ELISA vs. Immuno-PCR vs. SIMOA: Comparison of Protein Detection Tools

ELISA vs. Immuno-PCR vs. SIMOA: Comparison of Protein Detection Tools

Introduction ELISA, or enzyme-linked immunosorbent assay, is the “gold standard” method for protein detection.1 In this blog, three popular ELISA platforms are reviewed: standard ELISA, immuno-PCR, and single molecule array (SIMOA). These ELISAs will be compared regarding their design principles, sample volumes, instruments, and sensitivities. How ELISA works The basic…

Preparing Conditioned Media Samples for ELISA Kits

ELISA Kit RayBiotech manufactures over 1,000 high quality, rigorously tested ELISA kits. In this blog post we explain how to prepare conditioned media samples for your ELISA Kit. How to Prepare Conditioned Media Samples We recommend preparing serum-free or low-serum medium samples, as serum tends to contain cytokines which may…

Preparing Plasma and Serum Samples for ELISA Kits

RayBiotech manufactures over 1,000 high fully validated, GMP-compliant ELISA kits. In this blog post we explain how to prepare plasma and serum samples for ELISA Kits. How to Prepare plasma and serum samples for ELISA Kits For plasma: Collect whole blood into an EDTA, Citrate or Sodium heparin tube (e.g.…

Preparing cell or tissue lysates for ELISA Kits

RayBiotech manufactures over 2,000 high fully validated, GMP-compliant ELISA kits. In this blog post we explain how to prepare cell or tissue lysates for ELISA Kits. Preparing cell or tissue lysates for ELISA Kits Cell or tissue lysates for use with RayBio® ELISA kits can be prepared using most conventional…