L-Series: Label-Based Antibody Arrays

Semi-quantitative, Label-based, High Density Antibody Arrays

Introducing the Human L2000 Array Simultaneously detect 2000 proteins using our largest array yet

Combining direct antigen-labeling technology with our vast library of array-validated antibodies, RayBiotech has created our largest antibody array to date: the Human L2000 Array. With the L-Series high density array platform, researchers can now detect up to 2000 proteins simultaneously, obtaining a broad, panoramic view of protein expression. Our newly expanded panel includes a wide variety of metabolic enzymes, structural proteins, epigenetic markers, neuroregulatory factors, in addition to our popular list of cytokines, growth factors, receptors, adipokines, proteases, and signaling proteins. Available on both glass slide and membrane formats, this array is ideally suited for biomarker discovery studies and exploratory screens.

For a comparison & overview of all our antibody array systems, click here.

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Features

  • High detection sensitivity
  • Easy to use; obtain results in 2 days
  • No need for fractionation, depletion or extraction of samples
  • Consumes only 20-30 ul of serum/plasma
  • No dedicated equipment needed (glass slide or membrane)
  • Accurate and reproducible
  • As little as 59¢ per target

Research Applications

  • High throughput expression profiling
  • Discovering potential molecular targets for drug development
  • Uncovering molecular mechanisms of drug action
  • Identifying crucial factors involved in disease processes
  • Discovering biomarkers for disease management
  • Discovering expression patterns for molecular classification of diseases

How It Works

Through a simple labeling process, the sample proteins are directly conjugated to biotin, eliminating the need for a second antibody to develop the array signals. In this format, unintended antibody interactions are impossible, thus eliminating limitations on the size of the array panel.

Illustration of Label-Based Detection

Our RayBio® Label-Based Arrays differ from our sandwich ELISA-based arrays primarily in the means of detection. Instead of using a second biotinylated antigen-specific antibody, sample proteins themselves are labeled with biotin prior to incubation with the capture antibodies. Proteins binding to the array can be detected using streptavidin-HRP for chemiluminescence detection or streptavidin-conjugated HiLyte Fluor 532™ for fluorescence detection.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

Contents of Kit

  • RayBio® L-Series Antibody Array Membranes or Glass Slides
  • Spin Columns / Dialysis Tube
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Streptavidin-Conjugated HRP or Streptavidin-Conjugated HiLyte Fluor 532
  • Wash Buffer 1
  • Wash Buffer 2
  • Plastic Sheets
  • Floating Dialysis Rack
  • Plastic Incubation Tray
  • Detection Buffer C
  • Detection Buffer D

*Accessories include: 2-well or 4-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

Assay Diagram

The cell culture supernates or serum is dialyzed before biotin-labeling step. Through a simple process, the primary amine of the proteins in the sample is biotinylated, followed by dialysis to remove free biotin. From here, the newly biotinylated sample is added onto the array membrane or glass slide and incubated at room temperature. After incubation with HRP-streptavidin or Fluorescent Dye-Strepavidin, the signals can be visualized either by chemiluminescence or fluorescence. The experimental procedure is simple and can be performed in any laboratory.

Representative Data

A) Human L-507 Array (Glass Slide Format | Membrane Format)

The figure shows the RayBio® Biotin-label-based Human Antibody Array 507 probed with two cell culture supernates. The image were captured using a Axon GenePix laser scanner.

B) Mouse L-308 Array (Glass Slide Format | Membrane Format)

The figure shows the RayBio® Biotin-label-based Mouse Array 308 probed with Mouse sample. The image were captured using a Axon GenePix laser scanner.

