Preparing Plasma and Serum Samples for ELISA Kits


ELISA Kits

RayBiotech manufactures over 1,000 high fully validated, GMP-compliant ELISA kits. In this blog post we explain how to prepare plasma and serum samples for ELISA Kits.

How to Prepare plasma and serum samples for ELISA Kits

For plasma:
  1. Collect whole blood into an EDTA, Citrate or Sodium heparin tube (e.g. BD vacutainer, Cat # 8001302 or 16852).
  2. Centrifuge 10 minutes at 3,000 rpm
  3. Aliquot into small tubes and store at -80°C until use.
For serum:
  1. Collect whole blood into a tube without additives (e.g. BD vacutainer, Cat # 8002527).
  2. Keep at room temperature for 20 minutes.
  3. Centrifuge 10 minutes at 3,000 rpm.
  4. Aliquot into small tubes and store at -80°C until use.

Note: Regardless of the sample type you have, it is strongly recommended that you sub-aliquot all samples after preparation to minimize protein degradation from multiple freeze-thaw cycles. This also ensures availability of sample for further experiments.

For more information about our ELISA Kits and sample preparation, visit www.raybiotech.com.

3 Feedbacks on “Preparing Plasma and Serum Samples for ELISA Kits”

    1. For a 96-well ELISA, generally 100ul of total volume is required for each well. However, the amount of plasma within that total volume can vary based on the specific target being measured, as the abundance of proteins can vary in biological samples. To get the target protein concentration within the detectable range of the assay, more or less dilution of the plasma sample can be required. Typically at least a 2x dilution (so needing 50ul of straight plasma) is required for each well. But please look at the manufacturer’s recommendation for suggested dilutions or test several dilutions in your specific sample to optimize the needed amount.

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