ELISA vs. Immuno-PCR vs. SIMOA: Comparison of Protein Detection Tools

ELISA vs. Immuno-PCR vs. SIMOA: Comparison of Protein Detection Tools

Introduction ELISA, or enzyme-linked immunosorbent assay, is the “gold standard” method for protein detection.1 In this blog, three popular ELISA platforms are reviewed: standard ELISA, immuno-PCR, and single molecule array (SIMOA). These ELISAs will be compared regarding their design principles, sample volumes, instruments, and sensitivities. How ELISA works The basic…

Multiplex Protein Detection with Antibody Arrays

Multiplex Protein Detection with Antibody Arrays

Introduction A microarray is an assay that enables the simultaneous detection of multiple molecules, such as nucleic acids, peptides, proteins, or antibodies. They are called “micro”-arrays because the analytes are measured within a small surface area; for example, thousands of molecules can be analyzed on a standard glass slide (75…

5 Steps for Writing a Research Paper

5 Steps for Writing a Research Paper

Introduction Publications are the cornerstone of science. They disseminate data that expand our understanding of the world around us (and beyond!) and help secure funding for additional studies. However, most manuscripts do not pass the journal’s initial editorial process. Here, we review the editorial process and provide 5 steps for…

The Case For NTD and S2: Prevalence and Protectiveness of Antibodies Towards SARS-CoV-2 Non-RBD Spike Epitopes

The Case For NTD and S2: Prevalence and Protectiveness of Antibodies Towards SARS-CoV-2 Non-RBD Spike Epitopes

Introduction Since the emergence of SARS-CoV-2, the causative agent of COVID-19, many researchers continue to focus their efforts on the discovery of potent neutralizing antibodies that would prove useful in the design of COVID-19 countermeasures such as vaccines and therapeutics. To date, most vaccines or therapeutics in clinical trials or…