References

  • Lee N., Park S., Kwon S., et al. Agouti Related Peptide Secreted Via Human Mesenchymal Stem Cells Upregulates Proteasome Activity in an Alzheimer’s Disease Model. Sci Rep. 2017; 7: 39340. Jan 4. doi: 10.1038/srep39340 [view publication]
    Product: Human L507 Array, Glass Slide
    Sample Type: Conditioned Media
  • Liu Y., Conboy M., Mehdipour M., et al. Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis. Nat Commun. 2017; 8: 643. doi: 10.1038/s41467-017-00698-y [view publication]
    Product: Mouse L308 Array, Membrane
    Sample Type: Tissue Lysate
  • Wang S., Lu Y., Hong Q., et al. Protein Array-Based Detection of Proteins in Kidney Tissues from Patients with Membranous Nephropathy. Biomed Res Int. 2017; 2017: 7843584. doi: 10.1155/2017/7843584 [view publication]
    Products: Human L507 Array, Glass Slide | Human L1000 Array, Glass Slide | Human L493 Array, Glass Slide
    Sample Type: Tissue Lysate
  • Wang, N., Li, Q., Zhang, L., Lin, H., Hu, J., Li, D., ... & Wan, J. (2012). Mesenchymal stem cells attenuate peritoneal injury through secretion of TSG-6. PloS one, 7(8), e43768. [view publication]
    Product: Rat L90 Array, Glass Slide
    Sample Type: Conditioned Media
  • Duckworth C., Zhang L., Carroll S., et al. Overexpression of GAB2 in ovarian cancer cells promotes tumor growth and angiogenesis by upregulating chemokine expression. Oncogene. 2016 Aug 4;35(31):4036-47. doi: 10.1038/onc.2015.472. [view publication]
    Product: Human L1000 Array, Membrane
    Sample Type: Conditioned Media
  • Ge H., Zhu H., Xu N., et al. Increased Lung Ischemia-Reperfusion Injury in Aquaporin 1-Null Mice Is Mediated via Decreased Hypoxia-Inducible Factor 2α Stability. Am J Respir Cell Mol Biol. 2016 Jun;54(6):882-91. doi: 10.1165/rcmb.2014-0363OC. [view publication]
    Product: Mouse L308 Array, Glass Slide
    Sample Type: Tissue lysate
  • Li F., Chen B., Li W., et al. Islet Stellate Cells Isolated from Fibrotic Islet of Goto-Kakizaki Rats Affect Biological Behavior of Beta-Cell. J Diabetes Res. 2016; 2016: 6924593. Published online 2015 Dec 1. doi: 10.1155/2016/6924593 [view publication]
    Product: Rat L90 Array, Glass Slide
    Sample Type: Conditioned Media
  • Lu P., Zheng D., Fang C., et al. Cytokines in cerebrospinal fluid of neurosyphilis patients: Identification of Urokinase plasminogen activator using antibody microarrays. J Neuroimmunol. 2016 Apr 15;293:39-44. doi: 10.1016/j.jneuroim.2015.12.010. [view publication]
    Product: Human L507 Array, Glass Slide
    Sample Type: CSF
  • Meltzer S., Kalanxhi E., Hektoen H., et al. Systemic release of osteoprotegerin during oxaliplatincontaining induction chemotherapy and favorable systemic outcome of sequential radiotherapy in rectal cancer. Oncotarget. 2016 Apr 26. doi: 10.18632/oncotarget.8995 [view publication]
    Product: Human L507 Array, Glass Slide
    Sample Type: Serum
  • Pal S., Nguyen C., Morita K., et al. THBS1 is induced by TGFB1 in the cancer stroma and promotes invasion of oral squamous cell carcinoma. J Oral Pathol Med. 2016 Feb 5. doi: 10.1111/jop.12430. [view publication]
    Product: Human L507 Array, Membrane
    Sample Type: Conditioned Media
  • Qu X., Zhou J., Wang T., et al. MiR-30a inhibits Th17 differentiation and demyelination of EAE mice by targeting the IL-21R. Brain Behav Immun. 2016 Oct;57:193-9. doi: 10.1016/j.bbi.2016.03.016. [view publication]
    Product: Human L507 Array, Glass Slide
    Sample Type: Serum
  • Torre-Minguela C., Barbera-cremades M., Gomex A., et al. Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process. Scientific Reports 6, Article number: 22586 (2016) doi:10.1038/srep22586 [view publication]
    Product: Mouse L308 Array, Membrane
    Sample Type: Conditioned Media
  • Williams K., Killebrew D., Clary G., Meeker R. Opposing Effects of NGF and proNGF on HIV Induced Macrophage Activation. J Neuroimmune Pharmacol. 2016 Mar;11(1):98-120. doi: 10.1007/s11481-015-9631-z [view publication]
    Product: Human L507 Array, Glass Slide
    Sample Type: Conditioned Media
  • Browne S., Monaghan M., Brauchle E., et al. Modulation of inflammation and angiogenesis and changes in ECM GAG-activity via dual delivery of nucleic acids. Biomaterials. 2015 Nov;69:133-47. doi: 10.1016/j.biomaterials.2015.08.012. [view publication]
    Product: Rat L90 Array, Membrane
    Sample Type: Tissue Lysate
  • Carolina Torres, Ana Linares, Maria José Alejandre, et al., “Prognosis Relevance of Serum Cytokines in Pancreatic Cancer,” BioMed Research International, Article ID 518284, in press. [view publication]
    Product: Human L507 Array, Membrane
    Sample Type: Serum

More references are listed per product on the individual product pages.