Rat L90 Array, Membrane

RayBio® L-Series Rat Antibody Array 90 Membrane Kit. Detects 90 Rat Proteins. Suitable for Serum, Plasma, Lysates, and Cell Culture Supernatants.

Cell Culture Media Manual Serum, Plasma, Lysate Manual

AAR-BLM-1A-2
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Antigen Information

Gene Symbols:
ADIPOQ | AGER | BDNF | CCL2 | CCL20 | CCL22 | CCL3 | CCR4 | CD80 | CNTF   View more

Assay Format

Number of Targets Detected:
90
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Label-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2 or 4 Sample Kit

Product Feature

  • High density arrays
  • 90 Rat proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use

Target Name

Activin A, ACTH, ADFP, Adiponectin, AMPK alpha 1, B7-1/CD80, BDNF, beta-Catenin, bFGF, b-NGF, CCR4, CD106, CINC-2 alpha/beta, CINC-3, CNTF, CNTF R alpha, CSK, CXCR4, EGFR, EG-VEGF/PK1, E-Selectin, FADD, Fas Ligand/TNFSF6, Fas/TNFRSF6, FGF-BP, Follostatin-like -1 (FSL1), Fractalkine, GFR alpha-1, GFR alpha-2, GM-CSF, Growth Hormone, Growth Hormone R, Hepassocin, ICAM-1/CD54, ICK, Insulin Degrading Enzyme (IDE), IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 R6/IL-1 R rp2, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12/IL-23 p40, IL-13, Integrin alpha M beta 2, Inuslin, IP-10, Leptin (OB), LIX, L-Selectin/CD62L, MCP-1, MDC, MIF, MIP-1 alpha, MIP-2, MIP-3 alpha, MMP-13, MMP-2, MMP-8, MuSK, Neuropilin-2, NGFR, Orexin A, Osteopontin/SPP1, PDGF-AA, Prolactin R, RAGE, RALT/MIG-6, RELM beta, Resistin, TL1A/TNFSF15/VEGI, TGF beta 1, TGF beta 2, TGF beta 3, Thrombospondin, TIE-2, TIMP-1, TIMP-2, TIMP-3, TLR4, TNF alpha, TRAIL, TROY, Ubiquitin+1, VEGF-A, VEGF-C

Target Species

Rat

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Rat 90 Antibody Array Membranes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Plastic Sheets
  • Floating Dialysis Rack
  • Wash Buffer 1
  • Wash Buffer 2
  • Spin Columns
  • Plastic Incubation Tray
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4 and Na2HPO4
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • Pipettors, pipette tips and other common lab consumables
  • 2~5 ml tube
  • 50 ml conical collection tube
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated Samples in PBS
  4. Block array membranes
  5. Incubate with Samples
  6. Incubate with HRP-Conjugated Streptavidin
  7. Incubate with Detection Buffers
  8. Image with chemiluminescent imaging system
  9. Perform densitometry and analysis

Storage/Stability

For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. He H, Zhao Z-H, Han F-S, Wang X-F, Zeng Y-J. Activation of protein kinase C ? enhanced movement ability and paracrine function of rat bone marrow mesenchymal stem cells partly at least independent of SDF-1/CXCR4 axis and PI3K/AKT pathway. International Journal of Clinical and Experimental Medicine. 2015;8(1):188-202.
    Species: Rat
    Sample type: Conditioned Media
  2. Chen H., Min XH., Wang QY., et al. Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury. Scientific Reports 5, Article number: 8718 (2015). doi:10.1038/srep08718
    Species: Rat
    Sample type: Conditioned Media
  3. Browne S., Monaghan M., Brauchle E., et al. Modulation of inflammation and angiogenesis and changes in ECM GAG-activity via dual delivery of nucleic acids. Biomaterials. 2015 Nov;69:133-47. doi: 10.1016/j.biomaterials.2015.08.012.
    Species: Rat
    Sample type: Tissue Lysate
  4. Wang Y., et al. Cinnabar-induced Subchronic Renal Injury is Associated with Increased Apoptosis in Rats. Biomed Research International Sept 2014 Epub Ahead of print.
    Species: Rat
    Sample type: Tissue Lysate
  5. Noiseux N., Borie M., Desnoyers A., et al. Preconditioning of stem cells by oxytocin to improve their therapeutic potential. Endocrinology. 2012 Nov;153(11):5361-72. doi: 10.1210/en.2012-1402'
    Species: Rat
    Sample type: Conditioned Media
  6. Zhou LY., Wang ZM., Gao YB., et al. Stimulation of hepatoma cell invasiveness and metastatic potential by proteins secreted from irradiated nonparenchymal cells. Int J Radiat Oncol Biol Phys. 2012 Nov 1;84(3):822-8. doi: 10.1016/j.ijrobp.2012.01.011
    Species: Rat
    Sample type: Conditioned Media
  7. Vause, C. Durham, P. Identification of Cytokines and Signaling Proteins Differentially Regulated by Sumatriptan/Naproxen. Headache. 2012 Jan;52(1):80-9. doi: 10.1111/j.1526-4610.2011.02048.x. Epub 2011 Dec 8.
    Species: Rat
    Sample type: Tissue Lysate
  8. Sun Z., Wang Y., Gong X., et al. Secretion of rat tracheal epithelial cells induces mesenchymal stem cells to differentiate into epithelial cell. Cell Biol Int. 2012 Feb;36(2):169-75. doi: 10.1042/CBI20110121.
    Species: Rat
    Sample type: Conditioned Media
  9. Vause, C. V. and Durham, P. L. (2012), Identification of Cytokines and Signaling Proteins Differentially Regulated by Sumatriptan/Naproxen. Headache: The Journal of Head and Face Pain, 52: 80-89. doi: 10.1111/j.1526-4610.2011.02048.x
    Species: Rat
    Sample type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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