Rat L90 Array, Glass Slide

RayBio® L-Series Rat Antibody Array 90 Glass Slide Kit. Detects 90 Rat Proteins. Suitable for serum, plasma, and cell culture supernatants.

$950.41
AAR-BLG-1-4
+

Antigen Information

Gene Symbols:
ADIPOQ | AGER | BDNF | CCL2 | CCL20 | CCL22 | CCL3 | CCR4 | CD80 | CNTF   View more

Assay Format

Number of Targets Detected:
90
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Label-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Feature

  • High density arrays
  • 90 Rat proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use

Target Name

Activin A, ACTH, ADFP, Adiponectin, AMPK alpha 1, B7-1/CD80, BDNF, beta-Catenin, bFGF, b-NGF, CCR4, CD106, CINC-2 alpha/beta, CINC-3, CNTF, CNTF R alpha, CSK, CXCR4, EGFR, EG-VEGF/PK1, E-Selectin, FADD, Fas Ligand/TNFSF6, Fas/TNFRSF6, FGF-BP, Follostatin-like -1 (FSL1), Fractalkine, GFR alpha-1, GFR alpha-2, GM-CSF, Growth Hormone, Growth Hormone R, Hepassocin, ICAM-1/CD54, ICK, Insulin Degrading Enzyme (IDE), IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 R6/IL-1 R rp2, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12/IL-23 p40, IL-13, Integrin alpha M beta 2, Inuslin, IP-10, Leptin (OB), LIX, L-Selectin/CD62L, MCP-1, MDC, MIF, MIP-1 alpha, MIP-2, MIP-3 alpha, MMP-13, MMP-2, MMP-8, MuSK, Neuropilin-2, NGFR, Orexin A, Osteopontin/SPP1, PDGF-AA, Prolactin R, RAGE, RALT/MIG-6, RELM beta, Resistin, TL1A/TNFSF15/VEGI, TGF beta 1, TGF beta 2, TGF beta 3, Thrombospondin, TIE-2, TIMP-1, TIMP-2, TIMP-3, TLR4, TNF alpha, TRAIL, TROY, Ubiquitin+1, VEGF-A, VEGF-C

Target Species

Rat

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Rat 90 Antibody Array Glass Slide(s)
  • Dialysis Tubes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Streptavidin-Conjugated Fluor
  • Adhesive Plastic Strips
  • Serum Buffer
  • Floating Dialysis Rack
  • 30 ml Centrifuge Tube
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4 and Na2HPO4
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • 1 ml tube
  • Pipettors, pipette tips and other common lab consumables
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated samples using spin column
  4. Dry the glass slide
  5. Block array surface
  6. Incubate with Samples
  7. Incubate with Streptavidin-Conjugated Fluor
  8. Disassemble the glass slide
  9. Scan with a gene microarray laser scanner
  10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Wang, N., Li, Q., Zhang, L., Lin, H., Hu, J., Li, D., ... & Wan, J. (2012). Mesenchymal stem cells attenuate peritoneal injury through secretion of TSG-6. PloS one, 7(8), e43768.
    Species: Rat
    Sample type: Conditioned Media
  2. Li F., Chen B., Li W., et al. Islet Stellate Cells Isolated from Fibrotic Islet of Goto-Kakizaki Rats Affect Biological Behavior of Beta-Cell. J Diabetes Res. 2016; 2016: 6924593. Published online 2015 Dec 1. doi: 10.1155/2016/6924593
    Species: Rat
    Sample type: Conditioned Media
  3. Jian Q., Tao Z., Li Y., Yin ZQ. Acute retinal injury and the relationship between nerve growth factor, Notch1 transcription and short-lived dedifferentiation transient changes of mammalian Müller cells. Vision Res. 2015 May;110(Pt A):107-17. doi: 10.1016/j.visres.2015.01.030.
    Species: Rat
    Sample type: Tissue Lysate
  4. Sakai T, Xu Y. Stem Cells Decreased Neuronal Cell Death after Hypoxic Stress in Primary Fetal Rat Neurons in vitro. Cell Transplantation 2012;21(1):355-364. doi:10.3727/096368911X580545.
    Species: Rat
    Sample type: Conditioned Media
  5. Wang N, Li Q, Zhang L, et al. Mesenchymal Stem Cells Attenuate Peritoneal Injury through Secretion of TSG-6. Câmara NOS, ed. PLoS ONE. 2012;7(8):e43768. doi:10.1371/journal.pone.0043768.
    Species: Rat
    Sample type: Conditioned Media
  6. Yao L., Dong W., Lu F., Liu S. An Improved Acute Gouty Arthritis Rat Model and Therapeutic Effect of Rhizoma Dioscoreae Nipponicae on Acute Gouty Arthritis Based on the Protein-Chip Methods. Am J Chin Med. 2012;40(1):121-34.
    Species: Rat
    Sample type: Synovial Fluid
  7. Pickford, Fiona, et al. "Progranulin is a chemoattractant for microglia and stimulates their endocytic activity." The American journal of pathology 178.1 (2011): 284-295.
    Species: Rat
    Sample type: Conditioned Media

No posts found

Write A Review
U.S. Non-U.S.

Analysis Tool Bundle Discount

Specify Size
1 x $950.41
+
1 x $210.08 $63.02

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $1,160.49
Price for all $1,013.43

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program

Related Products Related Products