Discovery in the Post-Genomic Age
How to use proteomic assays to discover more and publish faster
Introduction A microarray is an assay that enables the simultaneous detection of multiple molecules, such as nucleic acids, peptides, proteins, or antibodies. They are called “micro”-arrays because the analytes are measured within a small surface area; for example, thousands of molecules can be analyzed on a standard glass slide (75…
Highlights Antibody arrays: removal of high abundance proteins is not required, but antibody availability limits protein detection. Mass spectrometry: “hypothesis-free” protein detection is affected by high abundance proteins and peptide ionization. Both high throughput technologies are capable of semi-quantitative and quantitative data. Antibody arrays and mass spectrometry complement each other.…
Exosomes are 30-150 nm extracellular vesicles that are endocytically released from cells. Exosomes were previously thought to simply exude unnecessary proteins from cells; however, more recent research has highlighted their importance in cell-to-cell communication by transporting biomolecules such as mRNA, microRNA, and protein through the circulatory system. Recipient cells may…
In the January 2014 Issue of Clinical Laboratory News, published by the AACC (American Association for Clinical Chemistry), one of the cover stories was The Search for Improved Markers of Acute Kidney Injury. The article’s main points are that: The current markers used to assess acute kidney injury (AKI), creatinine…