L-Series: Label-Based Antibody Arrays

Semi-quantitative, Label-based, High Density Antibody Arrays

Introducing the Human L2000 Array Simultaneously detect 2000 proteins using our largest array yet

Combining direct antigen-labeling technology with our vast library of array-validated antibodies, RayBiotech has created our largest antibody array to date: the Human L2000 Array. With the L-Series high density array platform, researchers can now detect up to 2000 proteins simultaneously, obtaining a broad, panoramic view of protein expression. Our newly expanded panel includes a wide variety of metabolic enzymes, structural proteins, epigenetic markers, neuroregulatory factors, in addition to our popular list of cytokines, growth factors, receptors, adipokines, proteases, and signaling proteins. Available on both glass slide and membrane formats, this array is ideally suited for biomarker discovery studies and exploratory screens.

For a comparison & overview of all our antibody array systems, click here.

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Discovery Proteomics Services

Let Us Run L-Series For You

Ship us your samples (4 sample minimum) and our experienced service department will perform a high-density array of your choice. We can analyze up to 2000 human, 1308 mouse, or 90 rat proteins and return to you a full data report. Click the button below to learn more.

Discovery Proteomics Services

Features

  • High detection sensitivity
  • Easy to use; obtain results in 2 days
  • No need for fractionation, depletion or extraction of samples
  • Consumes only 20-30 ul of serum/plasma
  • No dedicated equipment needed (glass slide or membrane)
  • Accurate and reproducible
  • As little as 59¢ per target

Research Applications

  • High throughput expression profiling
  • Discovering potential molecular targets for drug development
  • Uncovering molecular mechanisms of drug action
  • Identifying crucial factors involved in disease processes
  • Discovering biomarkers for disease management
  • Discovering expression patterns for molecular classification of diseases

How It Works

Through a simple labeling process, the sample proteins are directly conjugated to biotin, eliminating the need for a second antibody to develop the array signals. In this format, unintended antibody interactions are impossible, thus eliminating limitations on the size of the array panel.

Illustration of Label-Based Detection

Our RayBio® Label-Based Arrays differ from our sandwich ELISA-based arrays primarily in the means of detection. Instead of using a second biotinylated antigen-specific antibody, sample proteins themselves are labeled with biotin prior to incubation with the capture antibodies. Proteins binding to the array can be detected using streptavidin-HRP for chemiluminescence detection or streptavidin-conjugated fluor for fluorescence detection.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

Contents of Kit

  • RayBio® L-Series Antibody Array Membranes or Glass Slides
  • Spin Columns / Dialysis Tube
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Streptavidin-Conjugated HRP or Streptavidin-Conjugated Fluor
  • Wash Buffer 1
  • Wash Buffer 2
  • Plastic Sheets
  • Floating Dialysis Rack
  • Plastic Incubation Tray
  • Detection Buffer C
  • Detection Buffer D

*Accessories include: 2-well or 4-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

Assay Diagram

The cell culture supernates or serum is dialyzed before biotin-labeling step. Through a simple process, the primary amine of the proteins in the sample is biotinylated, followed by dialysis to remove free biotin. From here, the newly biotinylated sample is added onto the array membrane or glass slide and incubated at room temperature. After incubation with HRP-streptavidin or Fluorescent Dye-Strepavidin, the signals can be visualized either by chemiluminescence or fluorescence. The experimental procedure is simple and can be performed in any laboratory.

Representative Data

A) Human L-507 Array (Glass Slide Format | Membrane Format)

The figure shows the RayBio® Biotin-label-based Human Antibody Array 507 probed with two cell culture supernates. The image were captured using a Axon GenePix laser scanner.

B) Mouse L-308 Array (Glass Slide Format | Membrane Format)

The figure shows the RayBio® Biotin-label-based Mouse Array 308 probed with Mouse sample. The image were captured using a Axon GenePix laser scanner.

References

  • Wolford C., McConoughey S., Jalgaonkar S., et al. Transcription factor ATF3 links host adaptive response to breast cancer metastasis. J Clin Invest. 2013 Jul 1; 123(7): 2893-2906. doi: 10.1172/JCI64410 [view publication]
    Product: Mouse L308 Array, Membrane
  • Liu Y., Conboy M., Mehdipour M., et al. Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis. Nat Commun. 2017; 8: 643. doi: 10.1038/s41467-017-00698-y [view publication]
    Product: Mouse L308 Array, Membrane
  • Tang YL, Zhu WW, Cheng M, Chen LJ, Zhang J, et al. Hypoxic Preconditioning Enhances the Benefit of Cardiac Progenitor Cell Therapy for Treatment of Myocardial Infarction by Inducing CXCR4 Expression. Circ Res. 2009;104:1209-1216. DOI: 10.1161/CIRCRESAHA.109.197723 [view publication]
    Product: Mouse L308 Array, Glass Slide
  • Scheel, Christina, et al. "Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast." Cell 145.6 (2011): 926-940. [view publication]
    Product: Human L507 Array, Membrane
  • Ryu JC, Davidson BP, xie A, et al. Molecular imaging of the paracrine proangiogenic effects of progenitor cell therapy in limb ischemia. Circulation. 2013 Feb 12;127(6):710-719. [view publication]
    Product: Human L507 Array, Membrane
  • Soria-Valles C., Osorio F., Gutierrez-Fernandez A., et al. NF-κB activation impairs somatic cell reprogramming in ageing. Nat Cell Biol. 2015 Aug;17(8):1004-13. doi: 10.1038/ncb3207 [view publication]
    Product: Human L1000 Array, Glass Slide | Human L507 Array, Glass Slide | Human L493 Array, Glass Slide
  • Straussman R., Morikawa T., et al. Tumourmicro-environment elicits innate resistance to RAF inhibitors through HGF secretion. Nature. 2012 Jul 26;487(7408):500-4. doi: 10.1038/nature11183. [view publication]
    Product: Human L507 Array, Glass Slide
  • Lim, S., Yoon, H. Y., Park, S.-J., Song, S., Shim, M. K., Yang, S., … Kim, K. (2021). Predicting in vivo therapeutic efficacy of bioorthogonally labeled endothelial progenitor cells in hind limb ischemia models via non-invasive fluorescence molecular tomography. Biomaterials, 266, 120472. doi:10.1016/j.biomaterials.2020.120472  [view publication]
    Product: Human L507 Array, Membrane
  • Lu H, Yang HL, Zhou WJ, Lai ZZ, Qiu XM, Fu Q, Zhao JY, Wang J, Li DJ, Li MQ. Rapamycin prevents spontaneous abortion by triggering decidual stromal cell autophagy-mediated NK cell residence. Autophagy. 2020 Nov 1:1-17. doi: 10.1080/15548627.2020.1833515. Epub ahead of print. PMID: 33030400. [view publication]
    Product: Human L1000 Array, Glass Slide

More references are listed per product on the individual product pages.