients with exudative age-related macular degeneration and normal controls."?Investigative ophthalmology & visual science?54.10 (2013): 7038-7044.
    Species: Human
    Sample type: aqueous humor
  3. Zhao, Jiajia, et al. "The effects of cytokines in adipose stem cell-conditioned medium on the migration and proliferation of skin fibroblasts in vitro."?BioMed research international?2013 (2013).
    Species: Human
    Sample type: Conditioned Media
  4. Sugitani, Sou, et al. "The potential neuroprotective effect of human adipose stem cells conditioned medium against light-induced retinal damage."?Experimental eye research?116 (2013): 254-264.
    Species: Human
    Sample type: Conditioned Media
  5. Bandi S, Cheng K, Joseph B, Gupta S. Spontaneous origin from human embryonic stem cells of liver cells displaying conjoint meso-endodermal phenotype with hepatic functions. Journal of Cell Science 2012;125(5):1274-1283. doi:10.1242/jcs.095372.
    Species: Human
    Sample type: Conditioned Media
  6. Inoue T, Sugiyama H, Kitagawa M, et al. Suppression of Adiponectin by Aberrantly Glycosylated IgA1 in Glomerular Mesangial Cells In Vitro and In Vivo. CÃ?mara NOS, ed. PLoS ONE. 2012;7(3):e33965. doi:10.1371/journal.pone.0033965.
    Species: Human
    Sample type: Conditioned Media
  7. Varchetta S, Brunetta E, Roberto A, et al. Engagement of Siglec-7 Receptor Induces a Pro-Inflammatory Response Selectively in Monocytes. Zissel G, ed. PLoS ONE. 2012;7(9):e45821. doi:10.1371/journal.pone.0045821.
    Species: Human
    Sample type: Conditioned Media
  8. Chen H., Wang Y., Bai C., Wang X. Alterations of plasma inflammatory biomarkers in the healthy and chronic obstructive pulmonary disease patients with or without acute exacerbation. J Proteomics. 2012 Jun 6;75(10):2835-43. doi: 10.1016/j.jprot.2012.01.027.
    Species: Human
    Sample type: Plasma
  9. Bian L., sun X., Jin K., He Y. Oral cancer-associated fibroblasts inhibit heat-induced apoptosis in Tca8113 cells through upregulated expression of Bcl-2 through the Mig/CXCR3 axis. Oncol Rep. 2012 Dec;28(6):2063-8. doi: 10.3892/or.2012.2019.
    Species: Human
    Sample type: Conditioned Media
  10. Edsbeg L., Wyffels J., Brogan M., Fries K. Analysis of the proteomic profile of chronic pressure ulcers. Wound Repair Regen. 2012 May-Jun;20(3):378-401. doi: 10.1111/j.1524-475X.2012.00791.x
    Species: Human
    Sample type: Wound Fluid
Perfect for secretome screening

Bern University Hospital, Inselspital
Easy to set up and analyze.

ATHERSYS, INC
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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